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Mar. Drugs 2017, 15(5), 140;

Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells

Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
Author to whom correspondence should be addressed.
Academic Editors: Paul Long, Orazio Taglialatela-Scafati and Alejandro M. Mayer
Received: 16 March 2017 / Revised: 24 April 2017 / Accepted: 4 May 2017 / Published: 18 May 2017
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In this study, the extraction conditions of the crude polysaccharide from Cereus sinensis were optimized by response surface methodology. The optimum extraction conditions were: a ratio of raw material to water volume of 1:80 (g/mL); an extraction temperature of 72 °C; and an extraction time of 3 h. Then, a purified polysaccharide named Cereus sinensis polysaccharide-1 (CSP-1) was obtained from the crude polysaccharide by the Diethylaminoethyl cellulose-52 (DEAE-52) cellulose chromatography column and Sephadex G-100 column. The molecular weight and monosaccharide composition of CSP-1 was determined through Gel Permeation Chromatography (GPC) and Gas Chromatography–Mass Spectrometer (GS–MS), respectively. The results showed that CSP-1 with an average molecular weight of 56,335 Da was composed of l-(−)-Fucose, d-(+)-Mannose, d-Glucose and mainly possessed 1→2, 1→2, 6, 1→4, and 1→4, 6 of glycosyl linkages. The immunomodulatory activities of CSP-1 were also evaluated using lipopolysaccharide (LPS)-induced human monocytic (THP-1) cells. The results demonstrated that CSP-1 dose-dependently protected against LPS-induced toxicity, and CSP-1 significantly inhibited the Toll-like receptor 4 (TLR-4) mRNA, myeloid differentiation factor 88 (MyD88) mRNA and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA expression of the LPS-induced THP-1 cells, as well as suppressing reactive oxygen species (ROS) generation. View Full-Text
Keywords: Cereus sinensis; polysaccharide; THP-1 cells; immunomodulatory property Cereus sinensis; polysaccharide; THP-1 cells; immunomodulatory property

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Wu, J.; Zhou, X.; Zhang, M.; Yao, Y.; Han, J.; Liu, K. Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells. Mar. Drugs 2017, 15, 140.

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