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Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

Department of General Chemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No.1, Bucharest 011061, Romania
Department of Biomaterials, National Institute of Research and Development for Biological Sciences, Bioproducts, Splaiul Independentei, No.296, Bucharest 060031, Romania
Author to whom correspondence should be addressed.
Mar. Drugs 2018, 16(8), 287;
Received: 30 June 2018 / Revised: 28 July 2018 / Accepted: 17 August 2018 / Published: 18 August 2018
(This article belongs to the Special Issue Marine Metabolites and Metal Ion Chelation)
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In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+. The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions. View Full-Text
Keywords: chitosan; alkaline phosphatase; metal ion; hemolytic index; cell viability chitosan; alkaline phosphatase; metal ion; hemolytic index; cell viability

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Tihan, G.T.; Zgarian, R.G.; Berteanu, E.; Ionita, D.; Totea, G.; Iordachel, C.; Tatia, R.; Prodana, M.; Demetrescu, I. Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications. Mar. Drugs 2018, 16, 287.

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