Genome Mining, Microbial Interactions, and Molecular Networking Reveals New Dibromoalterochromides from Strains of Pseudoalteromonas of Coiba National Park-Panama

Atencio, Librada A.; Boya P., Cristopher A.; Martin H., Christian; Mejía, Luis C.; Dorrestein, Pieter C.; Gutiérrez, Marcelino

doi:10.3390/md18090456

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Genome Mining, Microbial Interactions, and Molecular Networking Reveals New Dibromoalterochromides from Strains of Pseudoalteromonas of Coiba National Park-Panama

Librada A. Atencio

^1,2

Cristopher A. Boya P.

^1,2

^1,2

^1,3,*

⁴ and

^1,*

Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT), Clayton, Panama City 0843-01103, Panama

Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, India

Smithsonian Tropical Research Institute, Balboa Ancón, Panama City 0843-03092, Panama

⁴

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA

Authors to whom correspondence should be addressed.

Mar. Drugs 2020, 18(9), 456; https://doi.org/10.3390/md18090456

Received: 14 July 2020 / Revised: 27 August 2020 / Accepted: 31 August 2020 / Published: 3 September 2020

(This article belongs to the Special Issue Molecular Networking as a Strategy in Discovery of Marine Bioactive Compounds)

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Abstract

The marine bacterial genus Pseudoalteromonas is known for their ability to produce antimicrobial compounds. The metabolite-producing capacity of Pseudoalteromonas has been associated with strain pigmentation; however, the genomic basis of their antimicrobial capacity remains to be explained. In this study, we sequenced the whole genome of six Pseudoalteromonas strains (three pigmented and three non-pigmented), with the purpose of identifying biosynthetic gene clusters (BGCs) associated to compounds we detected via microbial interactions along through MS-based molecular networking. The genomes were assembled and annotated using the SPAdes and RAST pipelines and mined for the identification of gene clusters involved in secondary metabolism using the antiSMASH database. Nineteen BGCs were detected for each non-pigmented strain, while more than thirty BGCs were found for two of the pigmented strains. Among these, the groups of genes of nonribosomal peptide synthetases (NRPS) that code for bromoalterochromides stand out the most. Our results show that all strains possess BGCs for the production of secondary metabolites, and a considerable number of distinct polyketide synthases (PKS) and NRPS clusters are present in pigmented strains. Furthermore, the molecular networking analyses revealed two new molecules produced during microbial interactions: the dibromoalterochromides D/D’ (11–12). View Full-Text

Keywords: Pseudoalteromonas; bromoalterochromides; antimicrobials; genome mining; biosynthetic gene cluster; MS/MS molecular networking; Coiba National Park

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Atencio, L.A.; Boya P., C.A.; Martin H., C.; Mejía, L.C.; Dorrestein, P.C.; Gutiérrez, M. Genome Mining, Microbial Interactions, and Molecular Networking Reveals New Dibromoalterochromides from Strains of Pseudoalteromonas of Coiba National Park-Panama. Mar. Drugs 2020, 18, 456. https://doi.org/10.3390/md18090456

AMA Style

Atencio LA, Boya P. CA, Martin H. C, Mejía LC, Dorrestein PC, Gutiérrez M. Genome Mining, Microbial Interactions, and Molecular Networking Reveals New Dibromoalterochromides from Strains of Pseudoalteromonas of Coiba National Park-Panama. Marine Drugs. 2020; 18(9):456. https://doi.org/10.3390/md18090456

Chicago/Turabian Style

Atencio, Librada A., Cristopher A. Boya P., Christian Martin H., Luis C. Mejía, Pieter C. Dorrestein, and Marcelino Gutiérrez. 2020. "Genome Mining, Microbial Interactions, and Molecular Networking Reveals New Dibromoalterochromides from Strains of Pseudoalteromonas of Coiba National Park-Panama" Marine Drugs 18, no. 9: 456. https://doi.org/10.3390/md18090456

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Genome Mining, Microbial Interactions, and Molecular Networking Reveals New Dibromoalterochromides from Strains of Pseudoalteromonas of Coiba National Park-Panama

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