Correction: Chudobova et al. Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus. Int. J. Environ. Res. Public Health 2014, 11, 3233–3255

There was an error in the original publication [1]. We were notified that in Figure 5A, we inadvertently used the wrong working version of agarose gel that remained in the article throughout the pre-peer review and peer review process. We were able to immediately locate the correct datasets. The corrected figure, as well as the specific part of the Results and Discussion, can be found below:

The fluorescence intensity of the amplified fragment varies in dependence on the antibiotic type. In the RPb strains, the zntR gene’s expression was higher than that of control S. aureus. In contrast, in the RCd, the expression of the zntR gene was similar to the control S. aureus strain. In the RCd strain, after the addition of AMP and TTC, the expression of the zntR gene was significantly increased compared to the RCd without antibiotics. The most significant increase was observed after the application of ampicillin (Figure 5A(a)). The highest expression of the zntR gene was observed in the RPb strain without any addition of antibiotics. After the addition of antibiotics, the intensity of the expression was reduced (Figure 5A(a)). The expression of the 16S gene (always present in bacteria) confirmed the presence of bacteria strain in the samples. This presence was independent of both on applied metal and antibiotics; therefore, the fluorescence intensity was constant (Figure 5A(b)).

Figure 5. Electrochemical analysis of the non-resistant S. aureus or RCd or RPb: (A) gene expression with and without the application of antibiotics, (a) expression of zntR gene, (b) expression of 16S gene. (B) Determination of the amount of metallothionein (MT) in (a) S. aureus, RCd or RPb; and (b) S. aureus, RCd or RPb after application of 50 µM concentration of antibiotics; (c) comparison of the values of metallothionein in strains without application and after application of antibiotics. Presented values for ATBs are the sum of individual values for every antibiotic. All data represent mean ± s.d. NS, not significant, * p < 0.05, (C) Comparison of applied concentration of heavy metal ions (950 µM) with measured concentration using atomic absorption spectrometry.

Figure 5. Electrochemical analysis of the non-resistant S. aureus or RCd or RPb: (A) gene expression with and without the application of antibiotics, (a) expression of zntR gene, (b) expression of 16S gene. (B) Determination of the amount of metallothionein (MT) in (a) S. aureus, RCd or RPb; and (b) S. aureus, RCd or RPb after application of 50 µM concentration of antibiotics; (c) comparison of the values of metallothionein in strains without application and after application of antibiotics. Presented values for ATBs are the sum of individual values for every antibiotic. All data represent mean ± s.d. NS, not significant, * p < 0.05, (C) Comparison of applied concentration of heavy metal ions (950 µM) with measured concentration using atomic absorption spectrometry.

The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.