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Article

Surveillance Web System and Mouthwash-Saliva qPCR for Labor Ambulatory SARS-CoV-2 Detection and Prevention

1
Laboratory of Epidemiological Risk Analysis (LANREF), Montecillo Campus, Postgraduate College, Texcoco 56230, CP, Mexico
2
Center for Research and Applied Technology in Jalisco (CIATEJ), Jalisco 44270, CP, Mexico
3
Secretary of Health-CDMX, Mexico City 06900, CP, Mexico
*
Author to whom correspondence should be addressed.
Academic Editors: Riccardo Polosa, Pietro Ferrara, Luciana Albano and Venera Tomaselli
Int. J. Environ. Res. Public Health 2022, 19(3), 1271; https://doi.org/10.3390/ijerph19031271
Received: 18 November 2021 / Revised: 15 January 2022 / Accepted: 17 January 2022 / Published: 24 January 2022
This study provides a safe and low-cost in-house protocol for RT-qPCR-based detection of SARS-CoV-2 using mouthwash–saliva self-collected specimens to achieve clinical and epidemiological surveillance in a real-time web environment applied to ambulatory populations. The in-house protocol comprises a mouthwash–saliva self-collected specimen, heat virus inactivation, and primers to target virus N-gene region and the human RPP30-gene. Aligning with 209 SARS-CoV-2 sequences confirmed specificity including the Alpha variant from the UK. Development, validation, and statistical comparison with official nasopharyngeal swabbing RT-qPCR test were conducted with 115 specimens of ambulatory volunteers. A web–mobile application platform was developed to integrate a real-time epidemiological and clinical core baseline database with mouthwash–saliva RT-qPCR testing. Nine built-in algorithms were generated for decision-making on testing, confining, monitoring, and self-reports to family, social, and work environments. Epidemiological and clinical follow-up and SARS-CoV-2 testing generated a database of 37,351 entries allowing individual decision-making for prevention. Mouthwash–saliva had higher sensitivity than nasopharyngeal swabbing in detecting asymptomatic and mild symptomatic cases with 720 viral copy number (VCN)/mL as the detection limit (Ct = 37.6). Cycling threshold and viral loading were marginally different (p = 0.057) between asymptomatic (35 Ct ± 2.8; 21,767.7 VCN/mL, range 720–77,278) and symptomatic (31.3 Ct ± 4.5; 747,294.3 VCN/mL, range 1433.6–3.08 × 106). We provided proof-of-concept evidence of effective surveillance to target asymptomatic and moderate symptomatic ambulatory individuals based on integrating a bio-safety level II laboratory, self-collected, low-risk, low-cost detection protocol, and a real-time digital monitoring system. Mouthwash–saliva was effective for SARS-CoV-2 sampling for the first time at the community level. View Full-Text
Keywords: coronavirus; monitoring; prevention; surveillance; COVID-19 coronavirus; monitoring; prevention; surveillance; COVID-19
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MDPI and ACS Style

Mora-Aguilera, G.; Martínez-Bustamante, V.; Acevedo-Sánchez, G.; Coria-Contreras, J.J.; Guzmán-Hernández, E.; Flores-Colorado, O.E.; Mendoza-Ramos, C.; Hernández-Nava, G.; Álvarez-Maya, I.; Gutiérrez-Espinosa, M.A.; Gómez-Linton, R.; Robles-Bustamante, A.C.; Gallardo-Hernández, A. Surveillance Web System and Mouthwash-Saliva qPCR for Labor Ambulatory SARS-CoV-2 Detection and Prevention. Int. J. Environ. Res. Public Health 2022, 19, 1271. https://doi.org/10.3390/ijerph19031271

AMA Style

Mora-Aguilera G, Martínez-Bustamante V, Acevedo-Sánchez G, Coria-Contreras JJ, Guzmán-Hernández E, Flores-Colorado OE, Mendoza-Ramos C, Hernández-Nava G, Álvarez-Maya I, Gutiérrez-Espinosa MA, Gómez-Linton R, Robles-Bustamante AC, Gallardo-Hernández A. Surveillance Web System and Mouthwash-Saliva qPCR for Labor Ambulatory SARS-CoV-2 Detection and Prevention. International Journal of Environmental Research and Public Health. 2022; 19(3):1271. https://doi.org/10.3390/ijerph19031271

Chicago/Turabian Style

Mora-Aguilera, Gustavo, Verónica Martínez-Bustamante, Gerardo Acevedo-Sánchez, Juan J. Coria-Contreras, Eduardo Guzmán-Hernández, Oscar E. Flores-Colorado, Coral Mendoza-Ramos, Gabriel Hernández-Nava, Ikuri Álvarez-Maya, M. Alejandra Gutiérrez-Espinosa, Raael Gómez-Linton, Ana Carolina Robles-Bustamante, and Alberto Gallardo-Hernández. 2022. "Surveillance Web System and Mouthwash-Saliva qPCR for Labor Ambulatory SARS-CoV-2 Detection and Prevention" International Journal of Environmental Research and Public Health 19, no. 3: 1271. https://doi.org/10.3390/ijerph19031271

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