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Article

Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli

1
Department of Genetics, Physiology and Microbiology, Faculty of Biology, C. José Antonio Novais, 12, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain
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Department of Molecular Biology, Centro de Astrobiología (CSIC-INTA), Carretera de Ajalvir, km 4, Torrejón de Ardoz, 28850 Madrid, Spain
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Department of Forest Ecology and Genetics, Forest Research Centre (INIA, CSIC), Carretera de La Coruña, km 7.5, 28040 Madrid, Spain
4
Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Lennart Hjelms väg 9, 756 51 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Int. J. Environ. Res. Public Health 2022, 19(13), 7692; https://doi.org/10.3390/ijerph19137692
Received: 11 May 2022 / Revised: 19 June 2022 / Accepted: 21 June 2022 / Published: 23 June 2022
(This article belongs to the Special Issue New Advances in Heavy Metal Pollution)
Phytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5α, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation. View Full-Text
Keywords: phytochelatins; heavy metal resistance; stress response; oxidative stress; bioaccumulation phytochelatins; heavy metal resistance; stress response; oxidative stress; bioaccumulation
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MDPI and ACS Style

Díaz, S.; Aguilera, Á.; de Figueras, C.G.; de Francisco, P.; Olsson, S.; Puente-Sánchez, F.; González-Pastor, J.E. Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli. Int. J. Environ. Res. Public Health 2022, 19, 7692. https://doi.org/10.3390/ijerph19137692

AMA Style

Díaz S, Aguilera Á, de Figueras CG, de Francisco P, Olsson S, Puente-Sánchez F, González-Pastor JE. Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli. International Journal of Environmental Research and Public Health. 2022; 19(13):7692. https://doi.org/10.3390/ijerph19137692

Chicago/Turabian Style

Díaz, Silvia, Ángeles Aguilera, Carolina G. de Figueras, Patricia de Francisco, Sanna Olsson, Fernando Puente-Sánchez, and José E. González-Pastor. 2022. "Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli" International Journal of Environmental Research and Public Health 19, no. 13: 7692. https://doi.org/10.3390/ijerph19137692

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