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Open AccessArticle

Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver

School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
Author to whom correspondence should be addressed.
Mar. Drugs 2009, 7(2), 258-267;
Received: 20 April 2009 / Revised: 13 June 2009 / Accepted: 13 June 2009 / Published: 22 June 2009
(This article belongs to the Special Issue Alkaloid Analogs)
The Active Peptide from Shark Liver (APSL) was expressed in E. coli BL21 cells. The cDNA encoding APSL protein was obtained from shark regenerated hepatic tissue by RT-PCR, then it was cloned in the pET-28a expression vector. The expressed fusion protein was purified by Ni-IDA affinity chromatography. SDS-PAGE and HPLC analysis showed the purity of the purified fusion protein was more than 98%. The recombinant APSL (rAPSL) was tested for its biological activity both in vitro, by its ability to improve the proliferation of SMMC7721 cells, and in vivo, by its significant protective effects against acute hepatic injury induced by CCl4 and AAP (acetaminophen) in mice. In addition, the rAPSL could decrease the blood glucose concentration of mice with diabetes mellitus induced by alloxan. Paraffin sections of mouse pancreas tissues showed that rAPSL (3 mg/kg) could effectively protect mouse islets from lesions induced by alloxan, which indicated its potential application in theoretical research and industry. View Full-Text
Keywords: APSL; prokaryotic expression; bioactivity analysis APSL; prokaryotic expression; bioactivity analysis
MDPI and ACS Style

Lv, Z.; Ou, Y.; Li, Q.; Zhang, W.; Ye, B.; Wu, W. Expression, Purification and Bioactivities Analysis of Recombinant Active Peptide from Shark Liver. Mar. Drugs 2009, 7, 258-267.

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