Next Article in Journal
Blueprints for the Next Generation of Bioinspired and Biomimetic Mineralised Composites for Bone Regeneration
Previous Article in Journal
Anti-Photoaging Effects of Low Molecular-Weight Fucoidan on Ultraviolet B-Irradiated Mice
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Mar. Drugs 2018, 16(8), 287; https://doi.org/10.3390/md16080287

Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

1
Department of General Chemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No.1, Bucharest 011061, Romania
2
Department of Biomaterials, National Institute of Research and Development for Biological Sciences, Bioproducts, Splaiul Independentei, No.296, Bucharest 060031, Romania
*
Author to whom correspondence should be addressed.
Received: 30 June 2018 / Revised: 28 July 2018 / Accepted: 17 August 2018 / Published: 18 August 2018
(This article belongs to the Special Issue Marine Metabolites and Metal Ion Chelation)
Full-Text   |   PDF [2174 KB, uploaded 18 August 2018]   |  

Abstract

In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+. The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions. View Full-Text
Keywords: chitosan; alkaline phosphatase; metal ion; hemolytic index; cell viability chitosan; alkaline phosphatase; metal ion; hemolytic index; cell viability
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Tihan, G.T.; Zgarian, R.G.; Berteanu, E.; Ionita, D.; Totea, G.; Iordachel, C.; Tatia, R.; Prodana, M.; Demetrescu, I. Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications. Mar. Drugs 2018, 16, 287.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top