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Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench

by Yanan Li 1,2, Xue Kong 1,2, Jiawei Chen 1,2, Helu Liu 1 and Haibin Zhang 1,*
1
Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China
2
College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100039, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2018, 16(5), 169; https://doi.org/10.3390/md16050169
Received: 3 April 2018 / Revised: 11 May 2018 / Accepted: 15 May 2018 / Published: 18 May 2018
Superoxide dismutases (SODs) are among the most important antioxidant enzymes and show great potential in preventing adverse effects during therapeutic trials. In the present study, cloning, expression, and characterization of a novel Cu,Zn superoxide dismutase (Ps-Cu,Zn-SOD) from a hadal sea cucumber (Paelopatides sp.) were reported. Phylogenetic analysis showed that Ps-Cu,Zn-SOD belonged to a class of intracellular SOD. Its Km and Vmax were 0.0258 ± 0.0048 mM and 925.1816 ± 28.0430 units/mg, respectively. The low Km value of this enzyme represents a high substrate affinity and can adapt to the low metabolic rate of deep sea organisms. The enzyme functioned from 0 °C to 80 °C with an optimal temperature of 40 °C. Moreover, the enzyme activity was maintained up to 87.12% at 5 °C. The enzyme was active at pH 4 to 12 with an optimal pH of 8.5. Furthermore, Ps-Cu,Zn-SOD tolerated high concentration of urea and GuHCl, resisted hydrolysis by proteases, and maintained stability at high pressure. All these features demonstrated that the deep sea Ps-Cu,Zn-SOD is a potential candidate for application to the biopharmaceutical field. View Full-Text
Keywords: superoxide dismutase (SOD); Paelopatides; expression; pressure; Mariana Trench; deep sea; sea cucumber superoxide dismutase (SOD); Paelopatides; expression; pressure; Mariana Trench; deep sea; sea cucumber
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Li, Y.; Kong, X.; Chen, J.; Liu, H.; Zhang, H. Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench. Mar. Drugs 2018, 16, 169.

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