Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals & Hormone Treatment
2.2. Measurement of Serum Hormone Levels
2.3. Determination of the Number of Implantation Sites
2.4. Transmission Electron Microscopy (TEM)
2.5. Protein Distribution Analysis by Immunohistochemistry (IHC)
2.6. Protein Quantification by Western Blotting
2.7. Statistical Analysis
3. Results
3.1. Plasma Levels of Estrogen, Progesterone and Testosterone in Pregnant Rats
3.2. Number of embryo implantation sites
3.3. TEM Images of Pinopodes
3.4. MECA-79 Protein Expression and Distribution
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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HORMONES | GROUPS | PLASMA LEVELS |
---|---|---|
ESTROGEN | C | 49.65 ± 3.66 pmol/L |
T250 | *83.35 ± 2.91 pmol/L | |
T500 | *102.67 ± 1.76 pmol/L | |
PROGESTERONE | C | 166.93 ± 8.71 nmol/L |
T250 | 134.8 ± 12.20 nmol/L | |
T500 | 161.73 ± 3.98 nmol/L | |
TESTOSTERONE | C | 0.60 ± 0.2 nmol/L |
T250 | *5.90 ± 1.44 nmol/L | |
T500 | *30.07 ± 1.47 nmol/L |
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Mokhtar, M.H.; Giribabu, N.; Salleh, N. Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats. Int. J. Environ. Res. Public Health 2020, 17, 2293. https://doi.org/10.3390/ijerph17072293
Mokhtar MH, Giribabu N, Salleh N. Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats. International Journal of Environmental Research and Public Health. 2020; 17(7):2293. https://doi.org/10.3390/ijerph17072293
Chicago/Turabian StyleMokhtar, Mohd Helmy, Nelli Giribabu, and Naguib Salleh. 2020. "Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats" International Journal of Environmental Research and Public Health 17, no. 7: 2293. https://doi.org/10.3390/ijerph17072293