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Materials 2018, 11(1), 21; https://doi.org/10.3390/ma11010021

Effect of the Heat-Treated Ti6Al4V Alloy on the Fibroblastic Cell Response

1
Instituto Politécnico Nacional, Departamento de Ingeniería en Metalurgia y Materiales, UPALM Edificio 7, Mexico City 07738, Mexico
2
Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Departamento de Ingeniería de Superficies, Corrosión y Durabilidad, Madrid 28040, Spain
3
Instituto Politécnico Nacional, Departamento de Ingeniería Química Industrial, UPALM Edificio 7, Mexico City 07738, Mexico
*
Author to whom correspondence should be addressed.
Received: 23 November 2017 / Revised: 13 December 2017 / Accepted: 22 December 2017 / Published: 30 December 2017
(This article belongs to the Section Biomaterials)
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Abstract

Two heat treatments were carried out below (Ti6Al4V800) and above (Ti6Al4V1050) Ti6Al4V beta-phase transformation temperature (980 °C), with the purpose of studying the effect of microstructure on the adhesion and proliferation of fibroblast cells, as well as their electrochemical behavior. These alloys were seeded with 10,000 L929 fibroblast cells and immersed for 7 days in the cell culture at 37 °C, pH 7.40, 5% CO2 and 100% relative humidity. Cell adhesion was characterized by Scanning Electron Microscopy (SEM) and Electrochemical Impedance Spectroscopy (EIS) techniques. Polygonal and elongated cell morphology was observed independent of Ti6Al4V microstructure. Besides, C, O, P, S, Na and Cl signals were detected by Energy Dispersive X-Ray Spectroscopy (EDX), associated with the synthesis of organic compounds excreted by the cells, including protein adsorption from the medium. In certain areas on Ti6Al4V and Ti6Al4V800 alloys, cells were agglomerated (island type), likely related to the globular microstructure; meanwhile, larger cellular coverage is shown for Ti6Al4V1050 alloy, forming more than one layer on the surface, where only Ca was recorded. Impedance diagrams showed a similar passive behavior for the different Ti6Al4V alloys, mainly due to TiO2 overlaying the contribution of the organic compounds excreted by fibroblast cells. View Full-Text
Keywords: Ti6Al4V; heat treatment; fibroblasts Ti6Al4V; heat treatment; fibroblasts
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Chávez-Díaz, M.P.; Escudero-Rincón, M.L.; Arce-Estrada, E.M.; Cabrera-Sierra, R. Effect of the Heat-Treated Ti6Al4V Alloy on the Fibroblastic Cell Response. Materials 2018, 11, 21.

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