Fucoidan–Fucoxanthin Ameliorated Cardiac Function via IRS1/GRB2/ SOS1, GSK3β/CREB Pathways and Metabolic Pathways in Senescent Mice
Graduate Institute of Biotechnology, Chinese Culture University, Taipei 11114, Taiwan
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(1), 69; https://doi.org/10.3390/md17010069
Received: 3 November 2018
/
Revised: 12 January 2019
/
Accepted: 18 January 2019
/
Published: 21 January 2019
The effects of low molecular weight fucoidan (LMWF) in combination with high-stability fucoxanthin (HSFUCO) on cardiac function and the metabolic pathways of aging mice (Mus musculus) were investigated. We demonstrated that LMWF and HSFUCO could improve cardiac function in aging mice. Aging mice were treated with LMWF and HSFUCO, either on their own or in combination, on 28 consecutive days. Electrocardiography and whole-cell patch-clamp were used to measure QT interval and action potential duration (APD) of the subjects. Cardiac tissue morphology, reactive oxygen species, and Western blot were also applied. Ultra-high-performance liquid chromatography–quadrupole time-of-flight (UPLC-QTOF) mass spectrometry was used for investigating metabolic alterations. The use of LMWF and HSFUCO resulted in improvements in both ventricular rhythms (QT and APD). Treatment with fucoidan and fucoxanthin reduced the expression levels of SOS1 and GRB2 while increasing GSK3β, CREB and IRS1 proteins expression in the aging process. Three main metabolic pathways, namely the TCA cycle, glycolysis, and steroid hormone biosynthesis, were highly enriched in the pathway enrichment analysis. When taken together, the LMWF and HSFUCO treatment improved both the ventricular rhythm and the muscular function of aging subjects by interfering with the metabolism and gene function.
View Full-Text
Keywords:
fucoidan; fucoxanthin; aging mice; metabolomics; long QT
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Supplementary File 1:
PDF-Document (PDF, 204 KiB)
MDPI and ACS Style
Chang, P.-M.; Li, K.-L.; Lin, Y.-C. Fucoidan–Fucoxanthin Ameliorated Cardiac Function via IRS1/GRB2/ SOS1, GSK3β/CREB Pathways and Metabolic Pathways in Senescent Mice. Mar. Drugs 2019, 17, 69. https://doi.org/10.3390/md17010069
AMA Style
Chang P-M, Li K-L, Lin Y-C. Fucoidan–Fucoxanthin Ameliorated Cardiac Function via IRS1/GRB2/ SOS1, GSK3β/CREB Pathways and Metabolic Pathways in Senescent Mice. Marine Drugs. 2019; 17(1):69. https://doi.org/10.3390/md17010069
Chicago/Turabian StyleChang, Po-Ming, Kuan-Lun Li, and Yen-Chang Lin. 2019. "Fucoidan–Fucoxanthin Ameliorated Cardiac Function via IRS1/GRB2/ SOS1, GSK3β/CREB Pathways and Metabolic Pathways in Senescent Mice" Marine Drugs 17, no. 1: 69. https://doi.org/10.3390/md17010069
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
