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Open AccessArticle

Anti-Inflammatory Activity of a Peptide from Skipjack (Katsuwonus pelamis)

1
Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China
2
College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China
3
Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan Center for Disease Control and Prevention, Zhoushan 316021, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(10), 582; https://doi.org/10.3390/md17100582
Received: 13 September 2019 / Revised: 10 October 2019 / Accepted: 10 October 2019 / Published: 13 October 2019
In this paper, the effect of skipjack (Katsuwonus pelamis) enzymatic peptide (SEP), which was prepared and purified from a byproduct of skipjack, on inflammation, ulcerative colitis and the regulation of intestinal flora was studied in a mouse ulcerative colitis model and a transgenic zebrafish inflammation model. The aggregation of transgenic granulocyte neutrophils in zebrafish from a normal environment and from a sterile environment was calculated, and the anti-inflammatory activity of SEP was evaluated. To evaluate the anti-ulcerative colitis activity of SEP, DSS-induced colitis mice were given SEP, salicylazosulfapyridine (SASP), or SASP + SEP. Then, the concentrations of IL-6, IL-10 and TNF-α in the serum were detected, the HE-stained colon tissue was examined by microscopy the species composition and abundance distribution of the intestinal flora was analyzed. The results showed that 500 μg/mL SEP treatment significantly alleviated neutrophil granulocyte aggregation in the zebrafish inflammation model; Diarrhea, hematochezia and body weight loss were alleviated to a certain extent in mice gavaged with SEP and SASP, and the combination of SASP with SEP was the most effective in mice. The damage to villi in the intestine was completely repaired, and the levels of IL-6, IL-10 and TNF-α, which are associated with inflammation, were all reduced. In addition, the proportion of intestinal probiotics or harmless bacteria increased, while that of pathogenic bacteria decreased, and the effect of the combined treatment was the most pronounced. These results show that SEP could relieve inflammation, cure ulcerative colitis, regulate intestinal flora and enhance the therapeutic effect of the clinical drug SASP. This study provides a theoretical basis for the development of SEP as an anti-inflammatory adjuvant therapy and intestinal flora regulator. View Full-Text
Keywords: skipjack enzymatic peptide; transgenic zebrafish; neutrophil granulocytes; ulcerative colitis; intestinal flora skipjack enzymatic peptide; transgenic zebrafish; neutrophil granulocytes; ulcerative colitis; intestinal flora
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Wang, Z.-G.; Ying, X.-G.; Gao, P.; Wang, C.-L.; Wang, Y.-F.; Yu, X.-W.; Chen, J.; Wang, B.; Luo, H.-Y. Anti-Inflammatory Activity of a Peptide from Skipjack (Katsuwonus pelamis). Mar. Drugs 2019, 17, 582.

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