Preparation of Ion2+-COS/SA Multifunctional Gel Films for Skin Wound Healing by an In Situ Spray Method
Abstract
:1. Introduction
2. Results and Discussions
2.1. Preparation Mechanism of COS/SA/M2+ PDF
2.2. Chemical Structure and Property Analysis of COS/SA/M2+ PDF
2.2.1. Surface Appearance
2.2.2. FTIR
2.2.3. XRD
2.2.4. Contact Angle
2.2.5. Inflationary Behavior
2.2.6. Porosity
2.2.7. Thermal Stability
2.3. Mechanical and Rheological Analysis of COS/SA/M2+ PDF
2.4. Antioxidant Activity of COS/SA/M2+ PDF
2.5. Antibacterial Properties of COS/SA/M2+ PDF
2.6. Cytocompatibility of COS/SA/M2+ PDF
2.7. In Vivo Healing Promotion Experiment of COS/SA/M2+ PDF
2.7.1. Wound Healing Assessment
2.7.2. Histological Evaluation of the Regenerated Tissue
2.7.3. Expression of FGF and CD31 during Wound Healing
3. Materials and Methods
3.1. Experimental Materials
3.2. Preparation of Ion2+-COS/SA Gel Films
3.3. SEM Morphological Analysis
3.4. FTIR
3.5. XRD
3.6. Contact Angle
3.7. Mechanical and Rheological Property Testing
3.8. Inflationary Behavior
3.9. Porosity Measurements
3.10. Thermal Stability
3.11. Antioxidant Activity Analysis
3.11.1. FRAP Analysis
3.11.2. DPPH Free Radical Scavenging Activity
3.11.3. Ability to Scavenge ABTS Free Radicals
3.12. Antibacterial Activity Analysis
3.13. Cell Viability Assay
3.14. In Vivo Healing Promotion Experiment
3.14.1. Construction of the Trauma Models
3.14.2. Histological Assessment
3.14.3. Immunohistochemistry Analysis
3.15. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, L.; Guo, T.; Shi, C.; Zhang, K.; Cui, S.; Zhao, D.; Yang, F.; Chen, J. Preparation of Ion2+-COS/SA Multifunctional Gel Films for Skin Wound Healing by an In Situ Spray Method. Mar. Drugs 2022, 20, 401. https://doi.org/10.3390/md20060401
Chen L, Guo T, Shi C, Zhang K, Cui S, Zhao D, Yang F, Chen J. Preparation of Ion2+-COS/SA Multifunctional Gel Films for Skin Wound Healing by an In Situ Spray Method. Marine Drugs. 2022; 20(6):401. https://doi.org/10.3390/md20060401
Chicago/Turabian StyleChen, Liqi, Tingting Guo, Chao Shi, Kun Zhang, Shenghao Cui, Di Zhao, Faming Yang, and Jingdi Chen. 2022. "Preparation of Ion2+-COS/SA Multifunctional Gel Films for Skin Wound Healing by an In Situ Spray Method" Marine Drugs 20, no. 6: 401. https://doi.org/10.3390/md20060401