Distribution of HPV Genotypes in Patients with a Diagnosis of Anal Cancer in an Italian Region
Abstract
:1. Introduction
2. Methods
2.1. Histopathological Evaluation
2.2. p16 Immunohistochemistry
2.3. HPV DNA Detection and Typing
2.4. E6-transcript Detection
2.5. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variables | N (%) | HPV-Positivity | HPV-Negativity | p-Value | |
---|---|---|---|---|---|
Gender | Male, n (%) | 16 (53.3) | 10 (47.6) | 6 (66.7) | 0.44 |
Female, n (%) | 14 (46.7) | 11 (52.4) | 3 (33.3) | ||
Mean age (SD), years | 64.2 (12.3) | 63.7 (12.2) | 65.4 (13.1) | 0.72 | |
Age ≥55 years, n (%) | 22 (73.3) | 15 (71.4) | 7 (77.8) | 1.00 | |
Civil status, n (%) * | Married | 18 (78.3) | 11 (73.39 | 7 (87.5) | 1.00 |
Single | 4 (17.4) | 3 (20.0) | 1 (12.5) | ||
Divorced | 1 (4.4) | 1 (6.7) | 0 (0.0) | ||
Occupational status, n (%) * | Unemployed | 7 (35.0) | 6 (46.2) | 1 (14.3) | 0.43 |
Employed | 5 (25.0) | 3 (23.1) | 2 (28.6) | ||
Retired | 8 (40.0) | 4 (30.8) | 4 (57.1) | ||
Suspected clinical diagnosis of carcinoma, n (%) | 28 (96.6) | 20 (95.2) | 8 (100.0) | 1.00 | |
Suspected clinical diagnosis of adenoma, n (%) | 1 (3.5) | 1 (4.8) | 0 (0.0) | 1.00 | |
Suspected clinical diagnosis of condyloma, n (%) | 3 (10.3) | 2 (9.5) | 1 (12.5) | 1.00 | |
Cancer diagnosed before 2010, n (%) | 19 (63.3) | 13 (61.9) | 6 (66.7) | 1.00 | |
Histopathological results, n (%) | Adenocarcinoma | 11 (36.7) | 7 (30.4) | 4 (40.0) | 0.69 |
Squamous Cell Carcinoma | 19 (63.3) | 14 (66.7) | 5 (55.6) | ||
Histopathological grading (AJCC), n (%) | G1 | 12 (40.0) | 10 (47.6) | 2 (22.2) | 0.25 |
G2 | 13 (43.3) | 6 (28.6) | 7 (77.8) | 0.02 | |
G3 | 5 (16.7) | 5 (23.8) | 0 (0.0) | 0.29 | |
HPV-DNA positivity, n (%) | 21 (70.0) | 21 (100.0) | 0 (0.0) | - | |
HPV-16 | 18 (60.0) | 18 (85.7) | 0 (0.0) | - | |
HPV-35 | 2 (6.7) | 2 (9.5) | 0 (0.0) | - | |
HPV-6 | 3 (10.0) | 3 (14.3) | 0 (0.0) | - | |
p-16 immunohistochemistry positivity, n (%) | 12 (40.0) | 12 (57.1) | 0 (0.0) | 0.004 | |
E6 gene positivity, n (%) | 5 (33.3) | 5 (100.0) | 0 (0.0) | - | |
Comorbidity, n (%) * | Colic adenoma | 6 (66.7) | 5 (71.4) | 1 (71.4) | 0.25 |
Bladder carcinoma | 2 (22.2) | 2 (28.6) | 0 (0.0) | ||
Others | 1 (11.1) | 0 (0.0) | 1 (50.0) | ||
Mortality, n (%) | 14 (46.7) | 10 (47.6) | 4 (44.4) | 1.00 | |
Five-year mortality, n (%) | 12 (40.0) | 10 (47.6) | 2 (22.2) | 0.25 |
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Muresu, N.; Sotgiu, G.; Saderi, L.; Sechi, I.; Cossu, A.; Marras, V.; Meloni, M.; Martinelli, M.; Cocuzza, C.; Tanda, F.; et al. Distribution of HPV Genotypes in Patients with a Diagnosis of Anal Cancer in an Italian Region. Int. J. Environ. Res. Public Health 2020, 17, 4516. https://doi.org/10.3390/ijerph17124516
Muresu N, Sotgiu G, Saderi L, Sechi I, Cossu A, Marras V, Meloni M, Martinelli M, Cocuzza C, Tanda F, et al. Distribution of HPV Genotypes in Patients with a Diagnosis of Anal Cancer in an Italian Region. International Journal of Environmental Research and Public Health. 2020; 17(12):4516. https://doi.org/10.3390/ijerph17124516
Chicago/Turabian StyleMuresu, Narcisa, Giovanni Sotgiu, Laura Saderi, Illari Sechi, Antonio Cossu, Vincenzo Marras, Marta Meloni, Marianna Martinelli, Clementina Cocuzza, Francesco Tanda, and et al. 2020. "Distribution of HPV Genotypes in Patients with a Diagnosis of Anal Cancer in an Italian Region" International Journal of Environmental Research and Public Health 17, no. 12: 4516. https://doi.org/10.3390/ijerph17124516