A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Line and Cell Culture
2.2. Identification and Structural Analysis of Trp-Arg Peptides Derived from Honey Bee DNA
2.3. MTT Assay
2.4. The Formation of Prostate Cancer Spheroids and Three-Dimensional (3D) Culture Assay
2.5. Flow Cytometry
2.6. Quantitative Real-Time PCR
2.7. Western Blot
2.8. JC-1 Staining
2.9. Statistical Analysis
3. Results
3.1. N0820 and N0821 Had Antiproliferation Activity against Androgen-Independent Prostate Cancer Cells
3.2. N0820 and N0821 Suppressed the Prostate Cancer Spheroid Size in a 3D Environment
3.3. N0820 and N0821 Had Ion-Channel-Like Activity and Overloaded Ca2+ into the Mitochondria by Reducing the Expression and Phosphorylation of p53
3.4. N0820 and N0821 Induced Apoptosis in the Androgen-Independent Prostate Cancer Cell Line DU145
3.5. N0820 and N0821 Induced S/G2 Phase Cell Cycle Arrest in the Androgen-Independent Prostate Cancer Cell Line DU145
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Bee Species | Trp-Arg Rich Region in Bee Sequence |
---|---|
Apis cerana | RRSWWCCCWYWWWWRWWRKILGRARWKCRR |
Apis dorsata | RRSWWCCCWYWWWWRWWRKIFGRARWKCRR |
Apis mellifera | RRSWWCCCWYWWWWRWWRKMFGRARWKCRR |
Apis florea | RRSWWCCCWYWWWWRWWRKMFGRARWKCRR |
Bee Species | Species Characteristics |
---|---|
Apis cerana | The species referred to as the Oriental honeybee is predominantly distributed throughout East Asia. Characterized by their relatively smaller size, they predominantly inhabit forested regions. |
Apis dorsata | The species commonly referred to as the giant honeybee is primarily found in Asia and the South Pacific region. These bees are known for their large size and gregarious nature, forming expansive colonies. They typically construct their hives on tree branches, often in large clusters. |
Apis mellifera | The Western honeybee exhibits a global distribution and is recognized as one of the most widely studied species of honey bee. With variations in size, they demonstrate adaptability to diverse environmental conditions and are capable of reproductive success across various habitats. |
Apis florea | The species referred to as the Little Oriental Bee inhabits regions of East and South Asia. It is distinguished by its diminutive size and primarily forages nectar from small flowers. |
Name | Peptide Sequence |
---|---|
N0820 | WWWWRWWRKI |
N0821 | YWWWWRWWRKI |
Gene | Primer Sequence (5′ to 3′) | Annealing Temp. (°C) | References |
---|---|---|---|
GAPDH | F: GTGAAGGTCGGAGTCAACG R: TGAGGTCAATGAAGGGGTC | 57.1 55.3 | [35] |
CDKN2A (p14) | F: GGTTCTTGGTGACCCTC R: CTAGACGCTGGCTCCTCAGT | 59.5 58.4 | [This study] |
CDKN1B (p27) | F: AAGGTTTGGAGAGCGGCTG R: GATCAAATGGACTGGCGAGC | 53.0 52.8 | [This study] |
CCNA2 (Cyclin A2) | F: AGTAAACAGCCTGCGTTCACC R: GAGGGACCAATGGTTTTCTGG | 59.1 57.1 | [36] |
CCNB1 (Cyclin B1) | F: TAAGGCGAAGATCAACATGG R: GCTTCCTTCTTCATAGGCAT | 53.0 52.8 | [37] |
CCND1 (Cyclin D1) | F: CTGTGCTGCGAAGTGGAAACC R: GACGATCTTCCGCATGGAC | 60.6 57.3 | [25] |
CCNE1 (Cyclin E1) | F: ACACCATGAAGGAGGACG R: CACAGACTGCATTATTGTCCC | 55.6 54.5 | [25] |
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Kim, Y.-e.; Kim, K.-Y. A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145. Curr. Issues Mol. Biol. 2024, 46, 2251-2262. https://doi.org/10.3390/cimb46030144
Kim Y-e, Kim K-Y. A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145. Current Issues in Molecular Biology. 2024; 46(3):2251-2262. https://doi.org/10.3390/cimb46030144
Chicago/Turabian StyleKim, Ye-eun, and Ki-Young Kim. 2024. "A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145" Current Issues in Molecular Biology 46, no. 3: 2251-2262. https://doi.org/10.3390/cimb46030144