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Mar. Drugs 2017, 15(11), 347;

Immunoenhancement Effects of Glycosaminoglycan from Apostichopus japonicus: In Vitro and In Cyclophosphamide-Induced Immunosuppressed Mice Studies

School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, Shandong, China
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, Shandong, China
Key Laboratory of Marine Drugs, Ministry of Education of China, Qingdao 266003, Shandong, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 6 October 2017 / Revised: 31 October 2017 / Accepted: 3 November 2017 / Published: 7 November 2017
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In this study, the immunomodulatory activities of Apostichopus japonicus glycosaminoglycan (AHG) on the nature killer (NK) cells, cytotoxic T lymphocytes (CTLs) and cyclophosphamide (CY)-treated mice were investigated. After stimulation with multiple concentrations of AHG (0–100 μg/mL), NK cells and CTLs displayed outperformance against YAC-1 and B16 cells, respectively. Furthermore, the mitogen-induced splenic lymphocyte proliferation in CY-induced immunosuppressed mice was significantly promoted by AHG. In addition, the administration of AHG dramatically increased the splenocytes Ca2+ concentration and the delayed-type hypersensitivity (DTH) reaction in a dose-dependent manner. Besides, AHG could strongly increase the total antioxidant capacity (T-AOC), the activities of superoxidase dismutase (SOD), catalase (CAT) as well as glutathione peroxidase (GSH-PX), and could decrease the malondialdehyde (MDA) level in the heart, kidney and liver. These findings indicated that AHG played an important role in the immune enhancement and protection against CY-induced immunosuppression and oxidative damage. Our findings provide experimental evidence for further research and possible immunostimulatory applications of AHG in clinical practice. View Full-Text
Keywords: sea cucumber; glycosaminoglycan; cyclophosphamide; immunomodulation sea cucumber; glycosaminoglycan; cyclophosphamide; immunomodulation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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    Doi: 10.1016/j.ijbiomac.2014.10.010
    Description: Stucture of AHG

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Wang, H.; Yang, S.; Wang, Y.; Jiang, T.; Li, S.; Lv, Z. Immunoenhancement Effects of Glycosaminoglycan from Apostichopus japonicus: In Vitro and In Cyclophosphamide-Induced Immunosuppressed Mice Studies. Mar. Drugs 2017, 15, 347.

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