Golgi Phosphoprotein 3 Mediates Radiation-Induced Bystander Effect via ERK/EGR1/TNF-α Signal Axis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Irradiation
2.3. Model of RIBE In Vitro
2.4. Establishment of Stable Cell Lines
2.5. Rescue Experiment
2.6. Knockout of GOLPH3 by Using CRISPR/Cas9
2.7. Western Blotting
2.8. ELISA Assay
2.9. Micronucleus (MN) Test
2.10. Quantitative Real-Time PCR (qPCR)
2.11. Immunofluorescence
2.12. Statistical Analysis
3. Results
3.1. GOLPH3 Plays a Key Role in the Production of RIBE Signals Released from Irradiated Cells
3.2. TNF-α Acts as an Intercellular Molecule in GOLPH3-Mediated RIBE
3.3. GOLPH3 Regulates the Transcription of TNF-α via ERK/EGR1 Pathway
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Qin, F.; Chen, G.; Yu, K.N.; Yang, M.; Cao, W.; Kong, P.; Peng, S.; Sun, M.; Nie, L.; Han, W. Golgi Phosphoprotein 3 Mediates Radiation-Induced Bystander Effect via ERK/EGR1/TNF-α Signal Axis. Antioxidants 2022, 11, 2172. https://doi.org/10.3390/antiox11112172
Qin F, Chen G, Yu KN, Yang M, Cao W, Kong P, Peng S, Sun M, Nie L, Han W. Golgi Phosphoprotein 3 Mediates Radiation-Induced Bystander Effect via ERK/EGR1/TNF-α Signal Axis. Antioxidants. 2022; 11(11):2172. https://doi.org/10.3390/antiox11112172
Chicago/Turabian StyleQin, Feng, Guodong Chen, Kwan Ngok Yu, Miaomiao Yang, Wei Cao, Peizhong Kong, Shengjie Peng, Mingyu Sun, Lili Nie, and Wei Han. 2022. "Golgi Phosphoprotein 3 Mediates Radiation-Induced Bystander Effect via ERK/EGR1/TNF-α Signal Axis" Antioxidants 11, no. 11: 2172. https://doi.org/10.3390/antiox11112172