Examining Wing Length–Abundance Relationships and Pyrethroid Resistance Mutations among Aedes albopictus in a Rapidly Growing Urban Area with Implications for Mosquito Surveillance and Control
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Site
2.2. DNA Extraction, Amplification, and Sequencing
2.3. Wing Length Measurements and Statistical Tests
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
kdr domain II | Forward | 5’-GACAATGTGGATCGCTTCCC-3’ | Kasai et al., 2011 [44] |
Reverse | 5’-GCAATCTGGCTTGTTAACTTG-3’ | ||
kdr domain III | Forward | 5’-GAGAACTCGCCGATGAACTT-3’ | Kasai et al., 2011 [44] |
Reverse | 5’-AACAGCAGGATCATGCTCTG-3’ | ||
kdr domain IV | Forward | 5’-TCGAGAAGTACTTCGTGTCG-3’ | Kasai et al., 2011 [44] |
Reverse | 5’-AACAGCAGGATCATGCTCTG-3’ |
Appendix B
Site ID | Neighborhood Classification | kdr Domain II | kdr Domain IV |
---|---|---|---|
2 | 4 | MZ964062 | |
3 | 1 | MZ964063 | |
4 | 2 | MZ964064 | |
5 | 3 | MZ964065 | |
6 | 1 | MZ964066 | |
7 | 2 | MZ964067 | |
8 | 1 | MZ964068 | |
9 | 1 | MZ964069 | |
10 | 4 | MZ964070 | |
11 | 2 | MZ964035 | MZ964071 |
12 | 3 | MZ964072 | |
13 | 4 | MZ964073 | |
14 | 5 | MZ964074 | |
16 | 2 | MZ964036 | MZ964075 |
17 | 3 | MZ964037 | MZ964076 |
18 | 4 | MZ964077 | |
19 | 5 | MZ964078 | |
20 | 1 | MZ964038 | MZ964079 |
22 | 3 | MZ964080 | |
23 | 5 | MZ964039 | MZ964081 |
24 | 5 | MZ964040 | MZ964082 |
25 | 1 | MZ964083 | |
26 | 4 | MZ964084 | |
27 | 3 | MZ964041 | MZ964085 |
28 | 2 | MZ964057 | MZ964086 |
29 | 4 | MZ964087 | |
30 | 2 | MZ964058 | MZ964088 |
31 | 3 | MZ964089 | |
33 | 3 | MZ964042 | MZ964090 |
34 | 4 | MZ964091 | |
35 | 3 | MZ964092 | |
37 | 1 | MZ964093 | |
38 | 1 | MZ964094 | |
40 | 4 | MZ964043 | |
41 | 5 | MZ964056 | MZ964095 |
42 | 4 | MZ964044 | MZ964096 |
43 | 5 | MZ964097 | |
44 | 3 | MZ964045 | MZ964098 |
45 | 4 | MZ964099 | |
47 | 3 | MZ964100 | |
48 | 3 | MZ964101 | |
50 | 5 | MZ964046 | MZ964102 |
51 | 5 | MZ964047 | |
52 | 5 | MZ964103 | |
53 | 1 | MZ964048 | MZ964104 |
54 | 3 | MZ964049 | MZ964105 |
55 | 4 | MZ964106 | |
56 | 3 | MZ964107 | |
57 | 2 | MZ964050 | MZ964108 |
58 | 4 | MZ964109 | |
59 | 4 | MZ964110 | |
61 | 5 | MZ964051 | MZ964111 |
62 | 2 | MZ964052 | MZ964112 |
63 | 1 | MZ964113 | |
64 | 3 | MZ964114 | |
65 | 2 | MZ964115 | |
66 | 2 | MZ964053 | MZ964116 |
67 | 3 | MZ964117 | |
68 | 5 | MZ964118 | |
69 | 2 | MZ964054 | MZ964119 |
71 | 1 | MZ964120 | |
72 | 5 | MZ964121 | |
73 | 2 | MZ964122 | |
75 | 3 | MZ964123 | |
76 | 1 | MZ964124 | |
77 | 4 | MZ964125 | |
78 | 1 | MZ964055 | MZ964126 |
79 | 5 | MZ964127 | |
80 | 4 | MZ964059 | MZ964128 |
81 | 4 | MZ964129 | |
82 | 5 | MZ964130 | |
83 | 1 | MZ964131 | |
85 | 1 | MZ964132 | |
86 | 5 | MZ964133 | |
87 | 4 | MZ964134 | |
88 | 1 | MZ964060 | |
89 | 5 | MZ964061 | MZ964135 |
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Mundis, S.J.; Hamerlinck, G.; Stone, E.K.; Whiteman, A.; Delmelle, E.; Rapp, T.; Dulin, M.; Ryan, S.J. Examining Wing Length–Abundance Relationships and Pyrethroid Resistance Mutations among Aedes albopictus in a Rapidly Growing Urban Area with Implications for Mosquito Surveillance and Control. Int. J. Environ. Res. Public Health 2021, 18, 9443. https://doi.org/10.3390/ijerph18189443
Mundis SJ, Hamerlinck G, Stone EK, Whiteman A, Delmelle E, Rapp T, Dulin M, Ryan SJ. Examining Wing Length–Abundance Relationships and Pyrethroid Resistance Mutations among Aedes albopictus in a Rapidly Growing Urban Area with Implications for Mosquito Surveillance and Control. International Journal of Environmental Research and Public Health. 2021; 18(18):9443. https://doi.org/10.3390/ijerph18189443
Chicago/Turabian StyleMundis, Stephanie J., Gabriela Hamerlinck, Emily K. Stone, Ari Whiteman, Eric Delmelle, Tyler Rapp, Michael Dulin, and Sadie J. Ryan. 2021. "Examining Wing Length–Abundance Relationships and Pyrethroid Resistance Mutations among Aedes albopictus in a Rapidly Growing Urban Area with Implications for Mosquito Surveillance and Control" International Journal of Environmental Research and Public Health 18, no. 18: 9443. https://doi.org/10.3390/ijerph18189443