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Mar. Drugs 2016, 14(11), 195;

Macrolactins from Marine-Derived Bacillus subtilis B5 Bacteria as Inhibitors of Inducible Nitric Oxide and Cytokines Expression

Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, China
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, China
These authors contribute equally to this paper.
Present address: West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Authors to whom correspondence should be addressed.
Academic Editor: Keith B. Glaser
Received: 25 June 2016 / Revised: 26 September 2016 / Accepted: 11 October 2016 / Published: 26 October 2016
(This article belongs to the Special Issue Deep-Sea Natural Products)
Full-Text   |   PDF [1048 KB, uploaded 26 October 2016]   |  


In order to find new natural products with anti-inflammatory activity, chemical investigation of a 3000-meter deep-sea sediment derived bacteria Bacillus subtilis B5 was carried out. A new macrolactin derivative was isolated and identified as 7,13-epoxyl-macrolactin A (1). Owing to the existence of the epoxy ring, 1 exhibited a significant inhibitory effect on the expression of inducible nitric oxide and cytokines, compared with previously isolated known macrolactins (25). Real-time Polymerase Chain Reaction (PCR) analysis showed that the new compound significantly inhibited the mRNA expressions of inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Reverse transcription-PCR analysis demonstrated that the new compound reduced the mRNA expression level of IL-1β in a concentration-dependent manner. View Full-Text
Keywords: Bacillus subtilis B5; 7,13-epoxyl-macrolactin A; macrolactins; anti-inflammatory Bacillus subtilis B5; 7,13-epoxyl-macrolactin A; macrolactins; anti-inflammatory

Figure 1

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Yan, X.; Zhou, Y.-X.; Tang, X.-X.; Liu, X.-X.; Yi, Z.-W.; Fang, M.-J.; Wu, Z.; Jiang, F.-Q.; Qiu, Y.-K. Macrolactins from Marine-Derived Bacillus subtilis B5 Bacteria as Inhibitors of Inducible Nitric Oxide and Cytokines Expression. Mar. Drugs 2016, 14, 195.

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