Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus
1
Division of Molecular Biology, Ruđer Bošković Institute, 10000 Zagreb, Croatia
2
Ministry of Health of the Republic of Croatia, 10000 Zagreb, Croatia
3
Division of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, Croatia
4
Forensic Science Centre “Ivan Vučetić”, 10000 Zagreb, Croatia
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Mar. Drugs 2020, 18(1), 13; https://doi.org/10.3390/md18010013
Received: 28 November 2019
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Revised: 18 December 2019
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Accepted: 19 December 2019
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Published: 21 December 2019
(This article belongs to the Special Issue Genetics of Marine Organisms Associated with Human Health)
The Nme gene/protein family of nucleoside diphosphate kinases (NDPK) was originally named after its member Nm23-H1/Nme1, the first identified metastasis suppressor. Human Nme proteins are divided in two groups. They all possess nucleoside diphosphate kinase domain (NDK). Group I (Nme1-Nme4) display a single type NDK domain, whereas Group II (Nme5-Nme9) display a single or several different NDK domains, associated or not associated with extra-domains. Data strongly suggest that, unlike Group I, none of the members of Group II display measurable NDPK activity, although some of them autophosphorylate. The multimeric form is required for the NDPK activity. Group I proteins are known to multimerize, while there are no data on the multimerization of Group II proteins. The Group II ancestral type protein was shown to be conserved in several species from three eukaryotic supergroups. Here, we analysed the Nme protein from an early branching eukaryotic lineage, the red alga Chondrus crispus. We show that the ancestral type protein, unlike its human homologue, was fully functional multimeric NDPK with high affinity to various types of DNA and dispersed localization throughout the eukaryotic cell. Its overexpression inhibits both cell proliferation and the anchorage-independent growth of cells in soft agar but fails to deregulate cell apoptosis. We conclude that the ancestral gene has changed during eukaryotic evolution, possibly in correlation with the protein function.
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Keywords:
Nme gene/protein family; Nme5; red alga; Chondrus crispus; eukaryotic evolution; Nme evolution
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MDPI and ACS Style
Perina, D.; Korolija, M.; Mikoč, A.; Halasz, M.; Herak Bosnar, M.; Ćetković, H. Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus. Mar. Drugs 2020, 18, 13. https://doi.org/10.3390/md18010013
AMA Style
Perina D, Korolija M, Mikoč A, Halasz M, Herak Bosnar M, Ćetković H. Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus. Marine Drugs. 2020; 18(1):13. https://doi.org/10.3390/md18010013
Chicago/Turabian StylePerina, Dragutin, Marina Korolija, Andreja Mikoč, Mirna Halasz, Maja Herak Bosnar, and Helena Ćetković. 2020. "Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus" Marine Drugs 18, no. 1: 13. https://doi.org/10.3390/md18010013
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