In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of Neorogioltriol on Carrageenan-Induced Paw Edema in Rats
2.2. Cell Viability
2.3. Effect of Neorogioltriol on TNFα Release, NO Production and COX-2 Expression in LPS-Stimulated Raw264.7 Cells
2.4. Effects of Neorogioltriol on NF-κB Transactivation in LPS-Stimulated Raw264.7 Cells
2.5. Effect of Neorogioltriol on MAPK in LPS-Stimulated Raw264.7 Cells
3. Experimental Section
3.1. Isolation of Neorogioltriol
3.2. Carrageenan-Induced Paw Edema
3.3. Reagents and Antibodies
3.4. Cells Culture
3.5. Cytotoxicity Test
3.6. Transfection and Luciferase Assay
3.7. Western Blot Analysis
3.8. Measurements of Nitrite Production and Cytokine Assays
3.9. Statistical Analysis
4. Conclusions
Acknowledgements
- Samples Availability: Available from the authors.
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Times after carageenan injection (h) | 1 h | 3 h | 5 h | ||||||
---|---|---|---|---|---|---|---|---|---|
Groups | Treatment | n | Dose (mg/kg) | EV | EI | EV | EI | EV | EI |
(10−2 mL) | (%) | (10−2 mL) | (%) | (10−2 mL) | (%) | ||||
Control | Control | 6 | - | 18.3 ± 2.7 | - | 56.3 ± 5.8 | - | 69 ± 7.7 | - |
Tests | Neorogioltriol | 6 | 0.5 | 15.5 ± 3.1 | 15.3 | 42 ± 5.5 (*) | 33.3 | 49.1 ± 6.1 | 28.8 |
1 | 14.1 ± 3 (*) | 22.95 | 23.5 ± 3.7 (***) | 58 | 33 ± 3.5 (**) | 52.27 | |||
References | Aspirin (ASA) | 6 | 300 | 16.5 ± 3.1 | 9.83 | 25.1 ± 6.7 (**) | 55.17 | 28.1 ± 2.2 (**) | 59.27 |
Dexamethasone | 6 | 1 | 9.83 ± 1.8 | 47.54 | 39.3 ± 3.8 (***) | 42.5 | 32.5 ± 5.7 (***) | 52.86 |
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Chatter, R.; Othman, R.B.; Rabhi, S.; Kladi, M.; Tarhouni, S.; Vagias, C.; Roussis, V.; Guizani-Tabbane, L.; Kharrat, R. In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera. Mar. Drugs 2011, 9, 1293-1306. https://doi.org/10.3390/md9071293
Chatter R, Othman RB, Rabhi S, Kladi M, Tarhouni S, Vagias C, Roussis V, Guizani-Tabbane L, Kharrat R. In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera. Marine Drugs. 2011; 9(7):1293-1306. https://doi.org/10.3390/md9071293
Chicago/Turabian StyleChatter, Rim, Rym Ben Othman, Sameh Rabhi, Maria Kladi, Safa Tarhouni, Constantinos Vagias, Vassilios Roussis, Lamia Guizani-Tabbane, and Riadh Kharrat. 2011. "In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera" Marine Drugs 9, no. 7: 1293-1306. https://doi.org/10.3390/md9071293