Characterization and Transcriptome Analysis of a Long-Chain n-Alkane-Degrading Strain Acinetobacter pittii SW-1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strain, Media, and Chemicals
2.2. 16S rRNA Gene Analysis and Phylogenic Tree Construction
2.3. Genome Sequencing and Comparative Genomics Analysis
2.4. Growth and Biodegradation Rate of SW-1 in the Presence of Various n-Alkanes
2.5. RNA-Seq and Data Analysis
2.6. RT-qPCR
2.7. Effect of Rhamnolipid, NaCl, pH, and Temperature on C20 Utilization by Strain SW-1
2.8. Heavy Metal Tolerance Assays
2.9. Determination of the Surface Tension and Cell Surface Hydrophobicity
3. Results
3.1. Isolation and Identification of A. pittii Strain SW-1
3.2. A. pittii SW-1 Degrades LC n-Alkanes
3.3. Analysis of the Genes Involved in Alkane Degradation
3.4. Alkane Degradation-Related Genes Were Induced by C20
3.5. Detection of the Expression of Alkane Degradation-Related Genes by RT-qPCR
3.6. Effects of Rhamnolipids, NaCl, pH, Low Temperature, and Heavy Metals on A. pittii SW-1 Growth
3.7. Cell Surface Hydrophobicity Might Contribute to the Alkane Uptake in A. pittii SW-1
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain Name | Gene Name | Responsible for Range of Alkanes | Amino Acid Identity (%) | References |
---|---|---|---|---|
Acinetobacter M-1 | alkMa | C22–C30 | 86.27 % | [33] |
Acinetobacter M-1 | alkMb | C16–C22 | 60.79 % | [33] |
Acinetobacter DR1 | alkB1 | C24–C26 | 95.58 % | [17] |
Acinetobacter DR1 | alkB2 | C12–C16 | 61.04 % | [17] |
Acinetobacter ADP1 | alkM | C12–C18 | 83.09 % | [11] |
Acinetobacter RAG-1 | alkMa | C12 | 61.04 % | [34] |
Acinetobacter RAG-1 | alkMb | C12 | 86.42 % | [34] |
Acinetobacter DSM 17,874 | almA | C32 and longer | 81.49 % | [18] |
Alcanivorax Hongdengensis A-11-3 | almA | C18–C36 | 50.20 % | [35] |
P. aeruginosa SJTD-1 | almA | C18–C24 | 48.50 % | [20] |
G. thermodenitrificans NG80-2 | ladA | C15–C36 | 45.44% (with ladA1) 49.78% (with ladA2) | [19] |
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Kong, W.; Zhao, C.; Gao, X.; Wang, L.; Tian, Q.; Liu, Y.; Xue, S.; Han, Z.; Chen, F.; Wang, S. Characterization and Transcriptome Analysis of a Long-Chain n-Alkane-Degrading Strain Acinetobacter pittii SW-1. Int. J. Environ. Res. Public Health 2021, 18, 6365. https://doi.org/10.3390/ijerph18126365
Kong W, Zhao C, Gao X, Wang L, Tian Q, Liu Y, Xue S, Han Z, Chen F, Wang S. Characterization and Transcriptome Analysis of a Long-Chain n-Alkane-Degrading Strain Acinetobacter pittii SW-1. International Journal of Environmental Research and Public Health. 2021; 18(12):6365. https://doi.org/10.3390/ijerph18126365
Chicago/Turabian StyleKong, Weina, Cheng Zhao, Xingwang Gao, Liping Wang, Qianqian Tian, Yu Liu, Shuwen Xue, Zhuang Han, Fulin Chen, and Shiwei Wang. 2021. "Characterization and Transcriptome Analysis of a Long-Chain n-Alkane-Degrading Strain Acinetobacter pittii SW-1" International Journal of Environmental Research and Public Health 18, no. 12: 6365. https://doi.org/10.3390/ijerph18126365