Next Article in Journal
Long-Chain Acetylenic Ketones from the Micronesian Sponge Haliclona sp. Importance of the 1-yn-3-ol Group for Antitumor Activity
Previous Article in Journal
Rigidin E, a New Pyrrolopyrimidine Alkaloid from a Papua New Guinea Tunicate Eudistoma Species
Open AccessArticle

Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac

1
State Key Laboratory of Microbial Technology, College of Life Sciences, Shandong University, Jinan 250100, Shandong Province, P. R. China
2
Department of Biochemistry, College of Biological Sciences, The Ohio State University, Columbus, Ohio 43210, USA
*
Author to whom correspondence should be addressed.
Mar. Drugs 2003, 1(1), 34-45; https://doi.org/10.3390/md101034
Received: 10 October 2003 / Accepted: 13 November 2003 / Published: 26 November 2003
CMP-Neu5Ac is an important sugar nucleotide for biosynthesis of sialic acid and its conjugates. In this paper, a large-scale production system of CMP-Neu5Ac by a single strain is reported. The co-expression of Neu5Ac aldolase (EC4.1.3.3) and CMP-Neu5Ac synthetase (EC 2.7.7.43) was achieved by constructing individual genes into one plasmid and having a single culture that has both NeuAc aldolase and CMP-Neu5Ac synthetase activities. Overall this system only employed N-acetylmannosamine, excess of pyruvate and CTP to produce CMP-Neu5Ac. This work has demonstrated that a large-scale synthesis of sialic acid-derived oligosaccharides could be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism. View Full-Text
Keywords: biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase
MDPI and ACS Style

Song, J.; Zhang, H.; Wu, B.; Zhang, Y.; Li, H.; Xiao, M.; Wang, P.G. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Mar. Drugs 2003, 1, 34-45.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop