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Review

Cytochromes P450 of Caenorhabditis elegans: Implication in Biological Functions and Metabolism of Xenobiotics

by
Lucie Larigot
1,
Daniel Mansuy
2,
Ilona Borowski
2,
Xavier Coumoul
1,* and
Julien Dairou
2,*
1
Campus Saint Germain, INSERM UMR-S 1124, Université de Paris, 45 rue des Saints-Pères, 75006 Paris, France
2
Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, CNRS, Université de Paris, 75006 Paris, France
*
Authors to whom correspondence should be addressed.
Biomolecules 2022, 12(3), 342; https://doi.org/10.3390/biom12030342
Submission received: 24 January 2022 / Revised: 17 February 2022 / Accepted: 17 February 2022 / Published: 22 February 2022
(This article belongs to the Special Issue New Insights into Cytochrome P450s)
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Abstract

:
Caenorhabditis elegans is an important model used for many aspects of biological research. Its genome contains 76 genes coding for cytochromes P450 (P450s), and few data about the biochemical properties of those P450s have been published so far. However, an increasing number of articles have appeared on their involvement in the metabolism of xenobiotics and endobiotics such as fatty acid derivatives and steroids. Moreover, the implication of some P450s in various biological functions of C. elegans, such as survival, dauer formation, life span, fat content, or lipid metabolism, without mention of the precise reaction catalyzed by those P450s, has been reported in several articles. This review presents the state of our knowledge about C. elegans P450s.

1. Introduction

The soil nematode Caenorhabditis elegans (C. elegans) is one of the simplest organisms with a laboratory model status. C. elegans is a transparent worm of about 1 mm in length, found in temperate soil environments [1]. C. elegans is an important model used for many aspects of biological research [2]. It is a non-infectious and non-pathogenic organism that survives by feeding on microbes such as bacteria. With abundant food and low population density, C. elegans has a lifespan of around two or three weeks with a generation time average of three and half days. C. elegans’s developmental stages are eggs, larvae, fertile adults, and post-reproductive adults. C. elegans larvae complete development from embryo to adult with four larval stages (L1–L4) in three days. However, in an environment with limited food and/or high population density, larvae may arrest development at L2 to enter a particular stage called the dauer stage (L2d [3]). The dauer larva has a unique morphology with physiology and metabolism, which allow resistance to environmental stress. The dauer larva can live up to several months, and this stage ends when conditions favor further growth of the larva, now into the L4 stage. The dauer stage is considered to be non-aging because the post-dauer life span is not affected by the length of this stage [4]. The C. elegans model proposes the power of integrated whole animal investigations that are cost- and time-efficient and require minimal infrastructure. It is nowadays a powerful model organism not only for developmental biology but also for aging studies or toxicology [2,5]. C. elegans somatic cell locations and types, as well as networks neurons, have been mapped [6], allowing morphological evaluations of abnormalities induced by toxins and deep neurological/behavioral correlations. Furthermore, genes and signaling pathways appear to be well conserved between C. elegans and humans [7,8]. Despite their different complexity, the number of genes in C. elegans and humans is surprisingly similar (~21.000 genes in humans, ~19.000 genes in C. elegans) [9].
Contrary to toxicity tests using cell cultures, C. elegans toxicity tests provide data from a whole animal with intact and metabolically active digestive, reproductive, endocrine, sensory, and neuromuscular functions. Toxicity ranking, including LD50, in this nematode, has repeatedly been shown to be as predictive as the LD50 rankings using rats or mice [5]. However, the defense mechanisms against xenobiotics in C. elegans have been little studied, although they are essential in toxicological studies. The xenobiotic-metabolizing enzymes, and particularly P450-dependent monooxygenases (phase I enzymes), play a key role in these defense mechanisms. In this review, we present the state of our knowledge about C. elegans P450s.
P450s are ubiquitous heme-thiolate proteins involving iron-protoporphyrin IX in their active site that are widely distributed in living organisms [10,11]. The common property to all P450s discovered so far is the peculiar position of the Soret peak of their Fe(II)–CO complex around 450 nm. This redshifted Soret peak is the signature of the presence of a cysteinate ligand from the protein bound to the iron in trans position to the CO ligand [11]. Most P450s catalyze monooxygenase reactions, such as hydroxylations or epoxidations, on a great number of substrates. These reactions require the presence of dioxygen as well as a cofactor, NADPH or NADH, providing the necessary electrons via electron transfer proteins that are very often coupled to P450 inside cell membranes [11]. In the human genome, 57 genes encoding P450s have been identified (drnelson.uthsc.edu (accessed on 24 January 2022)). Some P450s catalyze oxidation steps involved in the biosynthesis and/or biodegradation of endogenous compounds such as steroids, fatty acids, and endocannabinoids [12]. On the other hand, P450s play a key role in the oxidative biotransformation of xenobiotics such as drugs, pesticides, and other environmental chemicals, facilitating their elimination from living organisms [11,12].
We know of over 62,000 bacterial P450s and 85,000 fungal P450s [13]. Since 1989, P450s have been classified according to their degree of identity in their amino acid sequence. Indeed, P450s with a degree of identity greater than 40% belong to the same family and are designated by a number behind the abbreviation P450 or CYP (CYP13, CYP14). When they have a degree of identity greater than 55%, they belong to the same subfamily and are therefore designated by a capital letter behind the number of the family (CYP13A, CYP13B). Finally, isoforms belonging to the same subfamily are differentiated by a number behind the letter of the subfamily (CYP13A1, CYP13A10). Humans have 57 functional P450s classified in 18 families and 43 subfamilies [12,14]. The largest P450s families in humans are 2, 3, and 4, and some of them are even larger in other mammals such as a mouse. As the number of families increased, it was necessary to establish a new reunification. Clans bring together families that belong to the same group (from the same ancestral gene) according to many phylogenetic trees established previously [15]. There are 11 animal P450s clans (clans 2, 3, 4, 7, 19, 20, 26, 46, 51, 74, and mitochondrial or mito). However, not all organisms own all 11 clans. For example, ecdysozoa (insects, crustaceans, nematodes including C. elegans) only have clans 2, 3, 4, and mito [15,16], whereas humans have 10 out of 11 clans, all except clan 74 [17].
The genomic sequence of the nematode C. elegans reveals over 19,000 genes, of which 76 encode for P450s [9]. Studies on this subject, having led to about a hundred articles that will be mentioned below, show that some P450s are involved in the regulation of the transition to the dauer state as well as other physiological functions of the worm. Other P450s are involved in the metabolism and bioactivation or detoxication of xenobiotics. As vertebrates, C. elegans can induce some P450s, in particular through the Aryl hydrocarbon receptor (AhR) signaling pathway. AhR is a receptor well known for its fundamental role in the metabolism of xenobiotics in vertebrates [18]. Ligands of AhR are hydrophobic xenobiotics including polycyclic aromatic hydrocarbons and halogenated compounds such as benzo (a) pyrene and 2,3,7,8-tetrachlorodibenzo-P-dioxin. AhR also has endogenous ligands such as some steroids and kynurenine. Activation of AhR leads to its translocation from the cytoplasm to the nucleus and then its heterodimerization with its partner, the aryl hydrocarbon receptor nuclear translocator (ARNT), thus forming a transcription factor. The heterodimer AhR/ARNT directly regulates the expression of many genes, including those of some enzymes involved in the metabolism of xenobiotics (P450s of family 1 and glutathione-S-transferases) [19].
In the following, we will review what is presently known about C. elegans P450s.

2. Genetic and Phylogenetic Analysis of C. elegans P450s

C. elegans was the first multicellular organism to have its whole genome sequenced [9] and is the only organism to have its connectome (neuronal “wiring diagram”) completed [20]. It contains 82 P450 genes, including 6 pseudogenes, divided into 16 families (13, 14, 22, 23, 25, 29, 31, 32, 33, 34, 35, 36, 37, 42, 43, 44) and 26 subfamilies in accordance with the Nelson’s nomenclature [21]. Table 1 shows all the C. elegans P450s grouped by family. Almost all of the C. elegans P450s families appear to be nematode-specific [22], but they correspond to the clans 2, 3, 4, and mito also found in humans [16].
Some families have only one member (22, 23, 36, 42, 43, and 44) while others are very large, such as family 33 with 18 P450s (Table 1).
To compare the C. elegans and human P450s, a phylogenetic analysis was made. In order to achieve a phylogenetic tree, the sequences of all P450s (humans and C. elegans) were collected from the Uniprot website [23]. All trees were produced thanks to the Phylogeny site [24]: the P450 tree of C. elegans, that of human P450s, then a tree grouping together all these P450s. Subsequently, the creation of a more visual tree was carried out with the Cytoscape software [25].
The resulting phylogenetic tree common to humans and C. elegans P450s, shown in Figure 1, indicates interspecies relationships.
Human P450s belong to 10 different clans, whereas C. elegans P450s only belong to 4 clans. Some sequences of C. elegans P450s are closer to human P450s sequences than to those of other C. elegans P450s: this is particularly the case for P450s 22A1 (clan 2, close to 17A1 and 21A2) and 44A1 (clan mito, close to 24A1). This also occurs the other way around, as for human P450 4V2 (clan 4) that is found on the tree more closely related to C. elegans P450s 29A, 32, and 37 than to other human P450s of the same family (P450s 4B1, 4F22). P450 13A10 is found in clan 3. It belongs to the same clan as P450 3A4, which is one of the most important human P450 quite often involved in the metabolism of xenobiotics [12]. We also distinguish differences in the distribution of P450s in the clans between humans and C. elegans. Indeed, although clans 2 and 3 present a proportion rather like that expected considering the higher number of P450s in C. elegans than in humans, we can notice that only one of the 76 P450s of the nematode has a mitochondrial sequence. The proportion is, on the other hand, very different for humans who have in this clan 7 P450s out of 57 in total. Clan 4 also has a higher percentage of human P450s (12 P450s out of 57 in total) than C. elegans P450s (13 P450s out of 76 in total). In these two species, the largest P450s clan is clan 2, whereas, in insects, clan 3 is the largest one [22].
P450s can also be classified on an even higher level into groups that contain similar P450 clans across animals, plants, and bacteria [26]. P450 families have emerged and been lost during evolution. For example, P450 51A1 (Sterol 14α-demethylase) required for one step of cholesterol synthesis is evolutionarily old and found in bacteria, plants, and humans. However, it is not found in nematodes or insects that do not synthesize cholesterol de novo. Most of the phylogenetically well-preserved P450 families typically have only one or a few members conserved across many species, whereas unstable P450 families have variable numbers of members in different species. It is believed that the stable P450 families are essential in the synthesis or degradation of endogenous substrates, while the highly diverse P450 families metabolize xenobiotics or secondary metabolites [27,28].

3. Implication of P450s in Biological Functions of C. elegans

Most of the physiological roles of P450s in C. elegans have been determined by the invalidation of P450 expression (knock-out worms or knock-out/gene silencing by RNA interference). Thus, the implication of some P450s in the biological functions of C. elegans without mention of the precise enzymatic reactions catalyzed by those P450s has been reported in several articles (Table 2).
The silencing of single P450 genes has been described to cause alterations in survival [34,35,37], life span [30,31,32,33,34,35,36], morphology [36,37,38,39,40,41,62], embryonic development [37,42,43], larval development [35,37,42,44], dauer formation [29,30,31,35,45,46,47,48,49,50], reproduction [31,42,51,52,53], fat content [42,47,48,51,53,54,55,56], or lipid metabolism [32,40,42,47,48,50,51,54,55,57,58,59,60,61].
Molecular analyses from several studies allowed a better characterization of the roles of some P450s. It was proposed that P450-dependent eicosanoids may serve as second messengers in the regulation of pharyngeal pumping and food uptake in C. elegans [63]. Some C. elegans P450s are linked to the metabolism of fatty acid-derived signaling molecules [47,55]. The silencing of P450s 31A2 and 31A3 leads to polarization and osmotic defects and to failures in meiosis and embryonic development [42]. P450s 31A2 and 31A3 also appear to be involved in the biosynthesis of lipids that are essential for the correct formation of eggshells [42]. In addition, P450 31A2 is required for sperm motility [64], and it negatively regulates the synthesis of prostaglandins [51]. Similarly, the P450s of the 35A family regulate the levels of several fatty acids and endocannabinoids in C. elegans [54]. Thus, P450s 35A2 and 35A3 are involved in the production of lipids required for eggshell formation [42]. Moreover, the deletion of P450 35A2 affects the lipid regulation and longevity of C. elegans [32]. It was also shown that the hypoxia inducible factor, HIF, regulates C. elegans stress responses and behavior via the nuclear receptor NHR-46 by targeting the gene coding for P450 36A1 [65]. This suggests that P450 36A1 is involved in the biosynthesis of a hormone ligand for this receptor.
Interestingly, there are several P450s that have been implicated in an adaptive response to changing environmental conditions. For example, Cong et al. found six P450 genes (13A8, 13A11, 14A4, 33C2, 33D3, and 35B2), whose expression levels were very low at pH 6.33, while these genes were significantly upregulated when the pH dropped to 3.13 [66].
Only a small number of reactions catalyzed by C. elegans P450s and involving endogenous substrates have been identified so far (Table 3).
Thus, microsomes from adult worms oxidize eicosapentaenoic acid, EPA (a polyunsaturated fatty acid that acts as a precursor of prostaglandins, eicosanoids, and tromboxanes), with the main formation of 17,18-epoxy-eicosatetraenoic acid, 17,18-EEQ [47]. This oxidation is NADPH, and cytochrome P450 reductase dependent and is inhibited by usual inhibitors of mammalian arachidonic acid-metabolizing P450s. RNAi gene silencing experiments showed that P450s 29A3 and 33E2, which are related to mammalian P450 family 2, are mainly responsible for the oxidation of EPA into 17,18-EEQ [47]. Further studies using C. elegans P450 33E2 and human P450 reductase expressed in insect cells showed that P450 33E2 catalyzes the epoxygenation of EPA with the formation of 17,18-EEQ and the hydroxylation of arachidonic acid, AA, into 19-hydroxy-AA [55]. This article also showed that P450 33E2 is expressed in the C. elegans pharynx and that 17,18-EEQ is a regulator of the C. elegans pharyngeal pumping activity. More recently, it was reported that C. elegans P450 13A12 and P450 reductase co-expressed in insect cells catalyze the epoxygenation of EPA into 17,18-EEQ and of AA into 14,15-epoxy-eicosatrienoic acid [58] (Table 3). The same article showed that 17,18-EEQ increases the C. elegans locomotion activity.
Finally, the most documented C. elegans P450 is called DAF-9 or P450 22A1, with nearly 45 articles that refer to this protein. Indeed, P450 22A1 is involved in several pathways controlling dauer formation [29], life span, and gonadal migration [31,37]. P450 22A1 catalyzes a key step in the biosynthesis of 3-keto-cholestenoic acids, also called dafachronic acids, DAs (Table 3). Those bile acid-like steroids act as ligands of the DAF-12 nuclear receptor that governs C. elegans larval development and adult longevity [37,50,67]. DAF-9 catalyzes the oxidation of a terminal methyl group of the lateral chain of cholest-4-en-3-one or cholest-7-en-3-one with the formation of delta-4- and delta-7-dafachronic acids, respectively (Figure 2) [57,60]. DAF-9 is the equivalent of P450 27A1, the human P450 that catalyzes this oxidation of a terminal methyl group of the lateral chain of 3-keto-steroids in man.
Several redox systems are involved in the transfer of electrons from NADPH or NADH for dioxygen reduction at the P450 active site [68]. In all the above-mentioned oxidations catalyzed by microsomal type P450s, electrons from NADPH should be transferred to the P450 active site by a P450 reductase, analogous to human P450 reductase, that is encoded by the C. elegans emb-8 gene [69]. During embryonic development, emb-8 activity is essential for normal interactions between the pronucleus/centrosome complex and the posterior cortex and, thus, for proper anterior-posterior polarity. Emb-8 is also required for the formation of the secreted eggshell [69]. EMB-8 plays an important role in fatty acid modification. For instance, as mentioned in the previous paragraph, oxidation of EPA to 17,18-EEQ by adult worm microsomes is NADPH and P450 reductase dependent [47], and C. elegans P450 13A12 and P450 reductase co-expressed in insect cells catalyze the oxidation of EPA into 17,18-EEQ and of AA into 14,15-epoxy-eicosatrienoic acid [58].
The nature of the protein(s) responsible for electron transfer to the only C. elegans mitochondrial P450, P450 44A1, is less clear. In C. elegans mitochondrion, the Y62E10A.6 and Y73FBA.27 genes are coding for an adrenodoxin reductase and a ferredoxin, respectively [9]. If P450 44A1 functions as a genuine mitochondrial P450, those proteins would supply electrons to P450 44A1, even though it was argued that C. elegans lacks classical mitochondrial-type P450 [22]. More data on the nature and roles of P450 44A1 are required to answer those questions.

4. P450s of C. elegans and Xenobiotics

4.1. Induction of C. elegans P450s by Xenobiotics

As shown in Table 4, many C. elegans P450s are induced after the exposition of the worm to various xenobiotics. Thus, ethanol acts as an inducer of P450s 13A12, 13B1, 25A1, 25A2, 29A2, 32B1, 33B1, 33C6, 34A4, 4A6, 35A3, 35A5, 35B1, 35B2, 35C1, 36A1 and 37B1 [70,71], and caffeine is an inducer of P450s 13A8, 13A12, 14A1, 14A2, 14A4, 14A5, 32A1, 33C3, 33C4, 33C6, 33C7, 33C9, 33E1, 33E2, 33E3, 33E4, 34A7, 34A9, 35A2, 35A3, 35A4, 35A5, 35B1, 35B2, and 43A1 [72]. Interestingly, drugs such as primaquine and lansoprazole are inducers of several members of family 35 (35A1, A2, A3, A4, A5, B1, B2, and C1) and P450 31A3 [73,74,75], whereas rifampicin is an inducer of P450 13A7 [76]. Many pesticides, such as atrazine, fenitrothion, DDT, dichlorvos, rotenone, and thiabendazole are inducers of members of family 35 [73,74,75,77,78,79], whereas endosulfan, cypermethrin, chlorpyrifos, rotenone, and dichlorvos are inducers of P450 34A9 [77], and glyphosate and paraquat of P450 29A2 [77]. Aromatic and polyaromatic molecules, such as beta-naphthoflavone, fluoranthene, bisphenol A, and polychlorinated biphenyls (PCBs), act as inducers of several members of family 35 [56,73,74,75,80,81,82]. PCBs are anthropogenic molecules that were produced to be used as dielectric and coolant fluids in electrical devices. They are highly stable and therefore can accumulate in fat tissues. PCB 52 is one among many congeners. It is noteworthy that PCB 52 is also an inducer of P450s 34A6, 14A3, and 14A5 [56,75,83]. Ethidium bromide is an inducer of several members of families 33 and 35 [84,85]. Metal ions related to Cd are inducers of P450s 13A4,13A5, 13A6, 13A7, 14A4, 29A2, 33C5, 33C7, 34A9, and 35A2 [86], whereas those related to Zn, Cu, and Al induce P450s 29A2, 34A9, and 35A2 [87]. Other organic compounds have also been described as inducers of several P450s of C. elegans (Table 4) [88,89,90,91,92].
It is also noteworthy that an endogenous molecule such as progesterone acts as an inducer of P450s 25A2, 25A6, 29A2, 37A1, and 37B1 [93].

4.2. Metabolism of Xenobiotics by C. elegans P450s

Several Phase I and Phase II enzymes involved in xenobiotics metabolism in most living organisms, such as P450-dependent monooxygenases, dehydrogenases, UDP-glucuronyl transferases, and glutathione transferases, are present in C. elegans [95]. They are upregulated in long-lived C. elegans dauer larva [35]. As they are involved in the metabolism and elimination of potentially toxic endobiotics and xenobiotics, these data suggested that those compounds may be the major determinants of molecular damages that cause aging in C. elegans [35].
First results showing that C. elegans P450s are responsible for the metabolism of xenobiotics were concerned with a worldwide distributed pollutant, 2,2′,5,5′-tetrachloro-biphenyl, PCB 52 [83]. Metabolism of PCB 52 by C. elegans leads to C3- and C4- and/or C6-hydroxy-PCB 52. Experiments using RNAi and P450- mutant strains showed that P450 34A6 and members of the P450 14A family were involved in these aromatic hydroxylations (Table 5 and Figure 3). A few years later, it was reported that C. elegans hydroxylates thiabendazole in position 5 of its aromatic ring and that P450 35D1 is responsible for this reaction [96] (Table 5 and Figure 3).
Even more recently, a study of the metabolism by C. elegans of a series of drugs well known to be oxidized in humans by P450s of families 1, 2, and 3 was published [97]. Tolbutamide, amitriptyline, dextromethorphan, diclofenac, nifedipine, and clomipramine that are oxidized by P450s of families 2 and 3 in humans were found to undergo similar oxidations by C. elegans. However, phenacetin that is oxidized into paracetamol by P450 1A2 in humans is not oxidized by C. elegans. These data would indicate the lack of equivalents of P450s of family 1 in C. elegans [97]. Experiments of P450 gene inactivation by RNAis showed that tolbutamide hydroxylation by C. elegans is mainly dependent on P450 34A9 and to a lesser extent on P450s 36A1 and 34A10 (Table 5). Those P450s are the homologs of P450s of family 2 (2C8, 2C9, and 2C19) that are responsible for this hydroxylation in humans. However, the oxidative N-demethylation and C-H bond hydroxylation of amitriptyline were found to be catalyzed by several C. elegans P450s are the equivalents of human P450s of families 2,3, and 4, whereas those reactions are mainly catalyzed by P450 2D6 in humans [97].
Other experiments have shown that some P450s play a role in the development of toxic effects of several xenobiotics for C. elegans. Thus, the DNA damages and growth inhibition caused by aflatoxin B1, a mycotoxin, in C. elegans were reported to depend on the P450 reductase of this organism, even though the involved P450s were not determined [98] (Table 6). The toxic effects of fenitrothion for C. elegans also appear to derive from the formation of reactive metabolites mainly by P450 35A2 [79] (Table 6). Quite recent data have shown that the genotoxic effects of benzo(a)pyrene, B(a)P on C. elegans do not depend on P450s equivalent to those of human family 1A that do not seem to exist in C. elegans but depend on P450s 35A2, 35A3, and 35A5 [99].
On the contrary, C. elegans P450s may be involved in the detoxication of some xenobiotics. This is the case of P450 13A7, an equivalent of human P450 3A4, that mitigates tetrabromobisphenol A-induced toxicity for C. elegans [94]. This is also the case of P450s of the 35 family, P450s 35A1, 35A2, 35A4, 35B3, and 35C1, that are responsible for the detoxication of 3-bromopyruvate [100] (Table 6). Finally, it was recently reported that the expression of Zebrafish P450 1A1 in C. elegans protects it from the toxic effects of B(a)P and other polyaromatic hydrocarbons [101].

5. Conclusions

The C. elegans genome contains 76 genes coding for P450s. Phylogenetic analysis shows that they are homologous to mammalian P450s of families 2, 3, and 4. Many compounds, including drugs, pesticides, polyaromatic molecules, metal ions, ethanol, or caffeine, act as inducers of C. elegans P450s (Table 2). Very few data about the biochemical properties of those P450s have been published so far. Thus, no spectroscopic or structural study of those P450s has been reported so far. However, an increasing number of articles have appeared on reactions catalyzed by them in the metabolism of xenobiotics (Table 5 and Table 6 and Figure 3) and of endobiotics such as fatty acid derivatives and steroids (Table 3 and Figure 2). Moreover, the implication of some P450s in various biological functions of C. elegans without mention of the precise reaction catalyzed by those P450s has been reported in several articles (Table 2). These preliminary studies at the biological level may pave the way to decipher the enzymatic reactions catalyzed by these P450s. Invalidation of the expression of several P450s has shown their implication in C. elegans survival, morphology, embryonic development, growth, larval development, dauer formation, life span, reproduction, movement, pharyngeal pumping, fat content, or lipid composition and metabolism. All the data reported so far for each P450 are schematically summarized in Table 7. Moreover, because of the importance of the use of C. elegans as a model in biology and toxicology, more complete knowledge of its P450s appears to be important in the near future.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

This work was supported by INSERM (Institut National de la Santé et de la Recherche Médicale); CNRS (Centre National de la Recherche Scientifique); Université Paris Descartes (Bourse de l’école doctorale MTCI). The authors declare they have no actual or potential competing financial interests.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

17:18-EEQ: 17,18-epoxy-eicosatetraenoic acid; AA, arachidonic acid; AhR, aryl hydrocarbon receptor; ARNT, aryl hydrocarbon receptor nuclear translocator; BaP, benzo(a)pyrene; C. elegans, Caenorhabditis elegans; DAs, dafachronic acids; DDT, dichlorodiphenyltrichloroethane; EPA, eicosapentaenoic acid; KO, knock-out; LD50, lethal dose 50; P450, cytochrome P450; PCB 52, 2,2′,5,5′-tetrachloro-biphenyl.

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Biomolecules 12 00342 g001 550
Figure 1. Phylogenetic tree gathering human and C. elegans P450s. The square boxes represent human P450s, while the filled round boxes represent C. elegans P450s. Different colors are assigned according to the clan to which the CYP450s belong.
Figure 1. Phylogenetic tree gathering human and C. elegans P450s. The square boxes represent human P450s, while the filled round boxes represent C. elegans P450s. Different colors are assigned according to the clan to which the CYP450s belong.
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Figure 2. P450 22A1 (DAF-9) catalyzed oxidation of cholesten-3-ones into dafachronic acids.
Figure 2. P450 22A1 (DAF-9) catalyzed oxidation of cholesten-3-ones into dafachronic acids.
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Figure 3. Oxidations of xenobiotics by C. elegans (arrows indicate the oxidation site(s)).
Figure 3. Oxidations of xenobiotics by C. elegans (arrows indicate the oxidation site(s)).
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Table
Table 1. Genes and pseudogenes (in italics) of P450s in C. elegans.
Table 1. Genes and pseudogenes (in italics) of P450s in C. elegans.
FamilyTranscriptTranscript
Length (nt)		ProteinProtein
Length (aa)
13 T10B9.8.11771CYP-13A1519
T10B9.7.12258CYP-13A2515
T10B9.5.11677CYP-13A3520
T10B9.1.11655CYP-13A4520
T10B9.2.11665CYP-13A5520
T10B9.3.11759CYP-13A6518
T10B9.10.11623CYP-13A7518
T10B9.4.11594CYP-13A8509
T10B9.61423CYP-13A9
ZK1320.4.11680CYP-13A10519
F14F7.2.11653CYP-13A11517
F14F7.3.11726CYP-13A12518
F02C12.5.11652CYP-13B1510
K06G5.2.11786CYP-13B2511
14 K09A11.2.11533CYP-14A1 491
K09A11.3.11535CYP-14A2492
K09A11.4.11541CYP-14A3498
R04D3.1.11549CYP-14A4491
F08F3.7.11561CYP-14A5492
22 T13C5.11999CYP-22A1 (daf-9)572
23 B0304.3.11719CYP-23A1534
25 C36A4.11631CYP-25A1502
C36A4.21570CYP-25A2502
C36A4.31775CYP-25A3502
C36A4.61677CYP-25A4501
F42A6.41506CYP-25A5
K06B9.1708CYP-25A6236
29 C44C10.21515CYP-29A1
T19B10.11733CYP-29A2503
Y38C9B.11743CYP-29A3503
B0331.11682CYP-29A4502
31 C01F6.31389CYP-31A1
H02I12.81631CYP-31A2495
Y17G9B.31597CYP-31A3495
Y62E10A.15b2 31077CYP-31A5308
32 C26F1.21691CYP-32A1529
Y5H2B.51648CYP-32B1516
33 C12D5.71591CYP-33A1492
C25E10.211491CYP-33B1496
C45H4.211739CYP-33C1495
C45H4.17a21585CYP-33C2495
F41B5.41784CYP-33C3500
F44C8.11583CYP-33C4493
F41B5.3a.11568CYP-33C5494
F41B5.7a11778CYP-33C6494
F41B5.41560CYP-33C7494
R08F11.31667CYP-33C8494
C50H11.151616CYP-33C9496
Y49C4A.91738CYP-33C11495
Y5H2B.612035CYP-33C12426
K05D4.41597CYP-33D1492
Y17D7A.41 21579CYP-33D3495
C49C8.411753CYP-33E1494
F42A9.511687CYP-33E2494
F42A9.4890CYP-33E3236
34 T10H4.101595CYP-34A1504
T10H4.111766CYP-34A2502
C41G6.11481CYP-34A3
T09H2.1.11680CYP-34A4500
B0213.101591CYP-34A5499
B0213.111722CYP-34A6498
B0213.121579CYP-34A7499
B0213.141584CYP-34A8499
B0213.151665CYP-34A9516
B0213.161571CYP-34A10499
35 C03G6.141546CYP-35A1494
C03G6.151588CYP-35A2494
K09D9.2587CYP-35A3494
C49G7.81552CYP-35A4494
K07C6.51727CYP-35A5494
K07C6.41648CYP-35B1499
K07C6.31621CYP-35B2499
K07C6.21500CYP-35B3499
C06B3.31534CYP-35C1495
F14H3.101576CYP-35D1499
F14H3.13558CYP-35D2
36 C34B7.31750CYP-36A1493
37 F01D5.91561CYP-37A1508
F28G4.11736CYP-37B1509
42 Y80D3A.51826CYP-42A1511
43 E03E2.11698CYP-43A1526
44 ZK177.51551CYP-44A1489
Table
Table 2. Implication of P450s in biological functions of C. elegans.
Table 2. Implication of P450s in biological functions of C. elegans.
Biological FunctionsP450sRef.
Survival/life span22A1[29,30,31,32,33,34,35,36]
Morphology 22A1, 33E2[37,38,39,40,41]
Embryonic development22A1,31A2, 31A3[37,42,43]
Larval development22A1, 32A1, 32B1, 33B1, 33C1, 33C2, 33D1[35,37,42,44]
Dauer formation13A5, 13A7, 14A1, 14A3, 22A1, 29A3, 32A1, 32B1, 33B1, 33C1, 33C2, 33D1, 33E2, 34A2, 34A5, 34A6, 34A7, 34A8, 34A9, 35B1, 35B2[29,30,31,35,45,46,47,48,49,50]
Reproduction31A2, 31A3, 35A2[31,42,51,52,53]
Fat content29A3, 31A2, 31A3, 33E2, 35A1, 35A2, 35A3, 35A4, 35A5[42,47,48,51,53,54,55,56]
Lipid metabolism13A12, 22A1, 29A3, 31A2, 31A3, 33E2, 35A1, 35A2, 35A4, 35A5, 37B1[32,40,42,47,48,50,51,54,55,57,58,59,60,61]
Table
Table 3. Implication of P450s in the metabolism of endogenous compounds in C. elegans.
Table 3. Implication of P450s in the metabolism of endogenous compounds in C. elegans.
EndobioticsMetabolite(s)Reaction(s)P450(s) InvolvedRef.
EPA17,18-epoxy-eicosatetraenoic acidEpoxidation13A12, 29A3, 33E2[47,55,58]
AA19-hydroxy-AAC-H bond hydroxylation33E2[55]
AA14,15-epoxy-eicosatrienoic acidEpoxidation13A12[58]
Cholesten-3-onesDafachronic acidsOxidation of CH3 to COOH22A1 (DAF-9)[57,60]
Table
Table 4. Induction of C. elegans P450s by xenobiotics.
Table 4. Induction of C. elegans P450s by xenobiotics.
Chemical ClassCompoundsP450s InducedRef.
AlcoholEthanol13A12, 13B1, 25A1, 25A2, 29A2, 32B1, 33B1, 33C6, 34A4, 34A6, 35A3, 35A5, 35B1, 35B2, 35C1, 36A1, 37B1[70,71]
Alkaloid Caffeine 13A8, 13A12, 14A1, 14A2, 14A4, 14A5, 32A1, 33C3, 33C4, 33C6, 33C7, 33C9, 33E1, 33E2, 33E3,33E4, 34A7, 34A9, 35A2, 35A3, 35A4, 35A5, 35B1, 35B2, 43A1[72]
Aromatic compoundEthidium bromide, 2,2′,5,5′-tetrachlorobiphenyl (PCB52)13A7, 14A3, 14A5, 33C3, 33C4, 33C5, 33C6, 33C7, 33D3, 34A6, 35A1, 35A2, 35A3, 35A4, 35A5, 35B1, 35B2, 35B3, 35C1[56,73,74,83,84,85]
DrugRifampicine, Lansoprazole, primaquine, phenobarbital13A7, 31A3, 35A1, 35A2, 35A3, 35A4, 35A5, 35C1[73,74,75,76]
HormoneProgesterone, 17-β-estradiol25A2, 25A6, 29A2, 37A1, 37B1[93]
Metal ionZinc, mercury, copper, arsenic, aluminum, cadmium13A4, 13A5, 13A6, 13A7, 14A4, 29A2, 33C5, 33C7, 34A9, 35A2[86,87]
NanoparticleSilver and titane nanoparticles35A2[53,89]
Organic compoundAcrylamide, pyrazole13A12; 31A1, 31A3[73,90]
PesticideGlyphosate, paraquat, endosulfan, cyperméthrine, chlorpyrifos, dichlorvos, dichlorodiphenyltrichloroethane (DDT), pyridazine, rotenone, atrazine, fenitrothion, thiabendazole22A1, 29A2, 34A9, 35A1, 35A2, 35A5, 35B1, 35B2, 35B3, 35C1, 35D1[73,74,75,77,78,79]
PhtalateDiethylhexylphtalate (DEHP)35A2[91]
Polycyclic aromatic hydrocarbonsBeta-naphthoflavone, fluoranthene14A5, 35A1, 35A2, 35A3, 35A4, 35A5, 35B1, 35B2, 35C1, 37B1[73,74,75]
Phenol/PolyphenolBisphenol A, tetrabromobisphenol A, resveratrol13A6, 13A7, 35A2[80,81,82,92,94]
Table
Table 5. Metabolism of xenobiotics by C. elegans: implication of P450s.
Table 5. Metabolism of xenobiotics by C. elegans: implication of P450s.
XenobioticMetabolite(s)Reaction(s)P450(s) InvolvedRef.
2,2′,5,5′-tetrachlorobiphenyl (PCB52)3-,4- or 6- hydroxy-PCB52Aromatic hydroxylations14A members, 34A6[83]
Thiabendazole (TB)5-hydroxy-TBAromatic hydroxylation35D1[96]
Tolbutamide (TA)Hydroxy-TAC-H bond hydroxylation34A9, 34A10 and 36A1[97]
AmitriptylineNortriptyline
E-10-hydroxyamitriptyline		Oxidative N-demethylation
C-H bond hydroxylation		Many P450s
n.d.		[97]
DextromethorphanDextrorphan
3-methoxymorphinan		O-demethylation
N-demethylation		Many P450s
n.d.		[97]
Diclofenac4′-hydroxy-diclofenacAromatic hydroxylationn.d.[97]
NifedipineOxidized nifedipineDehydrogenationn.d.[97]
ClomipramineNorclomipramineN-demethylationn.d.[97]
n.d. not determined.
Table
Table 6. Implication of P450s in the biological effects of xenobiotics on C. elegans.
Table 6. Implication of P450s in the biological effects of xenobiotics on C. elegans.
XenobioticBiological Effect(s)P450(s) InvolvedP450 RoleRef.
Aflatoxine B1DNA damage, growth inhibitionn.d. P450 reductase involvedToxic activation[98]
Tetrabromobisphenol AToxicity13A7Detoxication[94]
3-bromopyruvateToxicity35A1, 35A2, 35A4, 35B3, 35C1Detoxication[100]
FenitrothionToxicity35A2Toxic activation[79]
Benzo(a)pyreneToxicity35A2, 35A3, 35A5Toxic activation[99]
n.d. not determined.
Table
Table 7. Data presently reported on each C. elegans P450.
Table 7. Data presently reported on each C. elegans P450.
FamilyP450sEndogenous Function(s)InducersXenobiotic(s) MetabolizedRef.
13 13A1 ----
13A2 ----
13A3 ----
13A4 -Cadmium-[86,102]
13A5 Dauer larvae and long-lived stateCadmium-[35,86]
13A6 -Cadmium
Resveratrol		-[86,92]
13A7 Dauer larvae and long-lived stateEthidium
Bromide
Cadmium
Rifampicine		Tetrabromobisphenol A[35,76,84,86,94]
13A8 -Caffeine -[72]
13A10 ----
13A11 ----
13A12 Lipid metabolism/fat contentAcrylamide
Caffeine
Ethanol		-[58,71,72,90]
13B1 -Ethanol-[71]
13B2 ----
14 14A1 Dauer larvae and long-lived stateCaffeine PCB52[35,72,83]
14A2 -Caffeine PCB52[72,83]
14A3 Dauer larvae and long-lived statePCB52PCB52[35,56,83]
14A4 -Caffeine
Cadmium		PCB52[72,83,86]
14A5 -Beta-naphthoflavone
Caffeine
PCB52		PCB52[72,75,83]
22 22A1 (DAF-9) Survival/life span
morphology
Embryonic development
Larval development/dauer formation
Lipid metabolism/fat content		Atrazine-[29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,55,57,60,62,75]
23 23A1 ----
25 25A1 -Ethanol-[71]
25A2 -Ethanol
Progesterone		-[70,71,93]
25A3 ----
25A4 ----
25A6 -Progesterone-[93]
29 29A1 ----
29A2 -Ethanol
Progesterone
Aluminum, cadmium, copper, zinc
Glyphosate, paraquat, dichlorvos, rotenone		-[71,77,86,93]
29A3 Dauer larvae
Lipid metabolism/fat content		--[47]
29A4 ----
31 31A2 Embryonic development
Reproduction
Lipid metabolism/fat content		--[35,48,97,103]
31A3 Embryonic development
Lipid metabolism/fat content		Atrazine
Lansoprazole, phenobarbital, primaquine
Pyrazole, toluene		-[73,97,103]
31A5 ---
32 32A1 Larval developmentCaffeine -[35,72]
32B1 Larval development/dauer formationEthanol-[35,70]
33 33A1 ----
33B1 ----
33C1 Larval development/dauer formation--[35]
33C2 Larval development/dauer formation--[35]
33C3 -Caffeine
Ethidium bromide		-[72,84]
33C4 -Caffeine
Ethidium bromide		-[72,84]
33C5 -Cadmium
Ethidium bromide		-[84,86]
33C6 -Caffeine
Ethanol
Ethidium bromide		-[70,72,84]
33C7 -CadmiumCaffeine
Ethidium bromide		-[70,72,84]
33C8 ----
33C9 -Caffeine -[72]
33C11 ----
33C12 ----
33D1 Larval development/dauer formation--[35]
33D3 -Ethidium bromide-[84]
33E1 -Caffeine -[72]
33E2 -Caffeine -[72]
33E3 Morphology
Dauer formation
Lipid metabolism/fat content		Caffeine -[35,47,48,55,72]
33E4 -Caffeine -[72]
34 34A1 ----
34A2 Dauer formation--[35]
34A4 -Ethanol-[70]
34A5 Dauer formation--[35]
34A6 Dauer formationEthanolPCB52[35,70,83]
34A7 Dauer formationCaffeine -[35,72]
34A8 Dauer formation--[35]
34A9 Dauer formationCaffeine
Arsenic, cadmium, nickel, zinc		Tolbutamide[35,72,77,86,97]
34A10 --Tolbutamide[97]
35 35A1 Lipid metabolism/fat contentAtrazine
Beta-naphthoflavone, fluoranthene
Caffeine
Ethidium bromide, PCB52
Lansoprazole, primaquine		3-bromopyruvate[54,56,73,74,75,84,100]
35A2 Reproduction
Lipid metabolism/fat content		Aluminum, arsenic, cadmium, copper, mercury, zincAtrazine, dichlorvos, dichlorodiphenyltrichloroethane (DDT), rotenone, fenitrothion
Beta-naphthoflavone, fluoranthene
Bisphenol A
CaffeineEthidium bromide, PCB52
N-Nitrosodiethylamine (NDMA), dibromoacetic acid (DBAA), Lansoprazole, primaquine
Silver and titane nanoparticles		3-bromopyruvate, fenitrothion,
B(a)P		[32,53,54,56,72,73,74,77,79,80,81,82,86,87,89,91,100]
35A3 Lipid metabolism/fat contentBeta-naphthoflavone
Caffeine
Ethanol
PCB52
Lansoprazole, primaquine		B(a)P[56,71,72,73,84]
35A4 Lipid metabolism/fat contentBeta-naphthoflavone
Caffeine
Lansoprazole, primaquine
PCB52		3-bromopyruvate[56,72,73,100]
35A5 Lipid metabolism/fat contentAtrazine
Beta-naphthoflavone, fluoranthene
Caffeine
Ethanol
Ethidium bromide, PCB52
Lansoprazole, primaquine		B(a)P[56,71,72,73,74,75,84]
35B1 Dauer formationAtrazine
Beta-naphthoflavone
Caffeine
Ethanol
Ethidium bromide
Lansoprazole		-[35,71,72,73,75,84]
35B2 Dauer formationAtrazine
Beta-naphthoflavone
Caffeine
Ethanol
Ethidium bromide
Lansoprazole, primaquine		-[35,71,72,73,75,84,85]
35B3 -Ethidium bromide3-bromopyruvate[84,100]
35C1 -Atrazine
Beta-naphthoflavone, fluoranthene
EthanolLansoprazole, primaquine
PCB52		3-bromopyruvate[71,73,74,75,100]
35D1 -ThiabendazoleThiabendazole[78,96]
36 36A1 -EthanolTolbutamide[71,97]
37 37A1 Lipid metabolism/fat contentProgesterone-[93,104]
37B1 Lipid metabolism/fat contentEthanol
Fluoranthene
Progesterone, 17-ß-estradiol		-[59,70,75,93]
42 42A1 ----
43 43A1 -Caffeine -[72]
44 44A1 ----
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Larigot, L.; Mansuy, D.; Borowski, I.; Coumoul, X.; Dairou, J. Cytochromes P450 of Caenorhabditis elegans: Implication in Biological Functions and Metabolism of Xenobiotics. Biomolecules 2022, 12, 342. https://doi.org/10.3390/biom12030342

AMA Style

Larigot L, Mansuy D, Borowski I, Coumoul X, Dairou J. Cytochromes P450 of Caenorhabditis elegans: Implication in Biological Functions and Metabolism of Xenobiotics. Biomolecules. 2022; 12(3):342. https://doi.org/10.3390/biom12030342

Chicago/Turabian Style

Larigot, Lucie, Daniel Mansuy, Ilona Borowski, Xavier Coumoul, and Julien Dairou. 2022. "Cytochromes P450 of Caenorhabditis elegans: Implication in Biological Functions and Metabolism of Xenobiotics" Biomolecules 12, no. 3: 342. https://doi.org/10.3390/biom12030342

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