Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds
Abstract
:1. Introduction
2. Results
Strain | Cancer Cell Lines | ||||||||
---|---|---|---|---|---|---|---|---|---|
HepG2 | RKO | MG-63 | SK-BR-3 | T47D | HT-29 | SH-SY5Y | PC-3 | ||
Nodosilinea nodulosa | LEGE 06152 | − | − | − | − | − | +++ | + | +++ |
Leptolyngbya cf. halophila | LEGE 06102 | +++ | + | ++ | − | − | ++ | + | ++ |
Leptolyngbya mycoidea | LEGE 06108 | − | − | − | − | ++ | − | − | − |
Leptolyngbya mycoidea | LEGE 06118 | + | + | ++ | − | ++ | + | ++ | − |
Leptolyngbya mycoidea | LEGE 06009 | − | − | − | − | ++ | +++ | + | ++ |
Leptolyngbya fragilis | LEGE 07167 | ++ | ++ | +++ | ++ | +++ | + | +++ | +++ |
Pseudanabaena aff. curta | LEGE 07160 | − | + | + | − | − | ++ | − | ++ |
Pseudanabaena aff. curta | LEGE 07169 | ++ | − | +++ | + | ++ | +++ | − | +++ |
Pseudanabaena aff. persicina | LEGE 07163 | − | − | − | − | − | − | − | − |
Pseudanabaena sp. | LEGE 06144 | +++ | − | − | +++ | + | − | − | − |
Pseudanabaena sp. | LEGE 06194 | − | − | − | − | − | − | − | − |
Cyanobium sp. | LEGE 06098 | − | − | ++ | + | − | − | ++ | − |
Cyanobium sp. | LEGE 06134 | − | − | + | − | − | + | − | − |
Cyanobium sp. | LEGE 07175 | + | − | ++ | − | ++ | − | − | − |
Cyanobium sp. | LEGE 07186 | − | − | ++ | − | ++ | ++ | − | ++ |
Cyanobium sp. | LEGE 06113 | +++ | − | + | ++ | − | − | ++ | − |
Cyanobium sp. | LEGE 06137 | − | ++ | − | − | − | − | + | − |
Cyanobium sp. | LEGE 06097 | + | − | − | − | ++ | + | − | − |
Cyanobium sp. | LEGE 06139 | − | ++ | − | − | + | − | + | − |
Synechococcus nidulans | LEGE 07171 | + | − | − | +++ | − | − | ++ | − |
Synechococcus sp. | LEGE 07172 | − | ++ | − | − | − | − | ++ | − |
Synechococcus sp. | LEGE 06005 | − | − | ++ | − | − | − | − | − |
Synechococcus sp. | LEGE 06026 | − | − | − | + | − | + | − | − |
Synechocystis salina | LEGE 06099 | +++ | − | ++ | +++ | − | − | + | − |
Synechocystis salina | LEGE 06155 | +++ | +++ | + | ++ | + | + | ++ | − |
Synechocystis salina | LEGE 07173 | + | − | − | − | + | − | ++ | − |
Romeria sp. | LEGE 06013 | − | ++ | − | − | − | − | + | − |
Romeria aff. gracilis | LEGE 07310 | + | − | + | +++ | ++ | − | ++ | − |
Cell line | Strain | MTT | LDH |
---|---|---|---|
HepG2 | LEGE 06155 | B | B |
LEGE 06099 | A | A | |
LEGE 06102 | A | A | |
LEGE 06113 | B | A | |
LEGE 06144 | Crude | - | |
HT-29 | LEGE 07169 | C | A |
LEGE 06009 | Crude | - | |
LEGE 06152 | B | B | |
MG-63 | LEGE 07169 | B | - |
LEGE 07167 | Crude, A, B, C | - | |
PC-3 | LEGE 07169 | A | - |
LEGE 06009 | A | A | |
LEGE 06152 | Crude, A, B, C | - | |
RKO | LEGE 06155 | B | B |
SH-SY5Y | LEGE 07167 | B | - |
T47D | LEGE 07167 | B | Crude |
3. Discussion
4. Experimental Section
4.1. Cyanobacteria Strains and Culture
Taxon | Code | Sampling Location | Accession Number | Reference | Medium |
---|---|---|---|---|---|
Nodosilinea nodulosa | LEGE 06152 | Lavadores (4) | HQ832915 | [31] | Z8 |
Leptolyngbya cf. halophila | LEGE 06102 | S. Bartolomeu do Mar (2) | HQ832906 | [43] | Z8 |
Leptolyngbya mycoidea | LEGE 06108 | Luz (11) | HQ832942 | [43] | Z8 |
Leptolyngbya mycoidea | LEGE 06118 | Luz (11) | HQ832943 | [43] | Z8 |
Leptolyngbya mycoidea | LEGE 06009 | Foz do Arelho (7) | JF708121 | [43] | Z8 |
Leptolyngbya fragilis | LEGE 07167 | Lavadores (4) | HQ832917 | [43] | MN |
Pseudanabaena aff. curta | LEGE 07160 | Olhos d’Água (12) | HQ832948 | [43] | MN |
Pseudanabaena aff. curta | LEGE 07169 | Aguda (5) | HQ832923 | [43] | MN |
Pseudanabaena aff. persicina | LEGE 07163 | Moledo (1) | HQ832900 | [43] | MN |
Pseudanabaena sp. | LEGE 06144 | Burgau (10) | HQ832937 | [43] | MN |
Pseudanabaena sp. | LEGE 06194 | Luz (11) | - | - | MN |
Cyanobium sp. | LEGE 06098 | Martinhal (9) | KC469572 | [44] | Z8 |
Cyanobium sp. | LEGE 06134 | Moledo (1) | KC469573 | [44] | Z8 |
Cyanobium sp. | LEGE 07175 | Martinhal (9) | KC469575 | [44] | Z8 |
Cyanobium sp. | LEGE 07186 | Martinhal (9) | KC469576 | [44] | Z8 |
Cyanobium sp. | LEGE 06113 | Aguda (5) | KC469577 | [45] | Z8 |
Cyanobium sp. | LEGE 06137 | Lavadores (4) | HQ832914 | [43] | Z8 |
Cyanobium sp. | LEGE 06097 | Martinhal (9) | HQ832928 | [43] | Z8 |
Cyanobium sp. | LEGE 06139 | Aguda (5) | KC469574 | [44] | Z8 |
Synechococcus nidulans | LEGE 07171 | Burgau (10) | HQ832939 | [43] | Z8 |
Synechococcus sp. | LEGE 07172 | Olhos d’Água (12) | HQ832950 | [43] | Z8 |
Synechococcus sp. | LEGE 06005 | São Pedro de Moel (6) | HM124558 | [37] | Z8 |
Synechococcus sp. | LEGE 06026 | Empa (8) | - | - | Z8 |
Synechocystis salina | LEGE 06099 | Moledo (1) | HQ832895 | [43] | Z8 |
Synechocystis salina | LEGE 06155 | S. Bartolomeu do Mar (2) | HQ832911 | [43] | Z8 |
Synechocystis salina | LEGE 07173 | Olhos d’Água (12) | HQ832951 | [43] | Z8 |
Romeria sp. | LEGE 06013 | Foz do Arelho (7) | HQ832927 | [43] | Z8 |
Romeria aff. gracilis | LEGE 07310 | Minho estuary (3) | HM217057 | [46] | Z8 |
4.2. Extract Preparation
4.3. Cell Lines
4.4. Cytotoxicity Assays
4.4.1. MTT Assay
4.4.2. LDH Release Assay
4.5. Statistical Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Costa, M.; Garcia, M.; Costa-Rodrigues, J.; Costa, M.S.; Ribeiro, M.J.; Fernandes, M.H.; Barros, P.; Barreiro, A.; Vasconcelos, V.; Martins, R. Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds. Mar. Drugs 2014, 12, 98-114. https://doi.org/10.3390/md12010098
Costa M, Garcia M, Costa-Rodrigues J, Costa MS, Ribeiro MJ, Fernandes MH, Barros P, Barreiro A, Vasconcelos V, Martins R. Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds. Marine Drugs. 2014; 12(1):98-114. https://doi.org/10.3390/md12010098
Chicago/Turabian StyleCosta, Margarida, Mónica Garcia, João Costa-Rodrigues, Maria Sofia Costa, Maria João Ribeiro, Maria Helena Fernandes, Piedade Barros, Aldo Barreiro, Vitor Vasconcelos, and Rosário Martins. 2014. "Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds" Marine Drugs 12, no. 1: 98-114. https://doi.org/10.3390/md12010098
APA StyleCosta, M., Garcia, M., Costa-Rodrigues, J., Costa, M. S., Ribeiro, M. J., Fernandes, M. H., Barros, P., Barreiro, A., Vasconcelos, V., & Martins, R. (2014). Exploring Bioactive Properties of Marine Cyanobacteria Isolated from the Portuguese Coast: High Potential as a Source of Anticancer Compounds. Marine Drugs, 12(1), 98-114. https://doi.org/10.3390/md12010098