Fentanyl Induces Novel Conditioned Place Preference in Adult Zebrafish, Disrupts Neurotransmitter Homeostasis, and Triggers Behavioral Changes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Zebrafish Maintenance
2.2. Chemicals
2.3. Zebrafish Intraperitoneal Injection Protocol
2.4. Behavioral Test
2.4.1. Conditional Place Preference Test
2.4.2. Shoaling Test
2.4.3. Novel Tank Test
2.4.4. Novel Object Exploration Test
2.4.5. Mirror Attack Test
2.4.6. Social Preference Test
2.4.7. T-Maze Memory Test
2.5. Determining the Neurotransmitters in the Brain
2.6. Statistical Analysis
3. Results
3.1. Fentanyl Induced Conditional Place Preference and Reduced Cohesion in the Shoaling Behavior of Zebrafish
3.2. Fentanyl Reduced Individual Zebrafish Anxiety in a Novel Tank Test
3.3. Fentanyl Reduced Individual Zebrafish Anxiety in Novel Object Exploration Test
3.4. Fentanyl Increased Aggressive Behavior of Individual Zebrafish in Mirror Attack Test
3.5. Fentanyl Reduced the Social Preferences of Individual Zebrafish in Social Preference Test
3.6. Fentanyl Impaired Learning Memory Capacity in the T-Maze Memory Test
3.7. Fentanyl Disturbs Neurotransmitter Stability in Zebrafish
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wu, Y.; Wang, A.; Fu, L.; Liu, M.; Li, K.; Chian, S.; Yao, W.; Wang, B.; Wang, J. Fentanyl Induces Novel Conditioned Place Preference in Adult Zebrafish, Disrupts Neurotransmitter Homeostasis, and Triggers Behavioral Changes. Int. J. Environ. Res. Public Health 2022, 19, 13533. https://doi.org/10.3390/ijerph192013533
Wu Y, Wang A, Fu L, Liu M, Li K, Chian S, Yao W, Wang B, Wang J. Fentanyl Induces Novel Conditioned Place Preference in Adult Zebrafish, Disrupts Neurotransmitter Homeostasis, and Triggers Behavioral Changes. International Journal of Environmental Research and Public Health. 2022; 19(20):13533. https://doi.org/10.3390/ijerph192013533
Chicago/Turabian StyleWu, Yuanzhao, Anli Wang, Lixiang Fu, Meng Liu, Kang Li, Song Chian, Weixuan Yao, Binjie Wang, and Jiye Wang. 2022. "Fentanyl Induces Novel Conditioned Place Preference in Adult Zebrafish, Disrupts Neurotransmitter Homeostasis, and Triggers Behavioral Changes" International Journal of Environmental Research and Public Health 19, no. 20: 13533. https://doi.org/10.3390/ijerph192013533