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Open AccessArticle

Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes

Department of Hygiene and Public Health, Nippon Medical School, Tokyo 113-8602 Japan
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Academic Editor: Paul B. Tchounwou
Int. J. Environ. Res. Public Health 2015, 12(4), 3633-3645; https://doi.org/10.3390/ijerph120403633
Received: 13 February 2015 / Revised: 18 March 2015 / Accepted: 25 March 2015 / Published: 1 April 2015
We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram > ziram > maneb > carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c. View Full-Text
Keywords: annexin-V; apoptosis; carbamate pesticide; carbaryl; caspase; cytochrome-c; Jurkat T cell; maneb; thiram; ziram annexin-V; apoptosis; carbamate pesticide; carbaryl; caspase; cytochrome-c; Jurkat T cell; maneb; thiram; ziram
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Li, Q.; Kobayashi, M.; Kawada, T. Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes. Int. J. Environ. Res. Public Health 2015, 12, 3633-3645.

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