Next Article in Journal / Special Issue
Resiniferatoxin: The Evolution of the “Molecular Scalpel” for Chronic Pain Relief
Previous Article in Journal / Special Issue
TRPV1 and TRPM8 in Treatment of Chronic Cough
Open AccessReview

Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals

Department of Physiology, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Arpad Szallasi, Susan M. Huang and Jean Jacques Vanden Eynde
Pharmaceuticals 2016, 9(3), 46; https://doi.org/10.3390/ph9030046
Received: 30 May 2016 / Revised: 19 June 2016 / Accepted: 25 July 2016 / Published: 28 July 2016
Activation of TRPV1, TRPA1 or TRPM8 channel expressed in the central terminal of dorsal root ganglion (DRG) neuron increases the spontaneous release of l-glutamate onto spinal dorsal horn lamina II (substantia gelatinosa; SG) neurons which play a pivotal role in regulating nociceptive transmission. The TRP channels are activated by various plant-derived chemicals. Although stereoisomers activate or modulate ion channels in a distinct manner, this phenomenon is not fully addressed for TRP channels. By applying the whole-cell patch-clamp technique to SG neurons of adult rat spinal cord slices, we found out that all of plant-derived chemicals, carvacrol, thymol, carvone and cineole, increase the frequency of spontaneous excitatory postsynaptic current, a measure of the spontaneous release of l-glutamate from nerve terminals, by activating TRP channels. The presynaptic activities were different between stereoisomers (carvacrol and thymol; (−)-carvone and (+)-carvone; 1,8-cineole and 1,4-cineole) in the extent or the types of TRP channels activated, indicating that TRP channels in the SG are activated by stereoisomers in a distinct manner. This result could serve to know the properties of the central terminal TRP channels that are targets of drugs for alleviating pain. View Full-Text
Keywords: TRPV1; TRPA1; TRPM8; plant-derived chemical; l-glutamate release; spinal dorsal horn; patch-clamp; rat TRPV1; TRPA1; TRPM8; plant-derived chemical; l-glutamate release; spinal dorsal horn; patch-clamp; rat
Show Figures

Figure 1

MDPI and ACS Style

Kumamoto, E.; Fujita, T. Differential Activation of TRP Channels in the Adult Rat Spinal Substantia Gelatinosa by Stereoisomers of Plant-Derived Chemicals. Pharmaceuticals 2016, 9, 46.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop