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Article

The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure

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Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland
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Department of Physiology, Genetics and Plant Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-957 Olsztyn, Poland
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Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland
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Environmental Biotechnology Department, Faculty of Energy and Environmental Engineering, Silesian University of Technology, Akademicka 2 Str., 44-100 Gliwice, Poland
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The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-100 Gliwice, Poland
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Department of Environmental Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Warszawska 117 Str., 10-950 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Elia Judith Martínez Torres
Energies 2021, 14(4), 1125; https://doi.org/10.3390/en14041125
Received: 26 January 2021 / Revised: 12 February 2021 / Accepted: 12 February 2021 / Published: 20 February 2021
This study explored the effect of eight antimicrobials on the efficiency of biogas production in the anaerobic digestion (AD) process of cattle manure. The microbiome involved in AD, presence and number of genes mcrA, MSC and MST specific for Archaea, and antibiotic resistance genes (ARGs) concentration in digestate (D) were examined. Supplementation of antibiotics to substrate significantly lowered biogas production. Amoxicillin caused a 75% decrease in CH4 production in comparison with the control samples. Enrofloxacin, tetracycline, oxytetracycline, and chlortetracycline reduced the amount of biogas produced by 36, 39, 45 and 53%, respectively. High-throughput sequencing of 16S rRNA results revealed that bacteria dominated the Archaea microorganisms in all samples. Moreover, antibiotics led to a decrease in the abundance of the genes mcrA, MSC, MST, and induced an increase in the number of tetracyclines resistance genes. Antibiotics decreased the efficiency of the AD process and lowered the quantity of CH4 obtained, while stimulating an increase in the number of ARGs in D. This work reveals how antimicrobials affect the cattle manure AD process and changes in microbial biodiversity, number of functional genes and ARGs in the digestate due to drugs exposure. It also, provides useful, practical information about the AD process. View Full-Text
Keywords: mesophilic anaerobic digestion; biogas production; microbiome biodiversity; antibiotic resistance genes; Illumina MiSeq Sequencing mesophilic anaerobic digestion; biogas production; microbiome biodiversity; antibiotic resistance genes; Illumina MiSeq Sequencing
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MDPI and ACS Style

Koniuszewska, I.; Harnisz, M.; Korzeniewska, E.; Czatzkowska, M.; Jastrzębski, J.P.; Paukszto, Ł.; Bajkacz, S.; Felis, E.; Rusanowska, P. The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure. Energies 2021, 14, 1125. https://doi.org/10.3390/en14041125

AMA Style

Koniuszewska I, Harnisz M, Korzeniewska E, Czatzkowska M, Jastrzębski JP, Paukszto Ł, Bajkacz S, Felis E, Rusanowska P. The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure. Energies. 2021; 14(4):1125. https://doi.org/10.3390/en14041125

Chicago/Turabian Style

Koniuszewska, Izabela, Monika Harnisz, Ewa Korzeniewska, Małgorzata Czatzkowska, Jan P. Jastrzębski, Łukasz Paukszto, Sylwia Bajkacz, Ewa Felis, and Paulina Rusanowska. 2021. "The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure" Energies 14, no. 4: 1125. https://doi.org/10.3390/en14041125

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