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Open AccessArticle

Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin

1
School of Medicine and Pharmacy and College of Food Science and Technology, Ocean University of China, Qingdao 266003, China
2
School of Food Science and Technology, Qingdao Agricultural University, Qingdao 266109, China
3
School of Sports Media and Information Technology, Shandong Sport University, Rizhao 276826, China
4
Technical Center of Entry-Exit Inspection and Quarantine, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China
5
Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
6
Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(8), 450; https://doi.org/10.3390/md17080450
Received: 11 July 2019 / Revised: 27 July 2019 / Accepted: 29 July 2019 / Published: 31 July 2019
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Abstract

The effect of collagen peptides (CPs) in intestinal mucosal protection has been approved in both cell and animal models. However, its structure–activity relationship and efficient peptide sequences are unclear, which hinders the in-depth study of its action mechanism and relative nutraceuticals and pharmaceuticals development. In this work, size exclusion chromatography, cation-exchange chromatography, and RP-HPLC were used to separate Alaska pollock skin-derived collagen hydrolysates based on their molecular weight, charge property, and hydrophobicity. The intestinal epithelial barrier function (IEBF) protective effect of separated peptide fractions were evaluated by tumor necrosis factor (TNF)-α-induced Caco-2 cell model. Results indicated that lower molecular weight (500–1000 Da) and higher hydrophilicity of CPs were related to better IEBF protective effect. Two high-efficiency IEBF protective peptide sequences, GPSGPQGSR and GPSGLLGPK with the corresponding molecular weights of 841.41 Da and 824.38 Da, were subsequently identified by UPLC-QToF-MS/MS. Their IEBF protective ability are comparable or even better than the currently used intestinal health supplements glutamine and arginine. The present findings suggested that the hydrophilic CPs, with molecular weight between 500 Da to 1000 Da, should be preferred in IEBF protective peptides preparation. GPSGPQGSR and GPSGLLGPK might have the potential of being IEBF protective ingredients used in intestinal health supplements and drugs. View Full-Text
Keywords: collagen peptide; intestinal epithelium; barrier function; intestinal health; structure–activity relationship collagen peptide; intestinal epithelium; barrier function; intestinal health; structure–activity relationship
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Song, W.; Chen, Q.; Wang, Y.; Han, Y.; Zhang, H.; Li, B.; Yu, G. Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin. Mar. Drugs 2019, 17, 450.

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