Next Article in Journal
Efficacy of a Low Dose of Hydrogen Peroxide (Peroxy Ag+) for Continuous Treatment of Dental Unit Water Lines: Challenge Test with Legionella pneumophila Serogroup 1 in a Simulated Dental Unit Waterline
Previous Article in Journal
Environmental Risk Factors in Psoriasis: The Point of View of the Nutritionist
Article Menu

Export Article

Open AccessArticle
Int. J. Environ. Res. Public Health 2016, 13(7), 742;

DNA Damage in Euonymus japonicus Leaf Cells Caused by Roadside Pollution in Beijing

School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China
School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
Brook Byers Institute for Sustainable Systems, Georgia Institute of Technology, 828 West Peachtree St., Suite 320B, Atlanta, GA 30332, USA
Author to whom correspondence should be addressed.
Academic Editor: William A. Toscano
Received: 3 March 2016 / Revised: 12 June 2016 / Accepted: 24 June 2016 / Published: 22 July 2016
Full-Text   |   PDF [1866 KB, uploaded 22 July 2016]   |  


The inhalable particles from vehicle exhaust can cause DNA damage to exposed organisms. Research on DNA damage is primarily focused on the influence of specific pollutants on certain species or the effect of environmental pollution on human beings. To date, little research has quantitatively studied the relationship between roadside pollution and DNA damage. Based on an investigation of the roadside pollution in Beijing, Euonymus japonicus leaves of differing ages grown in heavily-polluted sections were chosen as biomonitors to detect DNA damage using the comet assay technique. The percentage of DNA in the tail and tail moment was chosen as the analysis index based on SPSS data analysis. The roadside samples showed significantly higher levels of DNA damage than non-roadside samples, which increased in older leaves, and the DNA damage to Euonymus japonicus leaf cells was positively correlated with haze-aggravated roadside pollution. The correlation between damage and the Air Quality Index (AQI) are 0.921 (one-year-old leaves), 0.894 (two-year-old leaves), and 0.878 (three-year-old leaves). Over time, the connection between DNA damage and AQI weakened, with the sensitivity coefficient for δyear 1 being larger than δyear 2 and δyear 3. These findings support the suitability and sensitivity of the comet assay for surveying plants for an estimation of DNA damage induced by environmental genotoxic agents. This study might be applied as a preliminary quantitative method for Chinese urban air pollution damage assessment caused by environmental stress. View Full-Text
Keywords: comet assay; percentage of DNA in the tail; tail moment; air pollution comet assay; percentage of DNA in the tail; tail moment; air pollution

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Li, T.; Zhang, M.; Gu, K.; Herman, U.; Crittenden, J.; Lu, Z. DNA Damage in Euonymus japonicus Leaf Cells Caused by Roadside Pollution in Beijing. Int. J. Environ. Res. Public Health 2016, 13, 742.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top