Next Article in Journal
Spectral Feature Selection Optimization for Water Quality Estimation
Previous Article in Journal
Erratum: Florida Arsenic Distribution Index: Quantifying the Distribution of Past and Present Arsenic Usage
Article

Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment

1
Department of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, 00185 Rome, Italy
2
Department of Chemistry, Sapienza University of Rome, 00185 Rome, Italy
3
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy
4
Inail DIT, 00143 Rome, Italy
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2020, 17(1), 273; https://doi.org/10.3390/ijerph17010273
Received: 15 November 2019 / Revised: 25 December 2019 / Accepted: 26 December 2019 / Published: 30 December 2019
Wastewater carries different pathogenic and non-pathogenic microorganisms that can be dispersed in the surrounding environment. Workers who frequent sewage treatment plants can therefore be exposed to aerosols that contain a high concentration of potentially dangerous biological agents, or they can come into direct contact with contaminated material. This can lead to allergies, infections and occupational health-associated diseases. A characterization of biological risk assessment of bioaerosol exposure is necessary. The aim of this study was to evaluate the application of an interdisciplinary method that combines chemical and biological approaches for the analysis of a bioaerosol derived from a wastewater treatment plant (WWTP) situated in Italy. Sampled filters were analyzed by HPLC-MS/MS spectroscopy that searched for different chemical biomarkers of airborne microorganisms. The analytical quantification was compared to the biological cultural method that revealed an underrated microbial concentration. Furthermore, next generation sequencing analysis was used also to identify the uncultivable species that were not detected by the culture dependent-method. Moreover, the simple animal model Caenorhabditis elegans was used to evaluate the pathogenicity of two isolates—Acinetobacter iwoffii and Micrococcus luteus—that showed multidrug-resistance. This work represents a starting point for the development of a multidisciplinary approach for the validation of bioaerosol exposure on WWTP workplaces. View Full-Text
Keywords: airborne; Caenorhabditis elegans; worker exposure; bacterial bioaerosol; wastewater airborne; Caenorhabditis elegans; worker exposure; bacterial bioaerosol; wastewater
Show Figures

Figure 1

MDPI and ACS Style

Bruni, E.; Simonetti, G.; Bovone, B.; Casagrande, C.; Castellani, F.; Riccardi, C.; Pomata, D.; Di Filippo, P.; Federici, E.; Buiarelli, F.; Uccelletti, D. Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment. Int. J. Environ. Res. Public Health 2020, 17, 273. https://doi.org/10.3390/ijerph17010273

AMA Style

Bruni E, Simonetti G, Bovone B, Casagrande C, Castellani F, Riccardi C, Pomata D, Di Filippo P, Federici E, Buiarelli F, Uccelletti D. Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment. International Journal of Environmental Research and Public Health. 2020; 17(1):273. https://doi.org/10.3390/ijerph17010273

Chicago/Turabian Style

Bruni, Erika, Giulia Simonetti, Beatrice Bovone, Chiara Casagrande, Federica Castellani, Carmela Riccardi, Donatella Pomata, Patrizia Di Filippo, Ermanno Federici, Francesca Buiarelli, and Daniela Uccelletti. 2020. "Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment" International Journal of Environmental Research and Public Health 17, no. 1: 273. https://doi.org/10.3390/ijerph17010273

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop