1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultureand Ethics Statement
2.2. RF-EMR Exposure Equipment and Exposure Parameter
2.3. Cell Proliferation Assay
2.4. Cell Cycle Analysis
2.5. Cell Apoptosis Analysis
2.6. Testosterone Content Assay
2.7. StAR and P450scc mRNA Expression
2.8. Intracellular Cell ROS Analysis
2.9. Statistical Analysis
3. Results
3.1. Proliferation of TM3 Cell was Inhibited by RF Radiation
3.2. RF Radiation Resulted in S Phase Arrest
3.3. RF Radiation Produced No Effects on TM3 Cell Apoptosis
3.4. Testosterone Secretion Inhibited by RF Radiation
3.5. StAR and P450scc mRNA Expression Downregulated by RF Radiation
3.6. RF Radiation Produced No Effect on Intracellular ROS Generation
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Days | G1 | S | G2 | |||
---|---|---|---|---|---|---|
Sham | Exposure | Sham | Exposure | Sham | Exposure | |
1 | 45.56 ± 1.22 | 43.60 ± 1.54 | 26.75 ± 1.26 | 31.66 ± 1.00 ** | 27.69 ± 2.41 | 24.74 ± 2.19 |
2 | 51.16 ± 0.15 | 50.73 ± 2.41 | 20.18 ± 1.10 | 23.76 ± 0.47 ** | 28.66 ± 2.02 | 25.51 ± 2.46 |
3 | 51.44 ± 0.81 | 42.36 ± 3.77 * | 27.49 ± 2.40 | 35.20 ± 2.28 * | 21.07 ± 1.56 | 22.44 ± 1.1 |
4 | 49.46 ± 3.44 | 45.38 ± 1.44 | 25.62 ± 0.45 | 27.63 ± 1.06 * | 24.92 ± 0.96 | 26.99 ± 1.04 |
5 | 55.38 ± 3.15 | 47.80 ± 0.84 * | 20.14 ± 0.49 | 29.88 ± 2.53 ** | 24.21 ± 1.45 | 22.32 ± 0.83 |
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Lin, Y.-Y.; Wu, T.; Liu, J.-Y.; Gao, P.; Li, K.-C.; Guo, Q.-Y.; Yuan, M.; Lang, H.-Y.; Zeng, L.-H.; Guo, G.-Z. 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells. Int. J. Environ. Res. Public Health 2018, 15, 17. https://doi.org/10.3390/ijerph15010017
Lin Y-Y, Wu T, Liu J-Y, Gao P, Li K-C, Guo Q-Y, Yuan M, Lang H-Y, Zeng L-H, Guo G-Z. 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells. International Journal of Environmental Research and Public Health. 2018; 15(1):17. https://doi.org/10.3390/ijerph15010017
Chicago/Turabian StyleLin, Yan-Yun, Tao Wu, Jun-Ye Liu, Peng Gao, Kang-Chu Li, Qi-Yan Guo, Meng Yuan, Hai-Yang Lang, Li-Hua Zeng, and Guo-Zhen Guo. 2018. "1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells" International Journal of Environmental Research and Public Health 15, no. 1: 17. https://doi.org/10.3390/ijerph15010017
APA StyleLin, Y.-Y., Wu, T., Liu, J.-Y., Gao, P., Li, K.-C., Guo, Q.-Y., Yuan, M., Lang, H.-Y., Zeng, L.-H., & Guo, G.-Z. (2018). 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells. International Journal of Environmental Research and Public Health, 15(1), 17. https://doi.org/10.3390/ijerph15010017