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Volume 47
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Article

Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling

by
Ju-Sheng Shieh
1,†,
Yu-Tang Chin
1,†,
Tsu-Te Yeh
2,3,
Jiong Jiong Guo
4,5,
Fung-Wei Chang
6,
Hui-Rong Cheng
1,
Hung-Han Hsu
4,
Wei-Lun Huang
7,
Han-Hsiang Huang
7,
Ya-Yu Hsieh
8,
Chien-Ping Chiang
9,* and
Shih-Ching Wang
10,*
1
Department of Periodontology, School of Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, Taiwan
2
Department of Orthopaedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, Taiwan
3
Medical 3D Printing Center, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, Taiwan
4
Department of Orthopedics and Sports Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
5
MOE China-Europe Sports Medicine Belt and Road Joint Laboratory, Soochow University, Suzhou 215006, China
6
Department of Obstetrics and Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, Taiwan
7
Department of Research and Development, Ascension Medical Biotechnology Co., Ltd., Taipei 114731, Taiwan
8
Department and Graduate Institute of Pharmacology, College of Medicine, National Taiwan University, Taipei 110225, Taiwan
9
Department of Dermatology, Tri-Service General Hospital, National Defense Medical Center, Taipei 114202, Taiwan
10
Department of Dermatology, Saint Paul’s Hospital, Taoyuan 330049, Taiwan
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Curr. Issues Mol. Biol. 2025, 47(7), 539; https://doi.org/10.3390/cimb47070539
Submission received: 4 June 2025 / Revised: 3 July 2025 / Accepted: 9 July 2025 / Published: 11 July 2025
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Small extracellular vesicles (sEVs) derived from mesenchymal stem cells have emerged as promising therapeutic agents in regenerative dermatology. This study evaluated the safety and efficacy of Bio-Pulsed avian mesenchymal stem cell-derived sEVs (AMSC-sEVs), topically applied for hair follicle stimulation and skin rejuvenation. Two prospective, single-arm clinical trials were conducted: one involving 30 participants using a hair ampoule over 60 days, and the other involving 30 participants applying a facial essence for 28 days. Objective measurements demonstrated significant improvements in the anagen/telogen hair ratio, reduced shedding, increased collagen density, and reduced wrinkle depth and pigmentation. Small RNA sequencing and qPCR profiling confirmed that Bio-Pulsed AMSC-sEVs were enriched with regenerative microRNAs, such as miR-21-5p and miR-199a-5p, associated with anti-inflammatory and anti-aging effects. No adverse events were reported. These findings suggest that Bio-Pulsed AMSC-sEVs may offer a safe, non-invasive, and cell-free approach to enhance skin and hair regeneration in human subjects.
Keywords: extracellular vesicles; Bio-Pulsed priming; avian mesenchymal stem cells; regenerative dermatology; hair follicle activation; skin rejuvenation; clinical trial; microRNA profiling; non-invasive therapy; collagen biosynthesis extracellular vesicles; Bio-Pulsed priming; avian mesenchymal stem cells; regenerative dermatology; hair follicle activation; skin rejuvenation; clinical trial; microRNA profiling; non-invasive therapy; collagen biosynthesis
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MDPI and ACS Style

Shieh, J.-S.; Chin, Y.-T.; Yeh, T.-T.; Guo, J.J.; Chang, F.-W.; Cheng, H.-R.; Hsu, H.-H.; Huang, W.-L.; Huang, H.-H.; Hsieh, Y.-Y.; et al. Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling. Curr. Issues Mol. Biol. 2025, 47, 539. https://doi.org/10.3390/cimb47070539

AMA Style

Shieh J-S, Chin Y-T, Yeh T-T, Guo JJ, Chang F-W, Cheng H-R, Hsu H-H, Huang W-L, Huang H-H, Hsieh Y-Y, et al. Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling. Current Issues in Molecular Biology. 2025; 47(7):539. https://doi.org/10.3390/cimb47070539

Chicago/Turabian Style

Shieh, Ju-Sheng, Yu-Tang Chin, Tsu-Te Yeh, Jiong Jiong Guo, Fung-Wei Chang, Hui-Rong Cheng, Hung-Han Hsu, Wei-Lun Huang, Han-Hsiang Huang, Ya-Yu Hsieh, and et al. 2025. "Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling" Current Issues in Molecular Biology 47, no. 7: 539. https://doi.org/10.3390/cimb47070539

APA Style

Shieh, J.-S., Chin, Y.-T., Yeh, T.-T., Guo, J. J., Chang, F.-W., Cheng, H.-R., Hsu, H.-H., Huang, W.-L., Huang, H.-H., Hsieh, Y.-Y., Chiang, C.-P., & Wang, S.-C. (2025). Topical Application of Bio-Pulsed Avian MSC-Derived Extracellular Vesicles Enhances Hair Regrowth and Skin Rejuvenation: Evidence from Clinical Evaluation and miRNA Profiling. Current Issues in Molecular Biology, 47(7), 539. https://doi.org/10.3390/cimb47070539

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