Isolation and Characterization of Collagen and Antioxidant Collagen Peptides from Scales of Croceine Croaker (Pseudosciaena crocea)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Collagen
2.1.1. Proximate Analysis
2.1.2. Amino Acid Composition
Amino Acid | ASC-C | CSC | Amino Acid | ASC-C | CSC |
---|---|---|---|---|---|
Hyp | 79.4 | 95.1 | Leu | 23.9 | 23.4 |
Asp | 40.7 | 45.7 | Tyr | 4.1 | 3.7 |
Thr | 24.2 | 18.4 | Phe | 15.2 | 3.3 |
Ser | 30.5 | 33.2 | Hyl | 5.5 | 7.7 |
Glu | 64.7 | 75.9 | Lys | 24.8 | 26.5 |
Pro | 110.0 | 121.5 | His | 8.2 | 5.3 |
Gly | 347.1 | 330.6 | Arg | 44.9 | 51.0 |
Ala | 124.5 | 119.7 | Met | 13.2 | 6.1 |
Cys | 3.2 | 0.0 | Trp | 0.0 | 0.0 |
Val | 24.5 | 21.5 | Imino acid | 189.4 | 216.6 |
Ile | 11.4 | 11.4 | Total | 1000.0 | 1000.0 |
2.1.3. Electrophoretic Pattern of ASC-C
2.2. Isolation of Antioxidant Peptides from ASC-C
2.2.1. Double-Enzyme Hydrolysis and Preparation of Hydrolysate of ASC-C (ACH)
Hydrolysates | DH (%) | Hydroxyl Radical Scavenging Activity (%)(c = 10 mg/mL) |
---|---|---|
Trypsin | 17.47 ± 0.63 | 53.11 ± 0.97 |
Pepsin | 13.73 ± 0.83 | 44.96 ± 1.97 |
DE-1 | 20.28 ± 1.05 | 62.33 ± 3.01 |
DE-2 | 19.67 ± 0.68 | 60.24 ± 3.38 |
DE-3 | 25.11 ± 0.67 | 85.92 ± 3.84 |
DE-4 | 22.61 ± 0.74 | 65.46 ± 2.56 |
2.2.2. Fractionation of ACH by Ultrafiltration
2.2.3. Gel Filtration Chromatography of ACH-III
2.2.4. Isolation Peptides from ACH-III-3 by RP-HPLC
2.3. Amino Acid Sequence Analysis and Molecular Mass Determination
2.4. Antioxidative Activity of ACH-P1, ACH-P2, and ACH-P3
2.4.1. Hydroxyl Radical Scavenging Activity
2.4.2. DPPH Radical Scavenging Activity
2.4.3. Superoxide Anion Radical Scavenging Activity
2.4.4. ABTS Radical Scavenging Activity
2.4.5. Lipid Peroxidation Inhibition Assay
2.4.6. Relationship between Antioxidant Activities and Amino Acid Compositions of Peptides
3. Experimental Section
3.1. Materials
3.2. Preparation of Collagen from Scales
3.3. Characterization of Collagen
3.3.1. Proximate Analysis
3.3.2. Determination of Amino Acid Composition
3.3.3. SDS-polyacrylamide Gel Electrophoresis (SDS-PAGE) of ASC-C
3.4. Isolation of Antioxidant Peptides from ASC-C
3.4.1. Double-Enzyme Hydrolysis and Preparation of Hydrolysate of ASC-C (ACH)
3.4.2. Fractionation of ACH by Ultrafiltration
3.4.3. Gel Filtration Chromatography of ACH-III
3.4.4. Isolation Peptides from ACH-III-3 by RP-HPLC
3.5. Characterization of Collagen Peptides
3.5.1. Degree of Hydrolysis (DH)
3.5.2. Amino Acid Sequence Analysis and Molecular Mass Determination
3.5.3. Antioxidative Activity
3.5.3.1. Hydroxyl Radical Scavenging Activity
3.5.3.2. DPPH Radical Scavenging Activity
3.5.3.3. Superoxide Anion Radical Scavenging Activity
3.5.3.4. ABTS Radical Scavenging Activity
3.5.3.5. Lipid Peroxidation Inhibition Assay
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Wang, B.; Wang, Y.-M.; Chi, C.-F.; Luo, H.-Y.; Deng, S.-G.; Ma, J.-Y. Isolation and Characterization of Collagen and Antioxidant Collagen Peptides from Scales of Croceine Croaker (Pseudosciaena crocea). Mar. Drugs 2013, 11, 4641-4661. https://doi.org/10.3390/md11114641
Wang B, Wang Y-M, Chi C-F, Luo H-Y, Deng S-G, Ma J-Y. Isolation and Characterization of Collagen and Antioxidant Collagen Peptides from Scales of Croceine Croaker (Pseudosciaena crocea). Marine Drugs. 2013; 11(11):4641-4661. https://doi.org/10.3390/md11114641
Chicago/Turabian StyleWang, Bin, Yu-Mei Wang, Chang-Feng Chi, Hong-Yu Luo, Shang-Gui Deng, and Jian-Yin Ma. 2013. "Isolation and Characterization of Collagen and Antioxidant Collagen Peptides from Scales of Croceine Croaker (Pseudosciaena crocea)" Marine Drugs 11, no. 11: 4641-4661. https://doi.org/10.3390/md11114641
APA StyleWang, B., Wang, Y.-M., Chi, C.-F., Luo, H.-Y., Deng, S.-G., & Ma, J.-Y. (2013). Isolation and Characterization of Collagen and Antioxidant Collagen Peptides from Scales of Croceine Croaker (Pseudosciaena crocea). Marine Drugs, 11(11), 4641-4661. https://doi.org/10.3390/md11114641