Sinulolides A–H, New Cyclopentenone and Butenolide Derivatives from Soft Coral Sinularia sp.

Abstract

Eight new compounds, sinulolides A–H (1–8), along with two known compounds, α-methoxy-2,3-dimethyl-butenolide (9) and sinularone D (10), were isolated from the soft coral Sinularia sp. The structures of these compounds were elucidated on the basis of extensive spectroscopic analysis. The absolute configurations were determined on the basis of electronic circular dichroism (ECD) data analysis. Compounds 5 and 10 exhibited moderate effects for the inhibition of NF-κB activation.

1. Introduction

Genus Sinularia is a soft coral belonging to the phylum, Cnidaria, class Alcyonaria and family Alcyoniidae. It constitutes a dominant portion of the biomass in the tropical reef environment [1]. Many bioactive metabolites, including sesquiterpenes [2,3,4], diterpenes [5,6,7,8,9] and polyhydroxylated steroids [10,11,12], have been studied, and the isolated components display a range of biological activities, such as antimicrobial, anti-inflammatory, glucose transport in rat adipocytes and cytotoxic activities [13,14,15,16,17]. During the course of our investigation on the bioactive chemical constituents from the soft coral, eight new compounds, sinulolides A–H (1–8), along with two known compounds, α-methoxy-2,3-dimethyl-butenolide (9) and sinularone D (10) (Figure 1), were isolated from Sinularia sp., collected off the Dongluo Island, Sanya, in July, 2009, at a depth of 10 m. The bioactivities of these compounds were determined through bioactivity tests using high-throughput screening (HTS). We describe herein the isolation, structure elucidation and bioactivities of these compounds.

Figure 1. Structures of metabolites 1–10.

Figure 1. Structures of metabolites 1–10.

2. Results and Discussion

Compound 1 was isolated as a colorless oil. Its molecular formula was assigned as C₂₀H₃₀O₆ based on the HRESIMS at m/z 367.2117 [M + H]⁺, accounting for six degrees of unsaturation. The ¹H NMR spectrum indicated the presence of one methoxyl singlet (δ_H 3.66, s, H-16), four methyl singlets (δ_H 2.01, H-1, 1.83, H-19, 1.82, H-20, 1.04, H-18), one methyl doublet (δ_H 1.15, t, J = 7.0 Hz, H-17) and a low-field exchangeable hydroxyl proton (δ_H 4.72, s, H-3), in addition to 11 aliphatic protons (Table 1). The ¹³C-NMR and HMQC spectra of 1 showed the presence of six methyls, five methylenes, one methine and eight quaternary carbons, including two olefinic carbons (δ_C 163.1, 139.5), three oxygen-bearing quaternary carbons (δ_C 89.1, 92.3, 86.7), two ketones (δ_C 207.0, 204.7) and one carbonyl (δ_C 177.4). The above functionalities account for four of the six degrees of unsaturation in the molecule, revealing a bicyclic structure for 1. The ¹H and ¹³C NMR spectra of 1 were similar to those of α-tocospiro A [18,19], with the difference of the side-chain, which was confirmed by the HMBC experiment. The HMBC interactions from H₃-16 to C-15, H-13 to C-12, C-14, C-15 and C-17, H-14 to C-12, C-13, C-15 and C-17 and H₃-17 to C-13, C-14 and C-15 led to the connectivity of the subunits to form a linear chain (Figure 2). Subsequently, the linear side chain was determined to be linked to the nucleus at C-10 on the basis of the HMBC interactions from H₃-18 to C-9, C-10 and C-11. For the spiro moiety, the relative stereochemistry was shown to be the same as that of α-tocospiro A on the basis of similar specific rotation and the NOESY spectrum. Circular dichroism (CD) data also support the absolute configuration of 1 to be identical to that of α-tocospiro C [20]. The absolute configuration was further determined by electronic circular dichroism (ECD) (Figure 3). The calculated ECD showed diagnostic cotton effects around 226 (positive), 246 (negative) and 287 (positive) nm, consistent with the experimental ECD. Thus, the absolute configuration was established as 3S, 7R and 10S, whereas the configurations at C-14 remained to be determined.

Table 1. ¹H and ¹³CNMR spectroscopic data for compounds 1 and 2 (500/125 MHz, in CDCl₃, δ in ppm, J in Hz).

Position	1		2
	13C	1H	13C	1H
1	25.0	2.01 s	24.8	2.01 s
2	207.0		207.0
3	89.1	4.72 s	89.4	4.68 s
4	163.1		163.2
5	139.5		139.5
6	204.7		205.0
7	92.3		92.7
8	36.5	1.87 m	36.8	1.88 m
		1.76 m		1.76 m
9	33.0	2.41 m	33.3	2.38 m
		1.76 m		1.76 m
10	86.7		86.9
11	41.0	1.62 m	41.4	1.62 m
12	22.5	1.41 m	22.5	1.41 m
		1.36 m		1.36 m
13	34.4	1.67 m	34.2	1.64 m
		1.41 m		1.40 m
14	39.5	2.48 m	39.3	2.44 m
15	177.4		177.2
16	51.5	3.66 s	51.5	3.67 s
17	17.2	1.15 d (7.0)	17.0	1.14 d (7.0)
18	25.7	1.04 s	25.4	1.29 s
19	12.0	1.83 s	11.8	1.83 s
20	8.9	1.82 s	8.7	1.81 s

Table 1. ¹H and ¹³CNMR spectroscopic data for compounds 1 and 2 (500/125 MHz, in CDCl₃, δ in ppm, J in Hz).

Position	1		2
	13C	1H	13C	1H
1	25.0	2.01 s	24.8	2.01 s
2	207.0		207.0
3	89.1	4.72 s	89.4	4.68 s
4	163.1		163.2
5	139.5		139.5
6	204.7		205.0
7	92.3		92.7
8	36.5	1.87 m	36.8	1.88 m
		1.76 m		1.76 m
9	33.0	2.41 m	33.3	2.38 m
		1.76 m		1.76 m
10	86.7		86.9
11	41.0	1.62 m	41.4	1.62 m
12	22.5	1.41 m	22.5	1.41 m
		1.36 m		1.36 m
13	34.4	1.67 m	34.2	1.64 m
		1.41 m		1.40 m
14	39.5	2.48 m	39.3	2.44 m
15	177.4		177.2
16	51.5	3.66 s	51.5	3.67 s
17	17.2	1.15 d (7.0)	17.0	1.14 d (7.0)
18	25.7	1.04 s	25.4	1.29 s
19	12.0	1.83 s	11.8	1.83 s
20	8.9	1.82 s	8.7	1.81 s

Figure 2. Key HMBC correlations of compounds 1 and 3.

Figure 2. Key HMBC correlations of compounds 1 and 3.

Figure 3. Calculated and experimental electronic circular dichroism (ECD) spectra of compound 1.

Figure 3. Calculated and experimental electronic circular dichroism (ECD) spectra of compound 1.

The NMR spectroscopic data of 1 and 2 are very similar, except for the downfield shift of CH₃-18 (1.04→1.29) (Table 1), implying 2 to be a C-10 epimer of 1, as is evident from α-tocospiro B [18,19]. However, the optical rotation and the CD spectrum of 2 are the opposite sign to the data of 1. The measured CD curve of 2 was very similar to the calculated ECD for 3R, 7S, 10S-isomer, opposite of the data for 1 (Figure 4), indicating 2 to be in agreement with 3R, 7S and 10S.

Figure 4. Calculated and experimental ECD spectra of compound 2.

Figure 4. Calculated and experimental ECD spectra of compound 2.

Compound 3 was isolated as a colorless oil. The ion peak was observed in ESI-MS at m/z 291 [M + Na]⁺, 559 [2M + Na]⁺. The ¹H NMR spectrum indicated the presence of one methoxyl singlet (δ_H 3.75, s, H-15), two methyl singlets (δ_H 2.00, H-13, 1.72, H-14) and one terminal methyl triplet (δ_H 0.83, t, J = 7.0 Hz, H-12) (Table 2). The ¹³C NMR and HMQC spectra of 3 showed the presence of four methyls, five methylenes one methine, as well as five quaternary carbons, including two olefinic carbons (δ_C 168.7, 135.5), one oxygen-bearing quaternary carbon (δ_C 80.4), one ketone (δ_C 202.8) and one carbonyl (δ_C 175.2). The ¹H and ¹³C NMR spectra of 3 were almost the same as those of sinularone B [21]. The distinction was attributed to the presence of a methyl ester to replace an ethyl ester of the known analogue, as is evident from the molecular weight of 3, to be 14 amu less than that of the latter, as well as the presence of a methoxyl group in its NMR spectra (Figure 2).

Compound 4 was isolated as a colorless oil. The ion peak was observed in ESI-MS at m/z 253 [M − H]⁻. The ¹H and ¹³C NMR spectra of 4 were almost the same as those of 3, except for the absence of a methoxyl group at C-15 (δ_C 52.4) in 4 (Table 2).

Compound 5 was isolated as a colorless oil. The ion peak was observed in ESI-MS at m/z 299 [M + H]⁺, 321 [M + Na]⁺. Comparison with 5 showed almost the same NMR spectroscopic data as 3, except for the presence of a methoxyl carbon at δ_C 54.7 and a oxygenated methine at δ_C 86.9, while a methylene signal appeared at δ_C 37.0 in 3 (Table 2). This implied that a methoxyl group is located at C-8 in 5 instead of the methylene group in 3. The assumption was confirmed by the correlations of CH₃-15 to C-8 and H-8 to C-9 and C-3 in the HMBC experiment.

Compound 6 was isolated as a colorless oil. The ion peak was observed in ESI-MS at m/z 283 [M − H]⁻. Close comparison of the ¹³C NMR spectrum of Compound 6 to that of 5 showed a general similarity, except for the absence of a methoxyl carbon at δ_C 54.7 at the C-16 position in 5 (Table 2). The measured CD curve of 3–6 was very similar to the calculated ECD for 4R, 5R-isomer and opposite of the data for sinularone B [21], indicating 3–6 to be in agreement with 4R and 5R.

Compound 7 was isolated as a colorless oil. The ion peak was observed in ESI-MS at m/z: 279 [M + Na]⁺, 535 [2M + Na]⁺. The ¹H NMR spectrum suggested the presence of two methyl singlets (δ_H 1.85, s, H-10, 11) and two methoxyl singlets (δ_H 3.66, s, H-12, 3.08, s, H-13). The ¹³C NMR spectrum indicated the presence of four methyls, four methylenes and five quaternary carbons, including two carbonyl groups, two olefinic carbons and one oxygen-bearing quaternary carbon. Both the ¹H and ¹³C NMR spectra of 7 showed a close similarity to 2,3-dimethyl butenolide [22,23], except for the absence of eight methylenes in the methoxycarbonyl side chain and the presence of an additional methoxy group. Comparison of the ¹H and ¹³C NMR spectrum of 7 and 8 revealed that there were two fewer methylenes in the side chain in 8. The negative specific rotation and the opposite Cotton effect in comparison with those of sinularone H indicated that C-4 had an R configuration [21,22].

By comparing the ¹H, ¹³C-NMR and MS data with the literature values, the known Compounds 9 and 10 were identified as ã-methoxy-2,3-dimethyl-butenolide [24] and sinularone D [21], respectively.

Table 2. ¹H and ¹³CNMR spectroscopic data for compounds 3–6 (500/125 MHz, in CDCl₃, δ in ppm, J in Hz).

Position	3		4		5		6
	13C	1H	13C	1H	13C	1H	13C	1H
1	175.2		174.3		175.5		174.4
2	29.8	3.15 dd (4.5, 15.5)	31.7	2.98 m	29.6	3.05 dd (3.0, 18.0)	28.6	3.05 dd (4.5, 8.0)
		2.39 dd (7.0, 11.5)		2.62 d (15.5)		2.69 dd (6.5, 11.5 )		2.61 dd (4.5, 14.5)
3	55.0	2.85 dd (3.5, 8.5)	46.6	2.93 m	60.9	2.98 dd (3.0, 11.5)	60.3	2.94 dd (7.0,12.5)
4	202.8		204.1		203.1		204.4
5	135.5		139.0		136.9		140.2
6	168.7		167.1		165.9		165.7
7	80.4		92.3		83.7		80.8
8	37.0	1.76 td (19.0, 5.5)	34.5	1.97 td (14.0, 4.0)	86.9	3.29 dd (4.5, 8.0)	93.0	3.61 dd (2.5, 8.5)
		1.52 td (13.0, 3.5)		1.81 m
9	25.1	0.75 m	23.3	0.88 m	31.3	1.64 m	30.6	1.68 m
		0.63 m		1.10 m		1.22 m		1.30 m
10	31.9	1.19 m	32.5	1.32 m	32.0	1.24 m	32.2	1.34 m
11	22.4	1.19 m	22.4	1.25 m	22.6	1.39 m	22.8	1.34 m
						1.07 m		1.51 m
12	13.9	0.83 t (7.0)	13.9	0.89 t (7.0)	13.9	0.83 t (7.0)	14.0	0.92 t (7.0 )
13	7.8	1.72 s	8.2	1.75 s	8.0	1.72 s	8.3	1.75 s
14	11.5	2.00 s	12.2	2.06 s	11.8	1.99 s	13.0	2.06 s
15	52.4	3.75 s			52.1	3.74 s	44.0	3.38 s
16					54.7	3.42 s

Table 2. ¹H and ¹³CNMR spectroscopic data for compounds 3–6 (500/125 MHz, in CDCl₃, δ in ppm, J in Hz).

Position	3		4		5		6
	13C	1H	13C	1H	13C	1H	13C	1H
1	175.2		174.3		175.5		174.4
2	29.8	3.15 dd (4.5, 15.5)	31.7	2.98 m	29.6	3.05 dd (3.0, 18.0)	28.6	3.05 dd (4.5, 8.0)
		2.39 dd (7.0, 11.5)		2.62 d (15.5)		2.69 dd (6.5, 11.5 )		2.61 dd (4.5, 14.5)
3	55.0	2.85 dd (3.5, 8.5)	46.6	2.93 m	60.9	2.98 dd (3.0, 11.5)	60.3	2.94 dd (7.0,12.5)
4	202.8		204.1		203.1		204.4
5	135.5		139.0		136.9		140.2
6	168.7		167.1		165.9		165.7
7	80.4		92.3		83.7		80.8
8	37.0	1.76 td (19.0, 5.5)	34.5	1.97 td (14.0, 4.0)	86.9	3.29 dd (4.5, 8.0)	93.0	3.61 dd (2.5, 8.5)
		1.52 td (13.0, 3.5)		1.81 m
9	25.1	0.75 m	23.3	0.88 m	31.3	1.64 m	30.6	1.68 m
		0.63 m		1.10 m		1.22 m		1.30 m
10	31.9	1.19 m	32.5	1.32 m	32.0	1.24 m	32.2	1.34 m
11	22.4	1.19 m	22.4	1.25 m	22.6	1.39 m	22.8	1.34 m
						1.07 m		1.51 m
12	13.9	0.83 t (7.0)	13.9	0.89 t (7.0)	13.9	0.83 t (7.0)	14.0	0.92 t (7.0 )
13	7.8	1.72 s	8.2	1.75 s	8.0	1.72 s	8.3	1.75 s
14	11.5	2.00 s	12.2	2.06 s	11.8	1.99 s	13.0	2.06 s
15	52.4	3.75 s			52.1	3.74 s	44.0	3.38 s
16					54.7	3.42 s

Using HTS, all compounds were tested toward Forkhead box O 3α (Foxo3α), 3-hydroxy-3-methylglutaryl CoA reductase gene fluorescent protein (HMGCR-GFP), nuclear factor kappa B (NF-κB) luciferase, peroxisome proliferator-activated receptor-g co-activator 1α (PGC-1α), protein-tyrosine phosphatase 1B (PTP1B), mitochondrial membrane permeabilization (MMP) and adenosine monophosphate-activated protein kinase (AMPK) activity. Compounds 3–8 and 10 were evaluated for inhibition of NF-κB activation, and the inhibitory rates are listed in Table 3. At a concentration of 10 μg/mL, sinulolide E and sinularone D exhibited moderate effects with inhibitory rates of 38.12% and 43.00%, respectively. However, all compounds were inactive against other biological targets.

Table 3. Inhibitory rates of NF-κB activation of Compounds 3–8 and 10.

Concentration	IR (%)
	3	4	5	6	7	8	10
10 μg/mL	27.85	28.75	38.12	28.24	27.08	25.28	43.00

Table 3. Inhibitory rates of NF-κB activation of Compounds 3–8 and 10.

Concentration	IR (%)
	3	4	5	6	7	8	10
10 μg/mL	27.85	28.75	38.12	28.24	27.08	25.28	43.00

3. Experimental Section

3.1. General Experimental Procedures

The NMR spectra were recorded on a Bruker AC 500NMR spectrometer (Bruker BioSpin, Fällanden, Switzerland) with tetramethylsilane (TMS) as an internal standard. ESI-MS data were measured on a Bruker amaZon SL spectrometer (Bruker, Fällanden, Switzerland). HR-ESI-MS data were measured on a Bruker micro TOF-QII mass spectrometer (Bruker, Fällanden, Switzerland). CD spectra were measured with a Chirascan circular dichroism spectrometer (Applied Photophysics Ltd., Leatherhead, UK). Optical rotation values were measured with an Anton Paar MCP500 polarimeter (Anton Paar, Graz, Austria). YMC gel (ODS-A, 12 nm, S-50 µm, YMC, Kyoto, Japan) was used for column chromatography. The SiO₂ GF₂₅₄ used for TLC was supplied by the Qingdao Marine Chemical Factory, Qingdao, China. Sephadex LH-20 gel (GE Healthcare, Uppsala, Sweden) was used. HPLC was carried out on a Hitachi L-2400 (Hitachi, Tokyo, Japan) with a YMC ODS column. Spots were detected on TLC under UV light or by heating after spraying with 5% H₂SO₄ in EtOH (v/v).

3.2. Animal Material

The soft coral Sinularia sp. was collected from Dongluo Island, Hainan province of China, in July, 2009 (7–10-m depth) and identified by Professor Hui Huang, South China Sea Institute of Oceanology, Chinese Academy of Sciences. A voucher specimen (No. 0907010) was deposited in the CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

3.3. Extraction and Isolation

The fresh soft coral (wet, 6 kg) was extracted three times with 95% EtOH (20 L). The extract was concentrated under reduced pressure and partitioned between H₂O (4 L) and CHCl₃ (4 L); the CHCl₃ layer (120 g) was further partitioned between 85% EtOH (4 L) and petroleum ether (PE; 4 L) to yield 85% EtOH (34 g) and PE (75.6 g) fractions. The 85% EtOH fraction was separated by silica gel column using CHCl₃/MeOH to yield 11 portions (Fr. s1–s11). Fr. s3 was purified by silica gel column to yield 12 portions, and Portion 10 was further purified with semi-preparative HPLC, eluting with MeOH/H₂O = 65:35 at a flow rate of 2 mL/min, to afford 1 (4.5 mg) and 2 (2.4 mg). Fr. s5 was purified by Sephadex LH-20 using CHCl₃/MeOH = 1:1 to yield 3 portions, and Portion 3 was further purified with semi-preparative HPLC, eluting with MeOH/H₂O = 57:43 at a flow rate of 2 mL/min, to afford 7 (3.0 mg), 8 (4.1 mg) and 9 (10.0 mg). Fr. s6 was further purified with semi-preparative HPLC, eluting with MeOH/H₂O = 60:40 at a flow rate of 2 mL/min, to afford 3 (2.4 mg), 4 (2.8 mg) and 10 (6.7 mg). Fr. s7 was purified by Sephadex LH-20 to yield three portions, and Portion 3 was further purified with semi-preparative HPLC, eluting with MeOH/H₂O = 60:40 at a flow rate of 2 mL/min, to afford 5 (3.5 mg) and 6 (4.2 mg).

Sinulolide A (1): colorless oil; ${\text{[α]}}_{\text{D}}^{\text{25}}$ = −172.5 (c = 0.35, MeOH); CD (MeOH; c 0.2): Δε287 −9.72, Δε246 +12.72, Δε226 −0.76; ¹H and ¹³C NMR data: see Table 1; HRESIMS m/z 367.2117 [M + H]⁺ (calcd. for C₂₀H₃₁O₆, 367.2115), 389.1942 [M + Na]⁺ (calcd. for C₂₀H₃₀O₆Na, 389.1935).

Sinulolide B (2): colorless oil; ${\text{[α]}}_{\text{D}}^{\text{25}}$ = +103.2 (c = 0.07, MeOH); CD (MeOH; c 0.2): Δε287 +3.94, Δε246 −4.66, Δε226 +0.51; ¹H and ¹³C NMR data: see Table 1; ESI-MS m/z 367 [M + H]⁺, 389 [M + Na]⁺, 755 [2M + Na]⁺.

Sinulolide C (3): colorless oil; ${\text{[α]}}_{\text{D}}^{\text{25}}$ = −3.2 (c = 0.01, MeOH); CD (MeOH; c 0.2): Δε210 −0.23; ¹H and ¹³C NMR data: see Table 2; ESI-MS m/z 291 [M + Na]⁺, 559 [2M + Na]⁺.

Sinulolide D (4): colorless oil; ${\text{[α]}}_{\text{D}}^{\text{25}}$ = −2.5 (c = 0.01, MeOH); CD (MeOH; c 0.2): Δε207 –0.48, Δε238 −0.46; ¹H and ¹³C NMR data: see Table 2; ESI-MS m/z 253 [M – H]⁻.

Sinulolide E (5): colorless oil; ${\text{[α]}}_{\text{D}}^{\text{25}}$ = −3.8 (c = 0.01, MeOH); CD (MeOH; c 0.2): Δε218 –0.52; ¹H and ¹³C NMR data: see Table 2; ESI-MS m/z 299 [M + H]⁺, 321 [M + Na]⁺.

Sinulolide F (6): colorless oil; ${\text{[α]}}_{\text{D}}^{\text{25}}$ = −6.6 (c = 0.01, MeOH); CD (MeOH; c 0.2): Δε206 −0.48; ¹H and ¹³C NMR data: see Table 2; ESI-MS m/z 283 [M − H] ⁻.

Sinulolide G (7): colorless oil; ${\text{[α]}}_{\text{D}}^{\text{25}}$ = −4.4 (c = 0.05, MeOH); CD (MeOH; c 0.2): Δε229 −0.4; ¹H NMR (500 MHz, CDCl₃) δ: 3.66 (3H, s), 3.08 (3H, s), 2.29 (2H, m), 1.99 (1H, m), 1.85 (6H, s), 1.70 (1H, m), 1.61 (2H, m), 1.23 (2H, m); ¹³C NMR (125 MHz, CDCl₃) δ: 173.4 (C, C-9), 171.9 (C, C-1), 155.6 (C, C-3), 127.4 (C, C-2), 109.5 (C, C-4), 51.6 (CH₃, C-10), 50.1 (CH₃, C-11), 35.3 (CH₂, C-5), 33.8 (CH₂, C-8), 24.8 (CH₂, C-6), 22.3 (CH₂, C-7), 10.7 (CH₃, C-13), 8.5 (CH₃, C-12); ESI-MS m/z: 279 [M + Na]⁺, 535 [2M + Na]⁺.

Sinulolide H (8): colorless oil; ${\text{[α]}}_{\text{D}}^{\text{25}}$ = −3.2 (c = 0.03, MeOH); CD (MeOH; c 0.2): Δε229 −0.4; ¹H NMR (500 MHz, CDCl₃) δ: 3.66 (3H, s), 3.07 (3H, s), 2.52 (1H, m), 2.39 (1H, m), 2.30 (1H, m), 1.96 (1H, m), 1.88 (3H, s), 1.85 (3H, s); ¹³C NMR (125 MHz, CDCl₃) δ: 173.2 (C, C-7), 171.2 (C, C-1), 155.7 (C, C-3), 127.7 (C, C-2), 108.9 (C, C-4), 51.8 (CH₃, C-8), 50.2 (CH₃, C-9), 31.2 (CH₂, C-5), 27.8 (CH₂, C-6), 10.7 (CH₃, C-11), 8.5 (CH₃, C-10); ESI-MS at m/z: 251 [M + Na]⁺, 479 [2M + Na]⁺.

3.4. Assays for Bioactivities

Bioactivity assays were performed by the National Center for Drug Screening, the State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, using HTS [25]. Previously reported procedures were followed for assaying the bioactivity against Foxo3α [26], HMGCR-GFP [27], NF-κB luciferase [28], PGC-1α [29], PTP1B [30], MMP [31] and AMPK [32].

3.5. Computational Calculation

The computational ECD, specific rotation and ¹³C NMR calculations were performed by the B3LYP functional and a generic basis set, employing the 6-311+G (d,p) basis set [21,33]. This generic basis set has been shown to be effective, both efficient and reliable, in predicting structural and reactivity properties for homogeneous systems. Molecular Merck force field (MMFF) and density functional theory/time dependent density functional theory (DFT/TDDFT) calculations were performed with Spartan’14 software package (Wavefunction Inc., Irvine, CA, USA) and the Gaussian 09 program package, respectively, using default grids and convergence criteria.

4. Conclusions

Our study revealed the chemical constituents of soft coral Sinularia sp., which is rich in the South China Sea. Ten compounds were isolated and purified, including seven cyclopentenone derivatives and three butenolide derivatives. Using HTS, their bioactivities toward several targets, such as Foxo3α, HMGCR-GFP, NF-κB-luciferase, PGC-1a, PTP1B, MMP and AMPK, were evaluated. Compounds 5 and 10 exhibited moderate effects for the inhibition of NF-κB activation.