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Open AccessArticle

Sulforaphane Cannot Protect Human Fibroblasts From Repeated, Short and Sublethal Treatments with Hydrogen Peroxide

1
Center for Complexity and Biosystems, Department of Environmental Science and Policy, University of Milan, via Celoria 26, 20133 Milano, Italy
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Center for Complexity and Biosystems, Department of Physics, University of Milan, via Celoria 16, 20133 Milano, Italy
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Department of Biomedical, Surgical and Dental Sciences, University of Milan, Via Pascal 36, 20133 Milano, Italy
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Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy
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Department of Environmental Science and Policy, University of Milan, via Celoria 10, 20133 Milano, Italy
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SIMA, Societá Italiana di Medicina Ambientale, via Monte Leone 2, 20149 Milano, Italy
7
Fondazione IRCCS Istituto Neurologico C. Besta, Via Celoria 11, 20133 Milano, Italy
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2019, 16(4), 657; https://doi.org/10.3390/ijerph16040657
Received: 13 January 2019 / Revised: 17 February 2019 / Accepted: 18 February 2019 / Published: 23 February 2019
(This article belongs to the Section Environmental Health)
A delicate balance of reactive oxygen species (ROS) exists inside the cell: when the mechanisms that control the level of ROS fail, the cell is in an oxidative stress state, a condition that can accelerate aging processes. To contrast the pro-aging effect of ROS, the supplementation of antioxidants has been recently proposed. Sulforaphane (SFN) is an isothiocyanate isolated from Brassica plants that has been shown to modulate many critical factors inside the cells helping to counteract aging processes. In the present work, we exposed human dermal fibroblast to short, sublethal and repeated treatments with hydrogen peroxide for eight days, without or in combination with low concentration of SFN. Hydrogen peroxide treatments did not affect the oxidative status of the cells, without any significant change of the intracellular ROS levels or the number of mitochondria or thiols in total proteins. However, our regime promoted cell cycle progression and cell viability, increased the anti-apoptotic factor survivin and increased DNA damage, measured as number of foci positive for γ -H2AX. On the other hand, the treatment with SFN alone seemed to exert a protective effect, increasing the level of p53, which can block the expansion of possible DNA damaged cells. However, continued exposure to SFN at this concentration could not protect the cells from stress induced by hydrogen peroxide. View Full-Text
Keywords: oxidative stress; sulforaphane; fibrobalsts; p53 oxidative stress; sulforaphane; fibrobalsts; p53
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Lionetti, M.C.; Mutti, F.; Soldati, E.; Fumagalli, M.R.; Coccé, V.; Colombo, G.; Astori, E.; Miani, A.; Milzani, A.; Dalle-Donne, I.; Ciusani, E.; Costantini, G.; La Porta, C.A.M. Sulforaphane Cannot Protect Human Fibroblasts From Repeated, Short and Sublethal Treatments with Hydrogen Peroxide. Int. J. Environ. Res. Public Health 2019, 16, 657.

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