Antioxidant and Anti-Protease Activities of Diazepinomicin from the Sponge-Associated Micromonospora Strain RV115
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cytotoxicity of Diazepinomicin in Human Kidney Cells (HK-2)
2.2. Antioxidant Potential of Diazepinomicin
2.3. Protective Effect of Diazepinomicin against Oxidative Stress-Induced Cell Death in HK-2 Cells
Test substance | Cell death (% dead cells) |
---|---|
Control | 4 ± 1.0 |
25 µM DZP | 4.5 ± 0.0 (Δ vs. control ) |
50 µM tempol | 9.7 ± 4.3 |
100 µM H2O2 | 21 ± 5.8 (* vs. control) |
100 µM H2O2 + 2 µM DZP | 6.5 ± 4.4 (≠ vs. H2O2) |
100 µM H2O2 + 5 µM DZP | 7.5 ± 1.8 (≠ vs. H2O2) |
100 µM H2O2 + 10 µM DZP | 5.3 ± 1.0 (≠ vs. H2O2) |
100 µM H2O2 + 25 µM DZP | 6.3 ± 2.5 (≠ vs. H2O2) |
100 µM H2O2 + 50 µM tempol | 14.7 ± 5.8 |
2.4. Antitrypanosomal and Anti-Protease Activities
3. Experimental Section
3.1. Cultivation and Purification
3.2. Ferric Reducing Antioxidant Power (FRAP) Assay
3.3. Cell Culture Maintenance
3.4. Flow Cytometric Quantification of Antioxidant Activity
3.5. Vitality Test
3.6. Comet Assay
3.7. Statistics for Cell Based Assays
3.8. Antitrypanosomal Activity
3.9. Protease Assays
4. Conclusions
Acknowledgments
References
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Abdelmohsen, U.R.; Szesny, M.; Othman, E.M.; Schirmeister, T.; Grond, S.; Stopper, H.; Hentschel, U. Antioxidant and Anti-Protease Activities of Diazepinomicin from the Sponge-Associated Micromonospora Strain RV115. Mar. Drugs 2012, 10, 2208-2221. https://doi.org/10.3390/md10102208
Abdelmohsen UR, Szesny M, Othman EM, Schirmeister T, Grond S, Stopper H, Hentschel U. Antioxidant and Anti-Protease Activities of Diazepinomicin from the Sponge-Associated Micromonospora Strain RV115. Marine Drugs. 2012; 10(10):2208-2221. https://doi.org/10.3390/md10102208
Chicago/Turabian StyleAbdelmohsen, Usama Ramadan, Matthias Szesny, Eman Maher Othman, Tanja Schirmeister, Stephanie Grond, Helga Stopper, and Ute Hentschel. 2012. "Antioxidant and Anti-Protease Activities of Diazepinomicin from the Sponge-Associated Micromonospora Strain RV115" Marine Drugs 10, no. 10: 2208-2221. https://doi.org/10.3390/md10102208