In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects

Dutot, Mélody; Olivier, Elodie; Fouyet, Sophie; Magny, Romain; Hammad, Karim; Roulland, Emmanuel; Rat, Patrice; Fagon, Roxane

doi:10.3390/md19010034

Open AccessArticle

In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects

by

Mélody Dutot

^1,2,*

,

Elodie Olivier

²,

Sophie Fouyet

²,

Romain Magny

²,

Karim Hammad

²,

Emmanuel Roulland

²

,

Patrice Rat

²

and

Roxane Fagon

¹

Recherche & Développement, Yslab, 29000 Quimper, France

²

Faculté de Pharmacie de Paris, UMR CNRS 8038, Université de Paris, 75006 Paris, France

^*

Author to whom correspondence should be addressed.

Mar. Drugs 2021, 19(1), 34; https://doi.org/10.3390/md19010034

Submission received: 8 December 2020 / Revised: 4 January 2021 / Accepted: 12 January 2021 / Published: 14 January 2021

(This article belongs to the Collection Marine Compounds and Cancer)

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Abstract

Phlorotannins are polyphenols occurring exclusively in some species of brown algae, known for numerous biological activities, e.g., antioxidant, antiproliferative, antidiabetic, and antiallergic properties. Their effects on the response of human lung cells to benzo[a]pyrene (B[a]P) has not been characterized. Our objective was to in vitro evaluate the effects of a phlorotannin-rich extract obtained from the brown algae Ascophyllum nodosum and Fucus vesiculosus on B[a]P cytotoxic effects. The A549 cell line was incubated with B[a]P for 48 and 72 h in the presence or absence of the brown algae extract. Cytochrome P450 activity, activation of P2X7 receptor, F-actin disorganization, and loss of E-cadherin expression were assessed using microplate cytometry and fluorescence microscopy. Relative to control, incubation with the brown algae extract was associated with lower B[a]P-induced CYP1 activity, lower P2X7 receptor activation, and lower reactive oxygen species production. The brown algae extract inhibited the alterations of F-actin arrangement and the downregulation of E-cadherin expression. We identified a phlorotannins-rich extract that could be deeper investigated as a cancer chemopreventive agent to block B[a]P-mediated carcinogenesis.

Keywords: A549 cells; cancer; cytoskeleton; phlorotannins; P2X7 receptor; pollution

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MDPI and ACS Style

Dutot, M.; Olivier, E.; Fouyet, S.; Magny, R.; Hammad, K.; Roulland, E.; Rat, P.; Fagon, R. In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects. Mar. Drugs 2021, 19, 34. https://doi.org/10.3390/md19010034

AMA Style

Dutot M, Olivier E, Fouyet S, Magny R, Hammad K, Roulland E, Rat P, Fagon R. In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects. Marine Drugs. 2021; 19(1):34. https://doi.org/10.3390/md19010034

Chicago/Turabian Style

Dutot, Mélody, Elodie Olivier, Sophie Fouyet, Romain Magny, Karim Hammad, Emmanuel Roulland, Patrice Rat, and Roxane Fagon. 2021. "In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects" Marine Drugs 19, no. 1: 34. https://doi.org/10.3390/md19010034

APA Style

Dutot, M., Olivier, E., Fouyet, S., Magny, R., Hammad, K., Roulland, E., Rat, P., & Fagon, R. (2021). In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects. Marine Drugs, 19(1), 34. https://doi.org/10.3390/md19010034

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In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects

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