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Open AccessArticle

Resonance Rayleigh Scattering Spectra of an Ion-Association Complex of Naphthol Green B–Chitosan System and Its Application in the Highly Sensitive Determination of Chitosan

by Weiai Zhang 1, Caijuan Ma 1, Zhengquan Su 1,2,* and Yan Bai 1,*
1
School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China
2
Key Research Center of Liver Regulation for Hyperlipidemia SATCM/Class III Laboratory of Metabolism SATCM, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Hitoshi Sashiwa and David Harding
Mar. Drugs 2016, 14(4), 71; https://doi.org/10.3390/md14040071
Received: 29 October 2015 / Revised: 28 March 2016 / Accepted: 29 March 2016 / Published: 18 April 2016
(This article belongs to the Special Issue Advances in Marine Chitin and Chitosan II, 2017)
This work describes a highly-sensitive and accurate approach for the determination of chitosan (CTS) using Naphthol Green B (NGB) as a probe in the Resonance Rayleigh scattering (RRS) method. The interaction between CTS and NGB leads to notable enhancement of RRS, and the enhancement is proportional to the concentration of CTS over a certain range. Under optimum conditions, the calibration curve of ΔI against CTS concentration was ΔI = 1860.5c + 86.125 (c, µg/mL), R2 = 0.9999, and the linear range and detection limit (DL) were 0.01–5.5 µg/mL and 8.87 ng/mL. Moreover, the effect of the molecular weight of CTS on the accurate quantification of CTS was studied. The experimental data were analyzed through linear regression analysis using SPSS20.0, and the molecular weight was found to have no statistical significance. This method has been applied to assay two CTS samples and obtained good recovery and reproducibility. View Full-Text
Keywords: chitosan; Naphthol Green B; Resonance Rayleigh scattering spectra chitosan; Naphthol Green B; Resonance Rayleigh scattering spectra
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MDPI and ACS Style

Zhang, W.; Ma, C.; Su, Z.; Bai, Y. Resonance Rayleigh Scattering Spectra of an Ion-Association Complex of Naphthol Green B–Chitosan System and Its Application in the Highly Sensitive Determination of Chitosan. Mar. Drugs 2016, 14, 71.

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