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Open AccessArticle

Post-Modified Polypeptides with UCST-Type Behavior for Control of Cell Attachment in Physiological Conditions

School of Pharmacy, the University of Nottingham, University Park, Nottingham NG7 2RD, UK
School of Materials, the University of Manchester, Manchester M13 9PL, UK
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Materials 2018, 11(1), 95;
Received: 29 November 2017 / Revised: 31 December 2017 / Accepted: 5 January 2018 / Published: 9 January 2018
(This article belongs to the Special Issue Temperature-Responsive Polymers)
Upper Critical Solution Temperature (UCST)-type thermally responsive polypeptides (TRPs) with phase transition temperatures around 37 °C in phosphate-buffered saline (PBS) buffer (pH 7.4, 100 mM) were prepared from poly(l-ornithine) hydrobromide and coated on non-tissue culture-treated plastic plates (nTCP). Cell adhesion was observed at temperatures above the phase transition temperature of the coating polymer (39 °C), while cell release was triggered when the culture temperature was switched to 37 °C. Approximately 65% of the attached cells were released from the surface within 6 h after changing the temperature, and more than 96% of the released cells were viable. Water contact angle measurements performed at 39 and 37 °C demonstrated that the surface hydrophobicity of the new TRP coatings changed in response to applied temperature. The cell attachment varied with the presence of serum in the media, suggesting that the TRP coatings mediated cell attachment and release as the underlying polymer surface changed conformation and consequently the display of adsorbed protein. These new TRP coatings provide an additional means to mediate cell attachment for application in cell-based tissue regeneration and therapies. View Full-Text
Keywords: UCST polymer; thermo-responsive polypeptides; controlled cell attachment UCST polymer; thermo-responsive polypeptides; controlled cell attachment
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MDPI and ACS Style

Xue, X.; Thiagarajan, L.; Dixon, J.E.; Saunders, B.R.; Shakesheff, K.M.; Alexander, C. Post-Modified Polypeptides with UCST-Type Behavior for Control of Cell Attachment in Physiological Conditions. Materials 2018, 11, 95.

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