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Open AccessArticle

A Phenotarget Approach for Identifying an Alkaloid Interacting with the Tuberculosis Protein Rv1466

1
Griffith Institute for Drug Discovery, Griffith University, Brisbane, Queensland 4111, Australia
2
Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, 530200, China
3
Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99354, USA
4
School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA
5
Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA 98109-5219, USA
6
Departments of Pediatrics, Global Health, and Biomedical Informatics & Medical Education, University of Washington, Seattle, WA 98195, USA
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(3), 149; https://doi.org/10.3390/md18030149
Received: 29 December 2019 / Revised: 11 February 2020 / Accepted: 20 February 2020 / Published: 5 March 2020
(This article belongs to the Special Issue Selected Papers from XVI MaNaPro and XI ECMNP)
In recent years, there has been a revival of interest in phenotypic-based drug discovery (PDD) due to target-based drug discovery (TDD) falling below expectations. Both PDD and TDD have their unique advantages and should be used as complementary methods in drug discovery. The PhenoTarget approach combines the strengths of the PDD and TDD approaches. Phenotypic screening is conducted initially to detect cellular active components and the hits are then screened against a panel of putative targets. This PhenoTarget protocol can be equally applied to pure compound libraries as well as natural product fractions. Here we described the use of the PhenoTarget approach to identify an anti-tuberculosis lead compound. Fractions from Polycarpa aurata were identified with activity against Mycobacterium tuberculosis H37Rv. Native magnetic resonance mass spectrometry (MRMS) against a panel of 37 proteins from Mycobacterium proteomes showed that a fraction from a 95% ethanol re-extraction specifically formed a protein-ligand complex with Rv1466, a putative uncharacterized Mycobacterium tuberculosis protein. The natural product responsible was isolated and characterized to be polycarpine. The molecular weight of the ligand bound to Rv1466, 233 Da, was half the molecular weight of polycarpine less one proton, indicating that polycarpine formed a covalent bond with Rv1466. View Full-Text
Keywords: PhenoTarget approach; MRMS; protein-ligand complex; polycarpine PhenoTarget approach; MRMS; protein-ligand complex; polycarpine
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MDPI and ACS Style

Xie, Y.; Feng, Y.; Di Capua, A.; Mak, T.; Buchko, G.W.; Myler, P.J.; Liu, M.; Quinn, R.J. A Phenotarget Approach for Identifying an Alkaloid Interacting with the Tuberculosis Protein Rv1466. Mar. Drugs 2020, 18, 149.

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