Stem–Loop Structures in Iron-Regulated mRNAs of Giardia duodenalis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Parasite Cultures
2.2. Iron Effect on the G. duodenalis Growth
2.3. Reverse Transcriptase PCR (RT-PCR) Assays
2.4. Prediction of Stem–Loop Structures in G. duodenalis mRNAs
3. Results
3.1. Iron Affects the Growth and Viability of G. duodenalis
3.2. Actin-Related Protein, Cytochrome b5, and Glucosamine-6-Phosphate Deaminase Are Differentially Modulated by Iron
3.3. Presence of Stem–Loop Structures in G. duodenalis mRNAs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Plata-Guzmán, L.Y.; Arroyo, R.; León-Sicairos, N.; Canizález-Román, A.; López-Moreno, H.S.; Chávez-Ontiveros, J.; Garzón-Tiznado, J.A.; León-Sicairos, C. Stem–Loop Structures in Iron-Regulated mRNAs of Giardia duodenalis. Int. J. Environ. Res. Public Health 2023, 20, 3556. https://doi.org/10.3390/ijerph20043556
Plata-Guzmán LY, Arroyo R, León-Sicairos N, Canizález-Román A, López-Moreno HS, Chávez-Ontiveros J, Garzón-Tiznado JA, León-Sicairos C. Stem–Loop Structures in Iron-Regulated mRNAs of Giardia duodenalis. International Journal of Environmental Research and Public Health. 2023; 20(4):3556. https://doi.org/10.3390/ijerph20043556
Chicago/Turabian StylePlata-Guzmán, Laura Y., Rossana Arroyo, Nidia León-Sicairos, Adrián Canizález-Román, Héctor S. López-Moreno, Jeanett Chávez-Ontiveros, José A. Garzón-Tiznado, and Claudia León-Sicairos. 2023. "Stem–Loop Structures in Iron-Regulated mRNAs of Giardia duodenalis" International Journal of Environmental Research and Public Health 20, no. 4: 3556. https://doi.org/10.3390/ijerph20043556