Inhibition of TNF-α-Induced Collagen Degradation and Oxidative Damage by Centipeda minima and Brevilin A in Human Dermal Fibroblasts
Abstract
1. Introduction
2. Materials and Methods
2.1. Plant Materials and Preparation of CMX
2.2. Cell Culture and Sample Preparation
2.3. Cell Viability
2.4. Measurement of Intracellular ROS
2.5. Measurement of Nitric Oxide (NO) Production
2.6. Measurement of Protein Secretion
2.7. Western Blotting
2.8. Statistical Analysis
3. Results
3.1. Content of Brevilin A in CMX
Effects of CMX and Brevilin A on Intracellular ROS Accumulation and MMP-1 and COLIA1 Secretion in TNF-α-Stimulated NHDFs
3.2. Effects of CMX and Brevilin A on NO and PGE2 Overproduction in TNF-α-Stimulated NHDFs
3.3. Effects of Brevilin A on TNF-α-Stimulated Phosphorylation of Mitogen-Activated Protein Kinases (MAPKs) in NHDFs
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
COLIA1 | Collagen Type I Alpha 1 |
COX-2 | Cyclooxygenase 2 |
DCFDA | 2′,7′-Dichlorodihydrofluorescein Diacetate |
ERK | Extracellular Signal-Regulated Kinase |
iNOS | Inducible Nitric Oxide Synthase |
JNK | Jun N-terminal Kinase |
MAPKs | Mitogen-Activated Protein Kinases |
MMP-1 | Matrix Metallopeptidase 1 |
NHDFs | Normal Human Dermal Fibroblasts |
NO | Nitric Oxide |
P38 | p38 Mitogen-Activated Protein Kinase |
PGE2 | Prostaglandin-E2 |
ROS | Reactive Oxygen Species |
TNF-α | Tumor Necrosis Factor-Alpha |
UV | Ultraviolet |
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Lee, S.; Kim, B.H.; Choi, Y.J.; Kim, D.-W.; Cho, E.; Kang, M.; Kim, D.; Pyo, J.; Kang, K.S. Inhibition of TNF-α-Induced Collagen Degradation and Oxidative Damage by Centipeda minima and Brevilin A in Human Dermal Fibroblasts. Curr. Issues Mol. Biol. 2025, 47, 376. https://doi.org/10.3390/cimb47050376
Lee S, Kim BH, Choi YJ, Kim D-W, Cho E, Kang M, Kim D, Pyo J, Kang KS. Inhibition of TNF-α-Induced Collagen Degradation and Oxidative Damage by Centipeda minima and Brevilin A in Human Dermal Fibroblasts. Current Issues in Molecular Biology. 2025; 47(5):376. https://doi.org/10.3390/cimb47050376
Chicago/Turabian StyleLee, Sullim, Byoung Ha Kim, Yea Jung Choi, Dong-Wook Kim, Eunsu Cho, Moonseok Kang, Doeun Kim, Jaesung Pyo, and Ki Sung Kang. 2025. "Inhibition of TNF-α-Induced Collagen Degradation and Oxidative Damage by Centipeda minima and Brevilin A in Human Dermal Fibroblasts" Current Issues in Molecular Biology 47, no. 5: 376. https://doi.org/10.3390/cimb47050376
APA StyleLee, S., Kim, B. H., Choi, Y. J., Kim, D.-W., Cho, E., Kang, M., Kim, D., Pyo, J., & Kang, K. S. (2025). Inhibition of TNF-α-Induced Collagen Degradation and Oxidative Damage by Centipeda minima and Brevilin A in Human Dermal Fibroblasts. Current Issues in Molecular Biology, 47(5), 376. https://doi.org/10.3390/cimb47050376