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Dioxinodehydroeckol Enhances the Differentiation of Osteoblasts by Regulating the Expression of Phospho-Smad1/5/8

Department of Organic Material Science and Engineering, Pusan National University, Busan 46241, Korea
Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Busan 46958, Korea
Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958, Korea
Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Korea
Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University, Busan 49112, Korea
Department of Convergence Study on the Ocean Science and Technology, Ocean Science and Technology School, Korea Maritime and Ocean University, Busan 49112, Korea
Author to whom correspondence should be addressed.
Academic Editor: Peer B. Jacobson
Mar. Drugs 2016, 14(9), 168;
Received: 9 June 2016 / Revised: 1 September 2016 / Accepted: 7 September 2016 / Published: 14 September 2016
(This article belongs to the Special Issue Marine Natural Products that Target Metabolic Disorders)
PDF [2202 KB, uploaded 14 September 2016]


Lack of bone formation-related health problems are a major problem for the aging population in the modern world. As a part of the ongoing trend of developing natural substances that attenuate osteoporotic bone loss conditions, dioxinodehydroeckol (DHE) from edible brown alga Ecklonia cava was tested for its effects on osteoblastogenic differentiation in MC3T3-E1 pre-osteoblasts. DHE was observed to successfully enhance osteoblast differentiation, as indicated by elevated cell proliferation, alkaline phosphatase activity, intracellular cell mineralization, along with raised levels of osteoblastogenesis indicators at the concentration of 20 μM. Results suggested a possible intervening of DHE on the bone morphogenetic protein (BMP) signaling pathway, according to elevated protein levels of BMP-2, collagen-I, and Smads. In addition, the presence of DHE was also able to raise the phosphorylated extracellular signal–regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) levels which are also activated by the BMP signaling pathway. In conclusion, DHE is suggested to be a potential bioactive compound against bone loss that could enhance osteoblastogenesis with a suggested BMP pathway interaction. View Full-Text
Keywords: BMP-2; dioxinodehydroeckol; Ecklonia cava; osteoblastogenesis; Smad BMP-2; dioxinodehydroeckol; Ecklonia cava; osteoblastogenesis; Smad

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Ahn, B.-N.; Karadeniz, F.; Kong, C.-S.; Nam, K.-H.; Jang, M.-S.; Seo, Y.; Kim, H.S. Dioxinodehydroeckol Enhances the Differentiation of Osteoblasts by Regulating the Expression of Phospho-Smad1/5/8. Mar. Drugs 2016, 14, 168.

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