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Open AccessArticle

Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography

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Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China
2
School of Marine Science, Ningbo University, Ningbo 315211, China
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State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China
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Shandong Provincial Key Laboratory of Synthetic Biology, and CAS Key Laboratory of Biofuels at Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China
5
University of Chinese Academy of Sciences, Beijing 100049, China
6
Rushan Hanwei Biological Science and Technology Co., Ltd., Rushan 264502, China
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2020, 18(6), 332; https://doi.org/10.3390/md18060332
Received: 4 June 2020 / Revised: 20 June 2020 / Accepted: 23 June 2020 / Published: 25 June 2020
An efficient strategy was developed for the rapid separation and enrichment of bafilomycin A1 (baf A1) from a crude extract of the marine microorganism Streptomyces lohii fermentation. This strategy comprises liquid−liquid extraction (LLE) with a three-phase solvent system (n-hexane–ethyl acetate–acetonitrile–water = 7:3:5:5, v/v/v/v) followed by separation using high-speed counter-current chromatography (HSCCC). The results showed that a 480.2-mg fraction of baf A1-enriched extract in the middle phase of the three-phase solvent system was prepared from 4.9 g of crude extract after two consecutive one-step operations. Over 99% of soybean oil, the main hydrophobic waste in the crude extract, and the majority of hydrophilic impurities were distributed in the upper and lower phase, respectively. HSCCC was used with a two-phase solvent system composed of n-hexane–acetonitrile–water (15:8:12, v/v/v) to isolate and purify baf A1 from the middle phase fraction, which yielded 77.4 mg of baf A1 with > 95% purity within 90 min. The overall recovery of baf A1 in the process was determined to be 95.7%. The use of a three-phase solvent system represents a novel strategy for the simultaneous removal of hydrophobic oil and hydrophilic impurities from a microbial fermentation extract. View Full-Text
Keywords: three-phase solvent system; oil removal; liquid–liquid extraction; bafilomycin; HSCCC three-phase solvent system; oil removal; liquid–liquid extraction; bafilomycin; HSCCC
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Yuan, Y.; He, X.; Wang, T.; Zhang, X.; Li, Z.; Xu, X.; Zhang, W.; Yan, X.; Li, S.; He, S. Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography. Mar. Drugs 2020, 18, 332.

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