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Open AccessArticle

The Marine Catenovulum agarivorans MNH15 and Dextranase: Removing Dental Plaque

by Xiaohua Lai 1,2,†, Xin Liu 1,2,†, Xueqin Liu 1,2, Tian Deng 1,2, Yanli Feng 1,2, Xiaopeng Tian 1,2, Mingsheng Lyu 1,2,3,* and Shujun Wang 1,2,3,*
1
Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China
2
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China
3
Collaborative Innovation Center of Modern Biological Manufacturing, Anhui University, Hefei 230039, China
*
Authors to whom correspondence should be addressed.
These authors are contributed equally to this work.
Mar. Drugs 2019, 17(10), 592; https://doi.org/10.3390/md17100592
Received: 29 August 2019 / Revised: 15 October 2019 / Accepted: 16 October 2019 / Published: 18 October 2019
Dextranase, a hydrolase that specifically hydrolyzes α-1,6-glucosidic bonds, has been used in the pharmaceutical, food, and biotechnology industries. In this study, the strain of Catenovulum agarivorans MNH15 was screened from marine samples. When the temperature, initial pH, NaCl concentration, and inducer concentration were 30 °C, 8.0, 5 g/L, and 8 g/L, respectively, it yielded more dextranase. The molecular weight of the dextranase was approximately 110 kDa. The maximum enzyme activity was achieved at 40 °C and a pH of 8.0. The enzyme was stable at 30 °C and a pH of 5–9. The metal ion Sr2+ enhanced its activity, whereas NH4+, Co2+, Cu2+, and Li+ had the opposite effect. The dextranase effectively inhibited the formation of biofilm by Streptococcus mutans. Moreover, sodium fluoride, xylitol, and sodium benzoate, all used in dental care products, had no significant effect on dextranase activity. In addition, high-performance liquid chromatography (HPLC) showed that dextran was mainly hydrolyzed to glucose, maltose, and maltoheptaose. The results indicated that dextranase has high application potential in dental products such as toothpaste and mouthwash. View Full-Text
Keywords: Catenovulum agarivorans; dextranase; dental plaque; oligo-saccharides Catenovulum agarivorans; dextranase; dental plaque; oligo-saccharides
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Lai, X.; Liu, X.; Liu, X.; Deng, T.; Feng, Y.; Tian, X.; Lyu, M.; Wang, S. The Marine Catenovulum agarivorans MNH15 and Dextranase: Removing Dental Plaque. Mar. Drugs 2019, 17, 592.

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