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Correction published on 1 July 2014, see Energies 2014, 7(7), 4199-4201.

Open AccessArticle
Energies 2014, 7(6), 3872-3885;

Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi

Swedish Centre for Resource Recovery, University of Borås, Borås SE-50190, Sweden
Author to whom correspondence should be addressed.
Received: 22 April 2014 / Revised: 17 May 2014 / Accepted: 17 June 2014 / Published: 20 June 2014
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A starch-based ethanol facility producing 200,000 m3 ethanol/year also produces ca. 2 million m3 thin stillage, which can be used to improve the entire process. In this work, five food-grade filamentous fungi, including a Zygomycete and four Ascomycetes were successfully grown in thin stillage containing 9% solids. Cultivation with Neurospora intermedia led to the production of ca. 16 g·L−1 biomass containing 56% (w/w) crude protein, a reduction of 34% of the total solids, and 5 g·L−1 additional ethanol. In an industrial ethanol production process (200,000 m3 ethanol/year), this can potentially lead to the production of 11,000 m3 extra ethanol per year. Cultivation with Aspergillus oryzae resulted in 19 g·L−1 biomass containing 48% (w/w) crude protein and the highest reduction of the thin stillage glycerol (54%) among the Ascomycetes. Cultivation with Rhizopus sp. produced up to 15 g·L−1 biomass containing 55% (w/w) crude protein. The spent thin stillage had been reduced up to 85%, 68% and 21% regarding lactic acid, glycerol and total solids, respectively. Therefore, N. intermedia, in particular, has a high potential to improve the ethanol process via production of additional ethanol and high-quality biomass, which can be considered for animal feed applications such as for fish feed. View Full-Text
Keywords: Ascomycetes; biomass; ethanol; protein; thin stillage; Zygomycetes Ascomycetes; biomass; ethanol; protein; thin stillage; Zygomycetes

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Ferreira, J.A.; Lennartsson, P.R.; Taherzadeh, M.J. Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi. Energies 2014, 7, 3872-3885.

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