Next Article in Journal
Recess Physical Activity and Perceived School Environment among Elementary School Children
Previous Article in Journal
Correction: Brewer, R.; et al. Risk-Based Evaluation of Total Petroleum Hydrocarbons in Vapor Intrusion Studies. Int. J. Environ. Res. Public Health 2013, 10, 2441–2467
Article

Inactivation of Selected Bacterial Pathogens in Dairy Cattle Manure by Mesophilic Anaerobic Digestion (Balloon Type Digester)

1
Fort Hare Institute of Technology, University of Fort Hare, Alice Campus, Alice 5700, Eastern Cape Province, South Africa
2
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice Campus, Alice 5700, Eastern Cape Province, South Africa
3
Department of Physics, University of Fort Hare, Alice Campus, Alice 5700, Eastern Cape Province, South Africa
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2014, 11(7), 7184-7194; https://doi.org/10.3390/ijerph110707184
Received: 21 May 2014 / Revised: 4 July 2014 / Accepted: 7 July 2014 / Published: 14 July 2014
Anaerobic digestion of animal manure in biogas digesters has shown promise as a technology in reducing the microbial load to safe and recommended levels. We sought to treat dairy manure obtained from the Fort Hare Dairy Farm by investigating the survival rates of bacterial pathogens, through a total viable plate count method, before, during and after mesophilic anaerobic digestion. Different microbiological media were inoculated with different serial dilutions of manure samples that were withdrawn from the biogas digester at 3, 7 and 14 day intervals to determine the viable cells. Data obtained indicated that the pathogens of public health importance were 90%–99% reduced in the order: Campylobacter sp. (18 days) < Escherichia coli sp. (62 days) < Salmonella sp. (133 days) from a viable count of 10.1 × 103, 3.6 × 105, 7.4 × 103 to concentrations below the detection limit (DL = 102 cfu/g manure), respectively. This disparity in survival rates may be influenced by the inherent characteristics of these bacteria, available nutrients as well as the stages of the anaerobic digestion process. In addition, the highest p-value i.e., 0.957 for E. coli showed the statistical significance of its model and the strongest correlation between its reductions with days of digestion. In conclusion, the results demonstrated that the specific bacterial pathogens in manure can be considerably reduced through anaerobic digestion after 133 days. View Full-Text
Keywords: anaerobic digestion; dairy manure; enteropathogens; viable counts; regression models; South Africa anaerobic digestion; dairy manure; enteropathogens; viable counts; regression models; South Africa
Show Figures

Figure 1

MDPI and ACS Style

Manyi-Loh, C.E.; Mamphweli, S.N.; Meyer, E.L.; Okoh, A.I.; Makaka, G.; Simon, M. Inactivation of Selected Bacterial Pathogens in Dairy Cattle Manure by Mesophilic Anaerobic Digestion (Balloon Type Digester). Int. J. Environ. Res. Public Health 2014, 11, 7184-7194. https://doi.org/10.3390/ijerph110707184

AMA Style

Manyi-Loh CE, Mamphweli SN, Meyer EL, Okoh AI, Makaka G, Simon M. Inactivation of Selected Bacterial Pathogens in Dairy Cattle Manure by Mesophilic Anaerobic Digestion (Balloon Type Digester). International Journal of Environmental Research and Public Health. 2014; 11(7):7184-7194. https://doi.org/10.3390/ijerph110707184

Chicago/Turabian Style

Manyi-Loh, Christy E., Sampson N. Mamphweli, Edson L. Meyer, Anthony I. Okoh, Golden Makaka, and Michael Simon. 2014. "Inactivation of Selected Bacterial Pathogens in Dairy Cattle Manure by Mesophilic Anaerobic Digestion (Balloon Type Digester)" International Journal of Environmental Research and Public Health 11, no. 7: 7184-7194. https://doi.org/10.3390/ijerph110707184

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop