3,3′,4,5′-Tetramethoxy-trans-stilbene Improves Insulin Resistance by Activating the IRS/PI3K/Akt Pathway and Inhibiting Oxidative Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Cell Culture
2.3. MTT Assay
2.4. Glucose Consumption Assay
2.5. Glycogen Synthesis Assay
2.6. Western Blot Assay
2.7. ROS Assay
2.8. Statistical Analysis
3. Results
3.1. The Effect of 3,3′,4,5′-TMS and DXMS on the HepG2 Cell Viability
3.2. Effect of 3,3′,4,5′-TMS on Glucose Consumption
3.3. 3,3′,4,5′-TMS Enhanced Glycogen Synthesis Ability by Up-Regulating p-GSK3β
3.4. 3,3′,4,5′-TMS Activated IRS/PI3K/Akt Pathway
3.5. 3,3′,4,5′-TMS Alleviated Oxidative Stress by Upregulating Nrf2
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tan, Y.; Miao, L.; Xiao, J.; Cheang, W.S. 3,3′,4,5′-Tetramethoxy-trans-stilbene Improves Insulin Resistance by Activating the IRS/PI3K/Akt Pathway and Inhibiting Oxidative Stress. Curr. Issues Mol. Biol. 2022, 44, 2175-2185. https://doi.org/10.3390/cimb44050147
Tan Y, Miao L, Xiao J, Cheang WS. 3,3′,4,5′-Tetramethoxy-trans-stilbene Improves Insulin Resistance by Activating the IRS/PI3K/Akt Pathway and Inhibiting Oxidative Stress. Current Issues in Molecular Biology. 2022; 44(5):2175-2185. https://doi.org/10.3390/cimb44050147
Chicago/Turabian StyleTan, Yi, Lingchao Miao, Jianbo Xiao, and Wai San Cheang. 2022. "3,3′,4,5′-Tetramethoxy-trans-stilbene Improves Insulin Resistance by Activating the IRS/PI3K/Akt Pathway and Inhibiting Oxidative Stress" Current Issues in Molecular Biology 44, no. 5: 2175-2185. https://doi.org/10.3390/cimb44050147